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FBXO30 (NM_032145) Human Tagged ORF Clone

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SKU
RC204568
FBXO30 (Myc-DDK-tagged)-Human F-box protein 30 (FBXO30)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $682.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXO30
Synonyms Fbx30
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204568 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGAGCTGCAGCATTCCCATTGTGTGAATTGTGTCAGTAGACGGTGCATGACCAGGCCAGAGC
CAGGGATTTCCTGTGATTTGATTGGTTGTCCATTGGTTTGTGGTGCAGTTTTCCATTCTTGTAAAGCTGA
TGAGCATCGACTTTTATGTCCATTTGAACGAGTGCCTTGCTTAAATAGTGACTTTGGATGTCCATTTACC
ATGGCCCGAAATAAAGTTGCTGAACATCTAGAAATGTGTCCTGCAAGTGTGGTGTGCTGTACTATGGAAT
GGAATCGATGGCCAGTTAGTTATGCAGACCGGAAATCATATGAAAATCTAAGCAGAGATGTCGATGAAGT
GGCACAATTGGATATGGCCTTGGCTCTTCAAGACCAAAGGATGCTCTTAGAATCCCTCAAAGTAGCCACC
ATGATGTCAAAAGCAACTGATAAAGTATCCAAACCTAGAGAACAAATCTCAGTTAAATCAAGTGTCCCAG
AAATACCACATGCTAATGGTTTAGTGTCTGTTGATGAAGAATCTTATGGTGCACTTTATCAAGCTACTGT
AGAAACAACCAGAAGTTTGGCTGCTGCTTTGGATATCCTGAATACTGCTACAAGAGACATTGGCATGTTA
AATACAAGTGTCCCAAATGACATGGATGAACAGCAAAATGCGAGAGAAAGCTTAGAGGATCAAAACTTGA
AAGACCAAGATCATCTTTATGAGGAGGAAATAGGAGCAGTAGGTGGAATTGACTACAATGACACAAATCA
GAATGCCCAGTCTGAACAAAATGGTTCAAGTGATTTATTATGTGACTTGAATACAAGTTCTTATGACACT
TCTGCTCTTTGTAATGGCTTTCCTTTGGAAAATATATGTACCCAGGTCATAGACCAGAATCAGAATTTAC
ATGGTGATTCAAAACAAAGTAACTTAACAAATGGAGACTGTGTGGCATCATCAGATGGCACTTCAAAACC
TTCCAGCTCACTTGCGGTGGCAGCACAACTTAGGGAAATAATACCATCCAGTGCTTTGCCTAATGGCACA
GTTCAGCATATCCTCATGCCAGATGATGAAGGTGAAGGTGAATTGTGTTGGAAAAAAGTAGACTTAGGGG
ACGTGAAGAATGTGGATGTCTTATCTTTCAGTCATGCTCCTTCATTCAATTTTCTTTCTAATTCATGTTG
GTCTAAACCAAAGGAAGATAAAGCAGTAGATACATCAGATTTGGAAGTTGCAGAAGATCCTATGGGCCTC
CAAGGAATAGATCTGATCACAGCAGCATTGCTTTTTTGTCTAGGAGATTCTCCAGGAGGGAGGGGTATAT
CTGATAGCCGCATGGCTGATATTTATCACATTGACGTTGGGACTCAGACTTTTTCACTTCCATCTGCAAT
ATTAGCTACAAGTACAATGGTTGGGGAGATAGCTTCAGCTTCAGCTTGTGATCATGCCAATCCACAGCTT
TCAAATCCAAGTCCGTTTCAGACACTTGGGCTGGATTTAGTATTGGAATGTGTCGCTAGGTACCAACCCA
AGCAGCGTTCAATGTTTACCTTTGTGTGTGGACAGTTATTTAGAAGGAAAGAATTTTCTTCCCACTTTAA
GAATGTGCATGGTGACATTCATGCTGGACTCAATGGCTGGATGGAACAGAGGTGCCCTTTAGCTTACTAT
GGTTGTACCTATTCTCAGCGTAGATTTTGTCCATCAATACAAGGAGCAAAGATTATACATGACCGCCATT
TGAGGTCATTTGGAGTTCAGCCATGTGTATCTACAGTATTAGTGGAGCCTGCTAGAAACTGTGTGTTGGG
ATTACATAATGACCATCTAAGTAGTCTTCCTTTTGAGGTCCTGCAGCATATTGCAGGCTTTCTCGATGGC
TTCAGCTTATGTCAGCTCTCATGTGTATCCAAGTTAATGAGGGATGTGTGTGGCAGCCTGCTTCAGTCTC
GTGGCATGGTCATACTGCAGTGGGGGAAAAGGAAGTATCCAGAAGGAAATTCATCATGGCAGATAAAAGA
AAAGGTATGGCGATTTAGTACTGCATTTTGTTCTGTTAATGAATGGAAATTTGCTGACATCCTAAGCATG
GCAGACCACTTGAAGAAATGCAGTTACAATGTTGTCGAGAAACGGGAGGAAGCAATCCCTTTGCCATGTA
TGTGTGTGACACGAGAACTCACTAAAGAAGGACGTTCACTACGCTCAGTTTTAAAACCTGTACTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204568 protein sequence
Red=Cloning site Green=Tags(s)

