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PP2A-beta (PPP2CB) (NM_001009552) Human Tagged ORF Clone

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SKU
RC204491
PPP2CB (Myc-DDK-tagged)-Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "PP2A-beta or NM_001009552"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PP2A-beta
Synonyms PP2Abeta; PP2CB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204491 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGACAAGGCGTTCACCAAGGAGCTGGACCAGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGA
ACGAGAACCAAGTGCGGACGCTGTGCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGA
GGTTCGTTGCCCTGTTACTGTCTGTGGAGATGTGCATGGTCAATTTCATGATCTTATGGAACTCTTTAGA
ATTGGTGGAAAATCACCGGATACAAACTACTTATTCATGGGTGACTATGTAGACAGAGGATATTATTCAG
TGGAGACTGTGACTCTTCTTGTAGCATTAAAGGTGCGTTATCCAGAACGCATTACAATATTGAGAGGAAA
TCACGAAAGCCGACAAATTACCCAAGTATATGGCTTTTATGATGAATGTCTGCGAAAGTATGGGAATGCC
AACGTTTGGAAATATTTTACAGATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGATGGACAGATAT
TCTGCCTCCATGGTGGCCTCTCTCCATCCATAGACACACTGGATCATATAAGAGCCCTGGATCGTTTACA
GGAAGTTCCACATGAGGGCCCAATGTGTGATCTGTTATGGTCAGATCCAGATGATCGTGGTGGATGGGGT
ATTTCACCACGTGGTGCTGGCTACACATTTGGACAAGACATTTCTGAAACCTTTAACCATGCCAATGGTC
TCACACTGGTTTCTCGTGCCCACCAGCTTGTAATGGAGGGATACAATTGGTGTCATGATCGGAATGTGGT
TACCATTTTCAGTGCACCCAATTACTGTTATCGTTGTGGGAACCAGGCTGCTATCATGGAATTAGATGAC
ACTTTAAAATATTCCTTCCTTCAATTTGACCCAGCGCCTCGTCGTGGTGAGCCTCATGTTACACGGCGCA
CCCCAGACTACTTCCTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204491 protein sequence
Red=Cloning site Green=Tags(s)

MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFR
IGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNA
NVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWG
ISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDD
TLKYSFLQFDPAPRRGEPHVTRRTPDYFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001009552
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001009552.2
RefSeq Size 1987 bp
RefSeq ORF 930 bp
Locus ID 5516
UniProt ID P62714, A0A140VJS0
Cytogenetics 8p12
Protein Families Druggable Genome, Phosphatase
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
MW 35.6 kDa
Summary This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. provided by RefSeq, Mar 2010
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"NM_001009552" in other vectors (6)
SKU Description Size Price
RC204491L1 Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), Myc-DDK-tagged 10 ug
$600.00
RC204491L2 Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), mGFP tagged 10 ug
$600.00
RC204491L3 Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), Myc-DDK-tagged 10 ug
$600.00
RC204491L4 Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), mGFP tagged 10 ug
$600.00
RG204491 PPP2CB (tGFP-tagged) - Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB) 10 ug
$489.00 $500.00
SC127985 PPP2CB (untagged)-Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB) 10 ug
$300.00

Plasmid Map for RC204491

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204491
Circular map for RC204491

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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