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HSPBAP1 (NM_024610) Human Tagged ORF Clone

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SKU
RC204227
HSPBAP1 (Myc-DDK-tagged)-Human HSPB (heat shock 27kDa) associated protein 1 (HSPBAP1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "HSPBAP1 or NM_024610"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HSPBAP1
Synonyms PASS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204227 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAGGCTCCGAGGCGACCACTCCTGTGATCGTTGCGGCTGGGGCTGGAGGGGAGGAAGGTGAAC
ATGTCAAACCTTTTAAGCCAGAGAAAGCAAAAGAAATTATCATGTCTTTACAACAACCTGCAATCTTCTG
TAACATGGTGTTTGATTGGCCAGCACGACACTGGAATGCTAAATACCTTTCGCAGGTCCTTCATGGCAAG
CAGATACGATTCAGAATGGGGATGAAAAGCATGAGCACAGTTCCTCAGTTTGAAACTACATGTAATTACG
TAGAAGCTACACTCGAAGAGTTTCTGACCTGGAACTGTGACCAGTCTAGTATTTCTGGACCATTTAGAGA
TTATGACCATTCCAAGTTCTGGGCTTATGCTGACTATAAATATTTTGTCAGTCTATTTGAAGACAAGACA
GATCTTTTCCAGGATGTGAAATGGTCTGACTTCGGGTTTCCTGGAAGAAATGGACAGGAAAGTACATTGT
GGATTGGCTCCTTGGGAGCCCACACACCCTGTCATCTGGACTCCTATGGTTGTAACTTGGTATTCCAGGT
ACAAGGAAGGAAACGATGGCATCTCTTTCCTCCTGAAGATACTCCTTTCCTTTATCCAACTAGAATCCCT
TATGAAGAATCTAGTGTGTTCAGTAAAATCAATGTTGTCAATCCTGATTTAAAGCGTTTTCCTCAGTTCC
GGAAAGCTCAAAGACATGCGGTTACACTGAGCCCAGGACAGGTTCTCTTTGTTCCCAGACACTGGTGGCA
TTACGTAGAATCCATTGATCCTGTCACTGTCAGTATAAACTCATGGATTGAACTGGAAGAGGATCACCTA
GCCCGGGTAGAAGAGGCAATCACCCGTATGCTTGTGTGTGCCCTGAAAACTGCAGAGAATCCACAAAATA
CCAGAGCCTGGTTAAACCCCACTGAGGTTGAGGAAACGTCCCATGCAGTCAACTGTTGCTACTTAAATGC
AGCTGTTTCTGCATTTTTTGATCGCTGCAGAACATCTGAGGTAGTAGAAATCCAAGCACTGAGAACAGAT
GGAGAGCACATGAAAAAGGAAGAATTAAATGTGTGCAACCACATGGAGGTGGGCCAAACAGGTAGCCAGA
ACTTGACCACAGGAACAGACAAACCGGAGGCAGCAAGTCCCTTTGGCCCTGATCTGGTCCCTGTAGCACA
GAGGTCCGAAGAACCGCCTTCAGAAAGAGGAGGCATATTTGGAAGTGATGGGAAAGACTTTGTGGACAAG
GATGGAGAACACTTTGGAAAATTGCATTGTGCCAAGAGACAACAAATAATGAGCAACAGTGAAAATGCAA
TTGAGGAACAGATTGCCTCAAATACTACTACGACTCCTCAAACATTCATTTCTACGGATGACTTGCTGGA
CTGCTTGGTGAATCCACAAGTAACCAGGATAGTGGCACAACTTTTGATACAAGGAAGAAGTTTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204227 protein sequence
Red=Cloning site Green=Tags(s)

MAAGSEATTPVIVAAGAGGEEGEHVKPFKPEKAKEIIMSLQQPAIFCNMVFDWPARHWNAKYLSQVLHGK
QIRFRMGMKSMSTVPQFETTCNYVEATLEEFLTWNCDQSSISGPFRDYDHSKFWAYADYKYFVSLFEDKT
DLFQDVKWSDFGFPGRNGQESTLWIGSLGAHTPCHLDSYGCNLVFQVQGRKRWHLFPPEDTPFLYPTRIP
YEESSVFSKINVVNPDLKRFPQFRKAQRHAVTLSPGQVLFVPRHWWHYVESIDPVTVSINSWIELEEDHL
ARVEEAITRMLVCALKTAENPQNTRAWLNPTEVEETSHAVNCCYLNAAVSAFFDRCRTSEVVEIQALRTD
GEHMKKEELNVCNHMEVGQTGSQNLTTGTDKPEAASPFGPDLVPVAQRSEEPPSERGGIFGSDGKDFVDK
DGEHFGKLHCAKRQQIMSNSENAIEEQIASNTTTTPQTFISTDDLLDCLVNPQVTRIVAQLLIQGRSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024610
ORF Size 1464 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024610.6
RefSeq Size 1972 bp
RefSeq ORF 1467 bp
Locus ID 79663
UniProt ID Q96EW2, A8K045
Cytogenetics 3q21.1
Domains JmjC
MW 55.2 kDa
Summary This gene encodes a protein that binds to one of the small heat shock proteins, specifically hsp27. Hsp27 is involved with cell growth and differentiation. This encoded protein was found to be abnormally expressed in patients with intractable epilepsy, although how brain function is affected remains unknown. provided by RefSeq, Sep 2011
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"NM_024610" in other vectors (7)
SKU Description Size Price
RC204227L1 Lenti ORF clone of Human HSPB (heat shock 27kDa) associated protein 1 (HSPBAP1), Myc-DDK-tagged 10 ug
$757.00
RC204227L2 Lenti ORF clone of Human HSPB (heat shock 27kDa) associated protein 1 (HSPBAP1), mGFP tagged 10 ug
$757.00
RC204227L3 Lenti ORF clone of Human HSPB (heat shock 27kDa) associated protein 1 (HSPBAP1), Myc-DDK-tagged 10 ug
$757.00
RC204227L4 Lenti ORF clone of Human HSPB (heat shock 27kDa) associated protein 1 (HSPBAP1), mGFP tagged 10 ug
$757.00
RG204227 HSPBAP1 (tGFP-tagged) - Human HSPB (heat shock 27kDa) associated protein 1 (HSPBAP1) 10 ug
$657.00
SC112119 HSPBAP1 (untagged)-Human HSPB (heat shock 27kDa) associated protein 1 (HSPBAP1) 10 ug
$457.00
SC324463 HSPBAP1 (untagged)-Human HSPB (heat shock 27kDa) associated protein 1 (HSPBAP1) 10 ug
$457.00

Plasmid Map for RC204227

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204227
Circular map for RC204227

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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