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SYAP1 (NM_032796) Human Tagged ORF Clone

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SKU
RC204258
SYAP1 (Myc-DDK-tagged)-Human synapse associated protein 1 (SYAP1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SYAP1
Synonyms BSTA; PRO3113
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204258 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGGGGCTTGAGCAGTTGGTTGGGCTTGCAGCAGCCGGTGGCAGGCGGTGGGCAGCCCAATGGAG
ATGCTCTACCCGAGCAGCCGTCCGAGACGGTGGCTGAGTCTGCGGAGGAGGAGCTGCAGCAAGCGGGAGA
CCAGGAGCTCCTCCACCAGGCCAAAGACTTCGGCAACTATTTATTTAACTTTGCATCTGCTGCCACAAAA
AAGATAACTGAATCAGTTGCTGAAACAGCACAAACAATAAAGAAATCCGTAGAAGAAGGAAAAATAGATG
GCATCATTGACAAGACAATTATAGGAGATTTTCAGAAGGAACAGAAAAAATTTGTTGAAGAGCAACATAC
AAAGAAGTCAGAAGCAGCTGTGCCCCCATGGGTTGACACTAACGATGAAGAAACAATTCAACAACAAATT
TTGGCCTTATCAGCTGACAAGAGGAATTTCCTTCGTGACCCTCCGGCTGGCGTGCAATTTAATTTCGACT
TTGATCAGATGTACCCCGTGGCCCTGGTCATGCTCCAGGAGGATGAGCTGCTAAGCAAGATGAGATTTGC
CCTCGTTCCTAAACTTGTGAAGGAAGAAGTGTTCTGGAGGAACTACTTTTACCGCGTCTCCCTGATTAAG
CAGTCAGCCCAGCTCACGGCCCTGGCTGCCCAACAGCAGGCCGCAGGGAAGGAGGAGAAGAGCAATGGCA
GAGAGCAAGATTTGCCGCTGGCAGAGGCAGTACGGCCCAAAACGCCACCCGTTGTAATCAAATCTCAGCT
TAAAACTCAAGAGGATGAGGAAGAAATTTCTACTAGCCCAGGTGTTTCTGAGTTTGTCAGTGATGCCTTC
GATGCCTGTAACCTAAATCAGGAAGATCTAAGGAAAGAAATGGAGCAACTAGTGCTTGACAAAAAGCAAG
AGGAGACAGCCGTACTGGAAGAGGATTCTGCAGATTGGGAAAAAGAACTGCAGCAGGAACTTCAAGAATA
TGAAGTGGTGACAGAATCTGAAAAACGAGATGAAAACTGGGATAAGGAAATAGAGAAAATGCTTCAAGAG
GAAAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204258 protein sequence
Red=Cloning site Green=Tags(s)

MFRGLSSWLGLQQPVAGGGQPNGDALPEQPSETVAESAEEELQQAGDQELLHQAKDFGNYLFNFASAATK
KITESVAETAQTIKKSVEEGKIDGIIDKTIIGDFQKEQKKFVEEQHTKKSEAAVPPWVDTNDEETIQQQI
LALSADKRNFLRDPPAGVQFNFDFDQMYPVALVMLQEDELLSKMRFALVPKLVKEEVFWRNYFYRVSLIK
QSAQLTALAAQQQAAGKEEKSNGREQDLPLAEAVRPKTPPVVIKSQLKTQEDEEEISTSPGVSEFVSDAF
DACNLNQEDLRKEMEQLVLDKKQEETAVLEEDSADWEKELQQELQEYEVVTESEKRDENWDKEIEKMLQE
EN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032796
ORF Size 1056 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032796.4
RefSeq Size 3533 bp
RefSeq ORF 1059 bp
Locus ID 94056
UniProt ID Q96A49
Cytogenetics Xp22.2
Domains BSD
MW 39.9 kDa
Summary Plays a role in adipocyte differentiation by promoting mTORC2-mediated phosphorylation of AKT1 at 'Ser-473' after growth factor stimulation (PubMed:23300339).UniProtKB/Swiss-Prot Function
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Documents
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"NM_032796" in other vectors (4)
SKU Description Size Price
RC204258L3 Lenti ORF clone of Human synapse associated protein 1 (SYAP1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC204258L4 Lenti ORF clone of Human synapse associated protein 1 (SYAP1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG204258 SYAP1 (tGFP-tagged) - Human synapse associated protein 1 (SYAP1), transcript variant 1 10 ug
$489.00 $657.00
SC319647 SYAP1 (untagged)-Human synapse associated protein 1 (SYAP1), transcript variant 1 10 ug
$457.00

Plasmid Map for RC204258

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204258
Circular map for RC204258

file-alt Citations

miR-375 is activated by ASH1 and inhibits YAP1 in a lineage-dependent manner in lung cancer
Nishikawa, Osada, Okazaki et al
Cancer Res. (2011) 71 (19), 6165-73 DOI: 10.1158/0008-5472.CAN-11-1020
Cited by 24
AI Summary: In this study, OriGene’s YAP1 cDNA product was used to investigate the role of YAP1 as a direct target of miR-375 in lung cancer cells.
Snippet: ASH1-expressing lentiviral and plasmid vectors were constructed with CSII-CMV-MCS-IRES2- Blasticidin and pcDNA3 (Invitrogen), respectively, as previously described (9). Yes-associated protein 1 (YAP1) cDNA was purchased from OriGene and inserted into CSII-EF-MCS
Showing 1-1 of 1 papers.
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