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RPUSD4 (NM_032795) Human Tagged ORF Clone

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SKU
RC204352
RPUSD4 (Myc-DDK-tagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPUSD4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204352 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCCCAGGTGGAGCGCGTCGGGCCCCTGGATCCGGGGAAACGGCCAAGGTTGCGGGAGTCTCT
TCACTCTCGTCTCAAAGCCATTTTGTGCCGCTGCCGCTGCCTCTACGGCCATAAATGCCCAGAGATTAGC
GGAGAAGCTCCGAGCCCAGAAACGGGAACAAGACACAAAGAAGGAGCCGGTGTCCACAAACGCTGTTCAG
CGGAGAGTGCAAGAAATAGTGCGGTTCACACGGCAGCTGCAGCGAGTCCACCCCAACGTGCTTGCTAAGG
CACTGACCCGAGGAATTCTCCACCAGGACAAGAACCTTGTGGTCATCAATAAGCCCTACGGTCTCCCTGT
GCATGGTGGCCCTGGGGTCCAGCTCTGCATCACTGATGTACTACCTATCCTGGCAAAGATGCTTCATGGC
CACAAGGCAGAGCCCTTGCATCTGTGCCACCGGCTGGACAAGGAAACCACAGGTGTAATGGTGTTGGCTT
GGGACAAGGACATGGCACATCAAGTCCAAGAGTTGTTTAGAACCCGTCAGGTGGTGAAGAAGTACTGGGC
CATCACTGTGCATGTCCCCATGCCCTCAGCAGGAGTCGTGGACATCCCCATTGTGGAGAAGGAGGCGCAA
GGCCAGCAGCAACACCACAAGATGACATTGTCCCCGAGCTACCGCATGGACGATGGGAAAATGGTGAAAG
TGCGGCGCAGCCGGAATGCGCAAGTTGCTGTAACTCAGTACCAGGTGCTCAGCAGCACTCTCTCCTCCGC
CCTCGTGGAGCTCCAGCCCATCACTGGAATAAAACATCAGCTTCGAGTTCACTTGTCTTTTGGATTGGAT
TGTCCAATCCTTGGTGATCACAAGTACTCAGACTGGAATAGGTTGGCCCCCCAGAAGCTGTCTGTGGGCA
CCCTGAAGAAGCTGGGGCTAGAACAGTCGAAGGCCCGCTACATCCCCCTTCACCTGCACGCCCGGCAGCT
GATCCTGCCTGCCCTGGGGTCCGGGAAGGAGGAACTCAACTTGGTCTGCAAACTTCCTCGCTTCTTTGTG
CATTCCCTGCACCGCCTGCGTTTAGAGATGCCAAATGAGGATCAAAATGAGAACAATGAAGCCAAGTGTC
TGGGAGCACAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204352 protein sequence
Red=Cloning site Green=Tags(s)

MAAPRWSASGPWIRGNGQGCGSLFTLVSKPFCAAAAASTAINAQRLAEKLRAQKREQDTKKEPVSTNAVQ
RRVQEIVRFTRQLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGPGVQLCITDVLPILAKMLHG
HKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQ
GQQQHHKMTLSPSYRMDDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLD
CPILGDHKYSDWNRLAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLILPALGSGKEELNLVCKLPRFFV
HSLHRLRLEMPNEDQNENNEAKCLGAQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032795
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032795.3
RefSeq Size 2527 bp
RefSeq ORF 1134 bp
Locus ID 84881
UniProt ID Q96CM3
Cytogenetics 11q24.2
Domains PseudoU_synth_2
MW 42.2 kDa
Summary Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome (PubMed:27974379). Acts on position 39 in mitochondrial tRNA(Phe) (PubMed:28082677). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed:27667664).UniProtKB/Swiss-Prot Function
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"NM_032795" in other vectors (4)
SKU Description Size Price
RC204352L3 Lenti ORF clone of Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC204352L4 Lenti ORF clone of Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, mGFP tagged 10 ug
$757.00
RG204352 RPUSD4 (tGFP-tagged) - Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 10 ug
$489.00 $657.00
SC103625 RPUSD4 (untagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 10 ug
$457.00

Plasmid Map for RC204352

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204352
Circular map for RC204352

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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