RRAS2 (NM_012250) Human Tagged ORF Clone
SKU
RC204591
RRAS2 (Myc-DDK-tagged)-Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RRAS2 |
| Synonyms | NS12; TC21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012250 |
| ORF Size | 612 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012250.6 |
| RefSeq Size | 2360 bp |
| RefSeq ORF | 615 bp |
| Locus ID | 22800 |
| UniProt ID | P62070 |
| Cytogenetics | 11p15.2 |
| Domains | RAB, RAN, ras, RAS, RHO |
| Protein Families | Druggable Genome |
| Protein Pathways | MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction |
| MW | 23.4 kDa |
| Summary | This gene encodes a member of the R-Ras subfamily of Ras-like small GTPases. The encoded protein associates with the plasma membrane and may function as a signal transducer. This protein may play an important role in activating signal transduction pathways that control cell proliferation. Mutations in this gene are associated with the growth of certain tumors. Pseudogenes of this gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants. provided by RefSeq, Apr 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012250" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204591L3 | Lenti ORF clone of Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC204591L4 | Lenti ORF clone of Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG204591 | RRAS2 (tGFP-tagged) - Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC115460 | RRAS2 (untagged)-Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1 | 10 ug |
$300.00
|
|
| SC322652 | RRAS2 (untagged)-Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
Transfection of Sponge Cells and Intracellular Localization of Cancer-Related MYC, RRAS2, and DRG1 Proteins
Dominko, Talajić, Radić et al
Mar Drugs (2023) 21 (2) DOI: 10.3390/md21020119
Dominko, Talajić, Radić et al
Mar Drugs (2023) 21 (2) DOI: 10.3390/md21020119
Cited by 10
AI Summary: In this study, OriGene products—specifically the TrueORF cDNA clones for human MYC (RC200307), RRAS2 (RC201307), and DRG1 (RC204057)—were used to overexpress these cancer-related proteins in human and sponge cells to investigate their intracellular localization and functional conservation.
Snippet: luding growth, proliferation, and apoptosis [76,77]. The expression of c-myc is tightly regulated in normal conditions, while its dysregulation leads to enhanced levels of MYC, which contribute to tumorigenesis [78]. Members of the MYC family consist of six conserved boxes (MB0, MBI, MBII, MBIIIa, MBIIIb, and MBIV), named Myc homology boxes, the nuclear localization sequence (NLS), and the C-termi
Showing 1-2 of 2 papers.
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