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RPUSD4 (NM_032795) Human Untagged Clone

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SKU
SC103625
RPUSD4 (untagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RPUSD4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_032795, the custom clone sequence may differ by one or more nucleotides


ATGGCGGCGCCCAGGTGGAGCGCGTCGGGCCCCTGGATCCGGGGAAACGGCCAAGGTTGCGGGAGTCTCT
TCACTCTCGTCTCAAAGCCATTTTGTGCCGCTGCCGCTGCCTCTACGGCCATAAATGCCCAGAGATTAGC
GGAGAAGCTCCGAGCCCAGAAACGGGAACAAGACACAAAGAAGGAGCCGGTGTCCACAAACGCTGTTCAG
CGGAGAGTGCAAGAAATAGTGCGGTTCACACGGCAGCTGCAGCGAGTCCACCCCAACGTGCTTGCTAAGG
CACTGACCCGAGGAATTCTCCACCAGGACAAGAACCTTGTGGTCATCAATAAGCCCTACGGTCTCCCTGT
GCATGGTGGCCCTGGGGTCCAGCTCTGCATCACTGATGTACTACCTATCCTGGCAAAGATGCTTCATGGC
CACAAGGCAGAGCCCTTGCATCTGTGCCACCGGCTGGACAAGGAAACCACAGGTGTAATGGTGTTGGCTT
GGGACAAGGACATGGCACATCAAGTCCAAGAGTTGTTTAGAACCCGTCAGGTGGTGAAGAAGTACTGGGC
CATCACTGTGCATGTCCCCATGCCCTCAGCAGGAGTCGTGGACATCCCCATTGTGGAGAAGGAGGCGCAA
GGCCAGCAGCAACACCACAAGATGACATTGTCCCCGAGCTACCGCATGGACGATGGGAAAATGGTGAAAG
TGCGGCGCAGCCGGAATGCGCAAGTTGCTGTAACTCAGTACCAGGTGCTCAGCAGCACTCTCTCCTCCGC
CCTCGTGGAGCTCCAGCCCATCACTGGAATAAAACATCAGCTTCGAGTTCACTTGTCTTTTGGATTGGAT
TGTCCAATCCTTGGTGATCACAAGTACTCAGACTGGAATAGGTTGGCCCCCCAGAAGCTGTCTGTGGGCA
CCCTGAAGAAGCTGGGGCTAGAACAGTCGAAGGCCCGCTACATCCCCCTTCACCTGCACGCCCGGCAGCT
GATCCTGCCTGCCCTGGGGTCCGGGAAGGAGGAACTCAACTTGGTCTGCAAACTTCCTCGCTTCTTTGTG
CATTCCCTGCACCGCCTGCGTTTAGAGATGCCAAATGAGGATCAAAATGAGAACAATGAAGCCAAGTGTC
TGGGAGCACAGTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_032795 unedited
AGTATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCCCGCCCTT
CCTTGTAAGATGGCGGCGCCCAGGTGGAGCGCGTCGGGCCCCTGGATCCGGGGAAACGGC
CAAGGTTGCGGGAGTCTCTTCACTCTCGTCTCAAAGCCATTTTGTGCCGCTGCCGCTGCC
TCTACGGCCATAAATGCCCGGAGATTAGCGGAGAAGCTCCGAGCCCAGAAACGGGAACAA
GACACAAAGAAGGAGCCGGTGTCCACAAACGCTGTTCAGCGGAGAGTGCAAGAAATAGTG
CGGTTCACACGGCAGCTGCAGCGAGTCCACCCCAACGTGCTTGCTAAGGCACTGACCCGA
GGAATTCTCCACCAGGACAAGAACCTTGTGGTCATCAATAAGCCCTACGGTCTCCCTGTG
CATGGTGGCCCTGGGGTCCAGCTCTGCATCACTGATGTACTACCTATCCTGGCAAAGATG
CTTCATGGCCACAAGGCAGAGCCCTTGCATCTGTGCCACCGGCTGGACAAGGAAACCACA
GGTGTAATGGTGTTGGCTTGGGACAAGGACATGGCACATCAAGTCCAAGAGTTGTTTAGA
ACCCGTCAGGTGGTGAAGAAGTACTGGGCCATCACTGTGCATGTCCCCATGCCCTCAGCA
GGAGTCGTGGACATCCCCATTGTGGAGAAGGAGGCGCAAGGCCAGCAGCAACACCACAAG
ATGACATTGTCCCCGAGCTACCGCATGGACGATGGGAAAATGGTGAAAGTGCGGCGCAGC
