CA12 (NM_001218) Human Untagged Clone

SKU SC119399

CA12 (untagged)-Human carbonic anhydrase XII (CA12), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CA12
• Synonyms: CA-XII; CAXII; HsT18816; T18816
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001218, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCCCCGGCGCAGCCTGCACGCGGCGGCCGTGCTCCTGCTGGTGATCTTAAAGGAACAGCCTTCCAGCC; CGGCCCCAGTGAACGGTTCCAAGTGGACTTATTTTGGTCCTGATGGGGAGAATAGCTGGTCCAAGAAGTA; CCCGTCGTGTGGGGGCCTGCTGCAGTCCCCCATAGACCTGCACAGTGACATCCTCCAGTATGACGCCAGC; CTCACGCCCCTCGAGTTCCAAGGCTACAATCTGTCTGCCAACAAGCAGTTTCTCCTGACCAACAATGGCC; ATTCAGTGAAGCTGAACCTGCCCTCGGACATGCACATCCAGGGCCTCCAGTCTCGCTACAGTGCCACGCA; GCTGCACCTGCACTGGGGGAACCCGAATGACCCGCACGGCTCTGAGCACACCGTCAGCGGACAGCACTTC; GCCGCCGAGCTGCACATTGTCCATTATAACTCAGACCTTTATCCTGACGCCAGCACTGCCAGCAACAAGT; CAGAAGGCCTCGCTGTCCTGGCTGTTCTCATTGAGATGGGCTCCTTCAATCCGTCCTATGACAAGATCTT; CAGTCACCTTCAACATGTAAAGTACAAAGGCCAGGAAGCATTCGTCCCGGGATTCAACATTGAAGAGCTG; CTTCCGGAGAGGACCGCTGAATATTACCGCTACCGGGGGTCCCTGACCACACCCCCTTGCAACCCCACTG; TGCTCTGGACAGTTTTCCGAAACCCCGTGCAAATTTCCCAGGAGCAGCTGCTGGCTTTGGAGACAGCCCT; GTACTGCACACACATGGACGACCCTTCCCCCAGAGAAATGATCAACAACTTCCGGCAGGTCCAGAAGTTC; GATGAGAGGCTGGTATACACCTCCTTCTCCCAAGTGCAAGTCTGTACTGCGGCAGGACTGAGTCTGGGCA; TCATCCTCTCACTGGCCCTGGCTGGCATTCTTGGCATCTGTATTGTGGTGGTGGTGTCCATTTGGCTTTT; CAGAAGGAAGAGTATCAAAAAAGGTGATAACAAGGGAGTCATTTACAAGCCAGCCACCAAGATGGAGACT; GAGGCCCACGCTTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001218 unedited; GGGTTTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTCGCCACGGCA; CCCAGGCTGCGCGCACGCGGTCCCGGTGTGCAGCTGGAGAGCGAGCGGCCACCGGGAGCC; CCCGGCACAGCCCGCGCCCGCCCCGCAGGAGCCCGCGAAGATGCCCCGGCGCAGCCTGCA; CGCGGCGGCCGTGCTCCTGCTGGTGATCTTAAAGGAACAGCCTTCCAGCCCGGCCCCAGT; GAACGGTTCCAAGTGGACTTATTTTGGTCCTGATGGGGAGAATAGCTGGTCCAAGAAGTA; CCCGTCGTGTGGGGGCCTGCTGCAGTCCCCCATAGACCTGCACAGTGACATCCTCCAGTA; TGACGCCAGCCTCACGCCCCTCGAGTTCCAAGGCTACAATCTGTCTGCCAACAAGCAGTT; TCTCCTGACCAACAATGGCCATTCAGTGAAGCTGAACCTGCCCTCGGACATGCACATCCA; GGGCCTCCAGTCTCGCTACAGTGCCACGCAGCTGCACCTGCACTGGGGGAACCCGAATGA; CCCGCACGGCTCTGAGCACACCGTCAGCGGACAGCACTTCGCCGCCGAGCTGCACATTGT; CCATTATAACTCAGACCTTTATCCTGACGCCAGCACTGCCAGCAACAAGTCAGAAGGCCT; CGCTGTCCTGGCTGTTCTCATTGAGATGGGCTCCTTCAATCCGTCCTATGACAAGATCTT; CAGTCACCTTCAACATGTAAAGTACAAAGGCCANGAAGCATTCGTCCCGGGATTCAACAT; TGAAGAGCTGCTTNCGGAGAGACCGCTNGATATTACCGCTACCGGNGGTCCCTGACACAC; CCCCTTTGCACCCCACTGTGCTCTGGACAGTTTTCCGAAACCCCCGTGCAATTTCCAGGA; GCAGCTGCTGGCTTTGGAGACGCCCTGTACTCACACCATGGACGACCCTCCCCAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001218 unedited; CGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAGATGGAGTCTCGCTCT; GTCACCCAGGCTAGAGTGCAGTGGCATGACCTTGGCTTACTGCAACCTCCGCCTCCTGGG; TTCAAGCGATTCCAGAAGAGAGAATTCTTAGAGTCAGAGGGAGGAGTAGAAGGAAAAAGA; TATTTAAAAAGCTATGCTTCAAGAGGACATTTCATGCTGTCAAAATGAGACTGTGAATCA; GAAAGTTCTCGGGGAACTGCAAGGTGCTCTCAACTAGGGGTCGGTTCCTTCTCAGTCATG; GCACTGACTCATCTCCACAGGGTTCTCACCTGCGGGAGGAAAATGGAGGAGTTGCGCCTG; TCAGAAACTGTCTGTGTGATTCGGGGAAGAATATGGAGTATCTTAGTAGCATTCCATTAT; TACTTGCCCCTAAATACATGATGCCAGCCCCCTGCACAGATAACCTCCTGCTTTTATAGC; TTGAAATATATTTGATCTAAACCACGATTTGACATCTTCAGAGAGAGAGAAGTAGATAAA; AGTCTCCATTCCAGGTTGGCAGTAGGGATCCTGCAGAAGTGGCTGAAATGAAATTTGGCC; TACAGAGAATAAGTTCTACAGTCATTTTGTTTCTGCCTATTTCCTCCAGATTCAAAGGCA; GATTGGGTCATCTCGGAACTCATGTCTCCTCCAGGACACAGCAACAACACATTTTCAAGG; AACAGCCTCATTATTTGTAAAATCTGTAGAGTATAAAACATAACTGCTCCACTGACAGGA; GGGNTGGATNTGGGACTNNATATGNATTATTCTGTCCTTACATTATGGCTGATAACAGAT; GTGATTCCATTCTGATCCAAGTGAGACCTNCCANACATCCTGTCTCTCATCTCTTGGCCT; CTAGCTTTCTCCTCTCTCCCACCAGAGTGGNACTGACTGCANCCCTAGCTGGCAGGCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001218
• Insert Size: 2790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001218.3, NP_001209.1
• RefSeq Size: 3992 bp
• RefSeq ORF: 1065 bp
• Locus ID: 771
• UniProt ID: O43570
• Cytogenetics: 15q22.2
• Domains: carb_anhydrase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Nitrogen metabolism
• Summary: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.