SRPX2 (NM_014467) Human Tagged ORF Clone

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SKU
RC204724
SRPX2 (Myc-DDK-tagged)-Human sushi-repeat containing protein, X-linked 2 (SRPX2)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SRPX2
Synonyms BPP; CBPS; PMGX; RESDX; SRPUL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204724 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGTCAGCTAACTCAAAGAGGAGCTCTCTTTCTGCTGTTCTTCCTAACTCCGGCAGTGACACCAA
CATGGTATGCAGGTTCTGGCTACTATCCGGATGAAAGCTACAATGAAGTATATGCAGAGGAGGTCCCACA
GGCTCCTGCCCTGGACTACCGAGTCCCCCGATGGTGTTATACATTAAATATCCAGGATGGAGAAGCCACA
TGCTACTCACCGAAGGGAGGAAATTATCACAGCAGCCTGGGCACGCGTTGTGAGCTCTCCTGTGACCGGG
GCTTTCGATTGATTGGAAGGAGGTCGGTGCAATGCCTGCCAAGCCGTCGTTGGTCTGGAACTGCCTACTG
CAGGCAGATGAGATGCCACGCACTACCATTCATCACTAGTGGCACTTACACCTGCACAAATGGAGTGCTT
CTTGACTCTCGCTGTGACTACAGCTGTTCCAGTGGCTACCACCTGGAAGGTGATCGCAGCCGAATCTGCA
TGGAAGATGGGAGATGGAGTGGAGGCGAGCCTGTATGTGTAGACATAGATCCCCCCAAGATCCGCTGTCC
CCACTCACGTGAGAAGATGGCAGAGCCAGAGAAATTGACTGCTCGAGTATACTGGGACCCACCGTTGGTG
AAAGATTCTGCTGATGGTACCATCACCAGGGTGACACTTCGGGGCCCTGAGCCTGGCTCTCACTTTCCCG
AAGGAGAGCATGTGATTCGTTACACTGCCTATGACCGAGCCTACAACCGGGCCAGCTGCAAGTTCATTGT
GAAAGTACAAGTGAGACGCTGCCCAACTCTGAAACCTCCGCAGCACGGCTACCTCACCTGCACCTCAGCG
GGGGACAACTATGGTGCCTCCTGTGAATACCACTGTGATGGCGGTTATGATCGCCAGGGGACACCCTCCC
GGGTCTGTCAGTCCAGCCGCCAGTGGTCAGGTTCACCACCAATCTGTGCTCCTATGAAGATTAACGTCAA
CGTCAACTCAGCTGCTGGTCTCTTGGATCAATTCTATGAGAAACAGCGACTCCTCATCATCTCAGCTCCT
GATCCTTCCAACCGATATTATAAAATGCAGATCTCTATGCTACAGCAATCCACCTGTGGACTGGATTTGC
GGCATGTGACCATCATTGAACTGGTGGGACAGCCACCTCAGGAGGTGGGGCGCATCCGGGAGCAACAGCT
GTCAGCCAACATCATCGAGGAGCTCAGGCAATTTCAGCGCCTCACTCGCTCCTACTTCAACATGGTGTTG
ATTGACAAGCAGGGTATTGACCGAGACCGCTACATGGAACCTGTCACCCCCGAGGAAATCTTCACATTCA
TTGATGACTACCTACTGAGCAATCAGGAGTTGACCCAGCGTCGGGAGCAAAGGGACATATGCGAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204724 protein sequence
Red=Cloning site Green=Tags(s)

MASQLTQRGALFLLFFLTPAVTPTWYAGSGYYPDESYNEVYAEEVPQAPALDYRVPRWCYTLNIQDGEAT
CYSPKGGNYHSSLGTRCELSCDRGFRLIGRRSVQCLPSRRWSGTAYCRQMRCHALPFITSGTYTCTNGVL
LDSRCDYSCSSGYHLEGDRSRICMEDGRWSGGEPVCVDIDPPKIRCPHSREKMAEPEKLTARVYWDPPLV
KDSADGTITRVTLRGPEPGSHFPEGEHVIRYTAYDRAYNRASCKFIVKVQVRRCPTLKPPQHGYLTCTSA
GDNYGASCEYHCDGGYDRQGTPSRVCQSSRQWSGSPPICAPMKINVNVNSAAGLLDQFYEKQRLLIISAP
DPSNRYYKMQISMLQQSTCGLDLRHVTIIELVGQPPQEVGRIREQQLSANIIEELRQFQRLTRSYFNMVL
IDKQGIDRDRYMEPVTPEEIFTFIDDYLLSNQELTQRREQRDICE

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_014467
ORF Size 1395 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014467.3
RefSeq Size 2206 bp
RefSeq ORF 1398 bp
Locus ID 27286
UniProt ID O60687
Cytogenetics Xq22.1
Domains CCP, HYR
Protein Families Druggable Genome, Secreted Protein
MW 53 kDa
Summary This gene encodes a secreted protein that contains three sushi repeat motifs. The encoded protein may play a role in the development of speech and language centers in the brain. This protein may also be involved in angiogenesis. Mutations in this gene are the cause of bilateral perisylvian polymicrogyria, rolandic epilepsy, speech dyspraxia and cognitive disability. provided by RefSeq, May 2010
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Documents
Resources
"NM_014467" in other vectors (5)
SKU Description Size Price
RC204724L3 Lenti ORF clone of Human sushi-repeat containing protein, X-linked 2 (SRPX2), Myc-DDK-tagged 10 ug
$757.00
RC204724L4 Lenti ORF clone of Human sushi-repeat containing protein, X-linked 2 (SRPX2), mGFP tagged 10 ug
$757.00
RG204724 SRPX2 (tGFP-tagged) - Human sushi-repeat containing protein, X-linked 2 (SRPX2) 10 ug
$489.00 $657.00
SC114976 SRPX2 (untagged)-Human sushi-repeat containing protein, X-linked 2 (SRPX2) 10 ug
$457.00
SC321108 SRPX2 (untagged)-Human sushi-repeat containing protein, X-linked 2 (SRPX2) 10 ug
$457.00

Plasmid Map for RC204724

Download sequence for

Cloning scheme for RC204724

Circular map for RC204724

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