MEEELQHSHCVNCVSRRCMTRPEPGISCDLIGCPLVCGAVFHSCKADEHRLLCPFERVPCLNSDFGCPFT
MARNKVAEHLEMCPASVVCCTMEWNRWPVSYADRKSYENLSRDVDEVAQLDMALALQDQRMLLESLKVAT
MMSKATDKVSKPREQISVKSSVPEIPHANGLVSVDEESYGALYQATVETTRSLAAALDILNTATRDIGML
NTSVPNDMDEQQNARESLEDQNLKDQDHLYEEEIGAVGGIDYNDTNQNAQSEQNGSSDLLCDLNTSSYDT
SALCNGFPLENICTQVIDQNQNLHGDSKQSNLTNGDCVASSDGTSKPSSSLAVAAQLREIIPSSALPNGT
VQHILMPDDEGEGELCWKKVDLGDVKNVDVLSFSHAPSFNFLSNSCWSKPKEDKAVDTSDLEVAEDPMGL
QGIDLITAALLFCLGDSPGGRGISDSRMADIYHIDVGTQTFSLPSAILATSTMVGEIASASACDHANPQL
SNPSPFQTLGLDLVLECVARYQPKQRSMFTFVCGQLFRRKEFSSHFKNVHGDIHAGLNGWMEQRCPLAYY
GCTYSQRRFCPSIQGAKIIHDRHLRSFGVQPCVSTVLVEPARNCVLGLHNDHLSSLPFEVLQHIAGFLDG
FSLCQLSCVSKLMRDVCGSLLQSRGMVILQWGKRKYPEGNSSWQIKEKVWRFSTAFCSVNEWKFADILSM
ADHLKKCSYNVVEKREEAIPLPCMCVTRELTKEGRSLRSVLKPVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032145
ORF Size 2235 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032145.5
RefSeq Size 4420 bp
RefSeq ORF 2238 bp
Locus ID 84085
UniProt ID Q8TB52
Cytogenetics 6q24.3
Domains F-box
Protein Families Druggable Genome
MW 82.3 kDa
Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it is upregulated in nasopharyngeal carcinoma. provided by RefSeq, Jul 2008
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"NM_032145" in other vectors (7)
SKU Description Size Price
RC204568L1 Lenti ORF clone of Human F-box protein 30 (FBXO30), Myc-DDK-tagged 10 ug
$982.00
RC204568L2 Lenti ORF clone of Human F-box protein 30 (FBXO30), mGFP tagged 10 ug
$982.00
RC204568L3 Lenti ORF clone of Human F-box protein 30 (FBXO30), Myc-DDK-tagged 10 ug
$982.00
RC204568L4 Lenti ORF clone of Human F-box protein 30 (FBXO30), mGFP tagged 10 ug
$982.00
RG204568 FBXO30 (tGFP-tagged) - Human F-box protein 30 (FBXO30) 10 ug
$789.00 $882.00
SC106014 FBXO30 (untagged)-Human F-box protein 30 (FBXO30) 10 ug
$683.00
SC322525 FBXO30 (untagged)-Human F-box protein 30 (FBXO30) 10 ug
$683.00

Plasmid Map for RC204568

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204568
Circular map for RC204568

file-alt Citations

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