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DRAM (DRAM1) (NM_018370) Human Tagged ORF Clone

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SKU
RC204574
DRAM1 (Myc-DDK-tagged)-Human DNA-damage regulated autophagy modulator 1 (DRAM1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DRAM
Synonyms DRAM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204574 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTGCTTCCTGAGGGGAATGGCTTTCGTCCCCTTCCTCTTGGTGACCTGGTCGTCAGCCGCCTTCA
TTATCTCCTACGTGGTCGCCGTGCTCTCCGGGCACGTCAACCCCTTCCTCCCGTATATCAGTGATACGGG
AACAACACCTCCAGAGAGTGGTATTTTTGGATTTATGATAAACTTCTCTGCATTTCTTGGTGCAGCCACG
ATGTATACAAGATACAAAATAGTACAGAAGCAAAATCAAACCTGCTATTTCAGCACTCCTGTTTTTAACT
TGGTGTCTTTAGTGCTTGGATTGGTGGGATGTTTCGGAATGGGCATTGTCGCCAATTTTCAGGAGTTAGC
TGTGCCAGTGGTTCATGACGGGGGCGCTCTTTTGGCCTTTGTCTGTGGTGTCGTGTACACGCTCCTACAG
TCCATCATCTCTTACAAATCATGTCCCCAGTGGAACAGTCTCTCGACATGCCACATACGGATGGTCATCT
CTGCCGTTTCTTGCGCAGCTGTCATCCCCATGATTGTCTGTGCTTCACTAATTTCCATAACCAAGCTGGA
GTGGAATCCAAGAGAAAAGGATTATGTATATCACGTAGTGAGTGCGATCTGTGAATGGACAGTGGCCTTT
GGTTTTATTTTCTACTTCCTAACTTTCATCCAAGATTTCCAGAGTGTCACCCTAAGGATATCCACAGAAA
TCAATGGTGATATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204574 protein sequence
Red=Cloning site Green=Tags(s)

MLCFLRGMAFVPFLLVTWSSAAFIISYVVAVLSGHVNPFLPYISDTGTTPPESGIFGFMINFSAFLGAAT
MYTRYKIVQKQNQTCYFSTPVFNLVSLVLGLVGCFGMGIVANFQELAVPVVHDGGALLAFVCGVVYTLLQ
SIISYKSCPQWNSLSTCHIRMVISAVSCAAVIPMIVCASLISITKLEWNPREKDYVYHVVSAICEWTVAF
GFIFYFLTFIQDFQSVTLRISTEINGDI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018370
ORF Size 714 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018370.3
RefSeq Size 3553 bp
RefSeq ORF 717 bp
Locus ID 55332
UniProt ID Q8N682, A0A024RBF9
Cytogenetics 12q23.2
Protein Families Transmembrane
MW 26.3 kDa
Summary This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. provided by RefSeq, Jul 2008
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"NM_018370" in other vectors (5)
SKU Description Size Price
RC204574L3 Lenti ORF clone of Human DNA-damage regulated autophagy modulator 1 (DRAM1), Myc-DDK-tagged 10 ug
$600.00
RC204574L4 Lenti ORF clone of Human DNA-damage regulated autophagy modulator 1 (DRAM1), mGFP tagged 10 ug
$600.00
RG204574 DRAM1 (tGFP-tagged) - Human DNA-damage regulated autophagy modulator 1 (DRAM1) 10 ug
$489.00 $500.00
SC113578 DRAM1 (untagged)-Human DNA-damage regulated autophagy modulator 1 (DRAM1) 10 ug
$150.00
SC321673 DRAM1 (untagged)-Human DNA-damage regulated autophagy modulator 1 (DRAM1) 10 ug
$300.00

Plasmid Map for RC204574

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204574
Circular map for RC204574

file-alt Citations

DNA Damage-Regulated Autophagy Modulator1 (DRAM1)-induced lipophagy facilitates Toxoplasma gondii nutrient acquisition and infection
Hou, Huang, Su et al
Microbiological Research (2025)
Cited by 1
AI Summary: OriGene contributed to the study by providing the human DRAM1 expression plasmid (pCMV-hDram1-Flag, RC204574) and mouse DRAM1 expression plasmid (pCMV-mDram1-Flag, MR220640), which were used to investigate the role of DRAM1 in Toxoplasma gondii infection.
Snippet: The human DRAM1 expression plasmid pCMV-hDram1-Flag (RC204574) and mouse DRAM1 expression plasmid pCMV-mDram1-Flag (MR220640) was purchased from ORIGENE. The siRNA sequences used were as follows: human Dram1 UGAUAAACUUCUCUGCAUUUCTT (1),
Showing 1-1 of 1 papers.
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