SLC2A6 (NM_017585) Human Tagged ORF Clone

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SKU
RC204391
SLC2A6 (Myc-DDK-tagged)-Human solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript variant 1
  $472.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC2A6
Synonyms GLUT6; GLUT9; HSA011372
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204391 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGAGCCGCTGCTGGGAGCCGAGGGCCCGGACTACGACACCTTCCCCGAGAAGCCGCCCCCGTCGC
CAGGGGACAGGGCGCGGGTCGGGACCCTGCAGAACAAAAGGGTGTTCCTGGCCACCTTCGCCGCAGTGCT
CGGCAATTTCAGCTTTGGGTATGCCCTGGTCTACACATCCCCTGTCATCCCAGCCCTGGAGCGCTCCTTG
GATCCTGACCTGCATCTGACCAAATCCCAGGCATCCTGGTTTGGGTCCGTGTTCACCCTGGGAGCAGCGG
CCGGAGGCCTGAGTGCCATGATCCTCAACGACCTCCTGGGCCGGAAGCTGAGCATCATGTTCTCAGCTGT
GCCGTCGGCGGCCGGCTATGCGCTCATGGCGGGTGCGCACGGCCTCTGGATGCTGCTGCTCGGAAGGACG
CTGACGGGCTTCGCCGGGGGGCTCACAGCTGCCTGCATCCCGGTGTACGTGTCTGAGATTGCTCCCCCAG
GCGTTCGTGGGGCTCTGGGGGCCACACCCCAGCTCATGGCAGTGTTCGGATCCCTGTCCCTCTACGCCCT
TGGCCTCCTGCTGCCGTGGCGCTGGCTGGCTGTGGCCGGGGAGGCGCCTGTGCTCATCATGATCCTGCTG
CTCAGCTTCATGCCCAACTCGCCGCGCTTCCTGCTCTCTCGGGGCAGGGACGAAGAGGCCCTGCGGGCGC
TGGCCTGGCTGCGTGGGACGGACGTCGATGTCCACTGGGAGTTCGAGCAGATCCAGGACAACGTCCGGAG
ACAGAGCAGCCGAGTATCGTGGGCTGAGGCACGGGCCCCCCACGTGTGCCGGCCCATCACCGTGGCCTTG
CTGATGCGCCTCCTGCAGCAGCTGACGGGCATCACGCCCATCCTGGTCTACCTGCAGTCCATCTTCGACA
GCACCGCTGTCCTGCTGCCCCCCAAGGACGACGCAGCCATCGTTGGGGCCGTGCGGCTCCTGTCCGTGCT
GATCGCCGCCCTCACCATGGACCTCGCAGGCCGCAAGGTGCTGCTCTTCGTCTCAGCGGCCATCATGTTT
GCTGCCAACCTGACTCTGGGGCTGTACATCCACTTTGGCCCCAGGCCTCTGAGCCCCAACAGCACTGCGG
GCCTGGAAAGCGAGTCCTGGGGGGACTTGGCGCAGCCCCTGGCAGCACCCGCTGGCTACCTCACCCTGGT
GCCCCTGCTGGCCACCATGCTCTTCATCATGGGCTACGCCGTGGGCTGGGGTCCCATCACCTGGCTGCTC
ATGTCTGAGGTCCTGCCCCTGCGTGCCCGTGGCGTGGCCTCAGGGCTCTGCGTGCTGGCCAGCTGGCTCA
CCGCCTTCGTCCTCACCAAGTCCTTCCTGCCAGTGGTGAGCACCTTCGGCCTCCAGGTGCCTTTCTTCTT
CTTCGCGGCCATCTGCTTGGTGAGCCTGGTGTTCACAGGCTGCTGTGTGCCCGAGACCAAGGGACGGTCC
CTGGAGCAGATCGAGTCCTTCTTCCGCACGGGGAGAAGGTCCTTCTTGCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204391 protein sequence
Red=Cloning site Green=Tags(s)

MQEPLLGAEGPDYDTFPEKPPPSPGDRARVGTLQNKRVFLATFAAVLGNFSFGYALVYTSPVIPALERSL
DPDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAAGYALMAGAHGLWMLLLGRT
LTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALGLLLPWRWLAVAGEAPVLIMILL
LSFMPNSPRFLLSRGRDEEALRALAWLRGTDVDVHWEFEQIQDNVRRQSSRVSWAEARAPHVCRPITVAL
LMRLLQQLTGITPILVYLQSIFDSTAVLLPPKDDAAIVGAVRLLSVLIAALTMDLAGRKVLLFVSAAIMF
AANLTLGLYIHFGPRPLSPNSTAGLESESWGDLAQPLAAPAGYLTLVPLLATMLFIMGYAVGWGPITWLL
MSEVLPLRARGVASGLCVLASWLTAFVLTKSFLPVVSTFGLQVPFFFFAAICLVSLVFTGCCVPETKGRS
LEQIESFFRTGRRSFLR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017585
ORF Size 1521 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017585.4
RefSeq Size 2563 bp
RefSeq ORF 1524 bp
Locus ID 11182
UniProt ID Q9UGQ3
Cytogenetics 9q34.2
Protein Families Transmembrane
MW 54.5 kDa
Summary Hexose transport into mammalian cells is catalyzed by a family of membrane proteins, including SLC2A6, that contain 12 transmembrane domains and a number of critical conserved residues.supplied by OMIM, Jul 2002
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Documents
Resources
"NM_017585" in other vectors (6)
SKU Description Size Price
RC204391L1 Lenti ORF clone of Human solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript variant 1, Myc-DDK-tagged 10 ug
$772.00
RC204391L2 Lenti ORF clone of Human solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript variant 1, mGFP tagged 10 ug
$772.00
RC204391L3 Lenti ORF clone of Human solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript variant 1, Myc-DDK-tagged 10 ug
$772.00
RC204391L4 Lenti ORF clone of Human solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript variant 1, mGFP tagged 10 ug
$772.00
RG204391 SLC2A6 (tGFP-tagged) - Human solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript variant 1 10 ug
$489.00 $672.00
SC122863 SLC2A6 (untagged)-Human solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript variant 1 10 ug
$473.00

Plasmid Map for RC204391

Download sequence for

Cloning scheme for RC204391

Circular map for RC204391

file-alt Citations

Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1
Kavanagh Williamson, Coombes, Juszczak et al
Viruses (2018) 10 (3) DOI: 10.3390/v10030114
AI Summary: OriGene plasmids RC204391 and RC227402 were used to express GLUT6 in 293T cells, enabling the authors to confirm the specificity of GLUT6 antibody detection in their study of glucose transporter expression in HIV-1 infected CD4+ T cells.
Snippet: 845), Tubulin (Abcam Ab7291) and HSP90 (Santa Cruz H-114 and 4F10). The specificities of GLUT antibodies were confirmed by the following observations: the unique detection of GLUT6 expressed from the OriGene plasmids RC204391 and RC227402 with the B-3 antibody after transfection of 293T cells, and the unique detection of GLUT3 in 293T cells [43] with the G-5 and Ab191071 antibodies [44]. The speci
FigureFigureFigure
Showing 1-1 of 1 papers.
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