MITD1 (NM_138798) Human Tagged ORF Clone

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SKU
RC204503
MITD1 (Myc-DDK-tagged)-Human MIT, microtubule interacting and transport, domain containing 1 (MITD1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MITD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204503 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAGTCCGGGCTGAGGCAGGACCCGCAGAGCACAGCTGCAGCCACTGTGCTAAAGCGGGCAGTAG
AACTAGATTCGGAGTCGCGGTATCCGCAGGCTCTGGTGTGTTACCAAGAGGGGATTGATCTGCTCCTGCA
GGTTCTGAAAGGTACCAAAGATAATACTAAGAGATGTAATCTCAGAGAAAAAATTTCCAAATACATGGAC
AGAGCGGAAAACATAAAGAAGTACTTGGACCAAGAAAAAGAAGATGGAAAATATCACAAGCAAATTAAAA
TAGAAGAGAATGCAACAGGTTTCAGTTATGAGTCACTTTTTCGCGAATACCTTAATGAGACAGTTACAGA
AGTTTGGATAGAAGATCCTTATATTAGACATACTCATCAGCTGTATAACTTTCTTCGATTTTGTGAGATG
CTTATTAAGAGACCATGTAAAGTAAAAACTATTCACCTTCTCACCTCTCTGGATGAAGGCATTGAGCAAG
TGCAGCAAAGTAGAGGCCTGCAAGAAATAGAAGAGTCACTCAGGAGTCACGGAGTGCTGTTGGAAGTTCA
ATACTCTTCTTCAATACATGACCGAGAAATTAGGTTCAACAATGGATGGATGATTAAGATTGGAAGGGGA
CTTGATTATTTTAAGAAACCACAGAGTCGTTTTTCCCTTGGATATTGTGATTTTGATTTAAGACCATGTC
ATGAAACAACAGTAGACATTTTTCATAAGAAGCATACAAAAAATATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204503 protein sequence
Red=Cloning site Green=Tags(s)

MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMD
RAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWIEDPYIRHTHQLYNFLRFCEM
LIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSHGVLLEVQYSSSIHDREIRFNNGWMIKIGRG
LDYFKKPQSRFSLGYCDFDLRPCHETTVDIFHKKHTKNI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138798
ORF Size 747 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138798.3
RefSeq Size 963 bp
RefSeq ORF 750 bp
Locus ID 129531
UniProt ID Q8WV92
Cytogenetics 2q11.2
MW 29.3 kDa
Summary Abscission, the separation of daughter cells at the end of cytokinesis, is effected by endosomal sorting complexes required for transport III (ESCRT-III). The protein encoded by this gene functions as a homodimer, with the N-termini binding to a subset of ESCRT-III subunits and the C-termini binding to membranes. The encoded protein regulates ESCRT-III activity and is required for proper cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Feb 2016
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Documents
Resources
"NM_138798" in other vectors (4)
SKU Description Size Price
RC204503L3 Lenti ORF clone of Human MIT, microtubule interacting and transport, domain containing 1 (MITD1), Myc-DDK-tagged 10 ug
$600.00
RC204503L4 Lenti ORF clone of Human MIT, microtubule interacting and transport, domain containing 1 (MITD1), mGFP tagged 10 ug
$600.00
RG204503 MITD1 (tGFP-tagged) - Human MIT, microtubule interacting and transport, domain containing 1 (MITD1) 10 ug
$489.00 $500.00
SC123153 MITD1 (untagged)-Human MIT, microtubule interacting and transport, domain containing 1 (MITD1) 10 ug
$300.00

Plasmid Map for RC204503

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Cloning scheme for RC204503

Circular map for RC204503

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