CCGAATGCGCAAGNTGCTGTAACTCAGTACCAGGTGCTCAGCAGCACTCTCTCCTNCGNC
CTCGTGGAGCTCCAGCCCATCACTGGNNATANACATCAGCTTCGAGTCACTTGTCCTTTG
NATGGNATNGNNCATNNCTNGTGATCACAGTACTCAGACTGGAATAGGTGGNCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032795 unedited
TTTGGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGCTTTCAAGTT
AAATTTATTATGCACAGTAATGGGTACCATGGAGACAGGGAAAGGGCTAGAAAGTGGCAT
CATCTATACAGAGGGAACATATAAAGACTGTCCCGGGGTGTACCCCCCTAACTGTGCTGT
CACTCCAACTCTGTCAGAAAGAAAAAGTAGTGTGAGGTGTATTTGTTTGCAAGAAACAAG
GTGGAAACTAGATGGACCAGCTGAGCAGGGAGGCCAAGGTCATTTCCATCTATCCCTAAT
GGTGTTCAGTATAGCCCTCTCCTTCCTGAGACCTAAGAACAGAAGATACGCTTCCACAAC
AGAGATTTTTGATTTTTTAATGGCAGCACTCTCATAACTACATTCACGGACCAACCAAAG
CCATCTCCAAGCCTAGAAAGGATACATACTACACGCAGCGAAAATGCCAACCACTGATCC
AGAATTTTCAAGATGGCACCTTTCCCCAAACTTCTATACCATTCATTAAACATAGACTGC
AAACTGCTAGCCTGTAGACCAAATTCAACCTCTAAGTATTATTTGCTTAGGTGCCTAAGC
ATCTTTAAAAATTTGATTTAGTTGGCAATACACTGGGAGGTTTCACATAAATATCTAGGT
TTCCAGCTTCTCTGAAGAAATGAGAAAATCCATTTCCACATGGGCACAACCAGCTGGAGC
TAACAGCTGTTAGACAGCATGCTGCTCCCCGCTACTGGCTTACACCCAGCCAGCTTCATG
TGTGCATGTTATCTGCCTGCCATCACATCGGTCTCCCTGCTCAGCAATGTCCCACCTCAC
CTCACACTCATACCCTTATACAGTTCCCTAATCAGATTCAGTAGGCCACCCCACTATAGC
TNGGCATCCCTGGAGCTGGAAATTTGTAACATNTGNTCTTTCACCACGGACCTNCTAGAG
TCAGACTCCTGTGG
Restriction Sites NotI-NotI |
ACCN NM_032795
Insert Size 2640 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032795.1, NP_116184.1
RefSeq Size 2146 bp
RefSeq ORF 1134 bp
Locus ID 84881
UniProt ID Q96CM3
Cytogenetics 11q24.2
Domains PseudoU_synth_2
Summary Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome (PubMed:27974379). Acts on position 39 in mitochondrial tRNA(Phe) (PubMed:28082677). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed:27667664).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
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"NM_032795" in other vectors (4)
SKU Description Size Price
RC204352 RPUSD4 (Myc-DDK-tagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 10 ug
$457.00
RC204352L3 Lenti ORF clone of Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC204352L4 Lenti ORF clone of Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, mGFP tagged 10 ug
$757.00
RG204352 RPUSD4 (tGFP-tagged) - Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 10 ug
$489.00 $657.00

file-alt Citations

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