CA12 (NM_001218) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CA12 |
| Synonyms | CA-XII; CAXII; HsT18816; T18816 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001218 |
| ORF Size | 1062 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001218.5 |
| RefSeq Size | 4209 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 771 |
| UniProt ID | O43570 |
| Cytogenetics | 15q22.2 |
| Domains | carb_anhydrase |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Nitrogen metabolism |
| MW | 39.5 kDa |
| Summary | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. provided by RefSeq, Jun 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001218" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204810L1 | Lenti ORF clone of Human carbonic anhydrase XII (CA12), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC204810L2 | Lenti ORF clone of Human carbonic anhydrase XII (CA12), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC204810L3 | Lenti ORF clone of Human carbonic anhydrase XII (CA12), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC204810L4 | Lenti ORF clone of Human carbonic anhydrase XII (CA12), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RG204810 | CA12 (tGFP-tagged) - Human carbonic anhydrase XII (CA12), transcript variant 1 | 10 ug |
$489.00
$657.00
|
|
| SC119399 | CA12 (untagged)-Human carbonic anhydrase XII (CA12), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
Large pH oscillations promote host defense against human airways infection
Kim, Liao, Scales et al
J Exp Med (2021) 218 (4) DOI: 10.1084/jem.20201831
Kim, Liao, Scales et al
J Exp Med (2021) 218 (4) DOI: 10.1084/jem.20201831
Cited by 32
AI Summary: In this study, OriGene’s CA12 cDNA (RC208228) and CA12 shRNA (TF314894) products were used to manipulate CA12 expression in airway epithelial cells, enabling the authors to investigate the role of CA12 in airway surface pH oscillations and host defense against infection.
Governing effect of regulatory proteins for Cl(-)/HCO3(-) exchanger 2 activity
Jeong, Hong
Channels (Austin) (2015)
Jeong, Hong
Channels (Austin) (2015)
Cited by 12
AI Summary: OriGene products contributed to this study by providing the CA XII cDNA in a pCMV6-AC-Myc-DDK vector and the pCMV6-AC-mKate vector, which were used to express and analyze the role of carbonic anhydrase XII in regulating AE2 activity.
Snippet: CA XII cDNA in a pCMV6-AC-Myc-DDK vector was purchased from Origene Technologies (Rockville, MD). The cDNA encoding CA XII was excised from the original vectors and transferred to pCMV6-AC-mKate vectors (Origene Technologies). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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