Syntaxin 5A (STX5) (NM_003164) Human Tagged ORF Clone

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SKU
RC204213
STX5 (Myc-DDK-tagged)-Human syntaxin 5 (STX5)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Syntaxin 5A
Synonyms SED5; STX5A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204213 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCCCGCGGAAACGCTACGGGTCTAAGAACACGGATCAGGGTGTCTACCTGGGTCTCTCAAAGACAC
AGGTCCTGTCCCCTGCAACTGCTGGCAGTAGCAGCAGCGACATCGCCCCTCTGCCCCCCCCAGTGACCCT
CGTCCCTCCCCCTCCCGACACCATGTCCTGCCGGGATCGGACCCAGGAGTTTCTGTCTGCCTGCAAGTCG
CTGCAGACCCGTCAGAATGGAATCCAGACAAATAAGCCAGCTTTGCGTGCTGTCCGACAACGCAGTGAAT
TCACCCTCATGGCCAAGCGCATTGGGAAAGACCTTAGCAACACATTTGCCAAGCTGGAGAAGCTGACAAT
CTTGGCAAAGCGCAAGTCCCTCTTTGATGATAAAGCAGTGGAAATTGAAGAGCTAACATATATCATCAAA
CAGGACATCAATAGCCTCAACAAACAAATTGCTCAGCTCCAGGATTTCGTGAGAGCCAAGGGCAGCCAGA
GTGGCCGGCACCTGCAGACCCACTCCAACACCATTGTGGTCTCCTTGCAGTCGAAACTGGCTTCTATGTC
CAATGACTTCAAATCGGTTTTAGAAGTGAGGACAGAGAACCTGAAGCAGCAGAGGAGCCGGAGAGAGCAG
TTCTCCCGGGCACCTGTGTCAGCCCTGCCCCTTGCCCCTAACCACCTGGGCGGTGGTGCTGTGGTTCTGG
GGGCAGAGTCCCATGCCTCCAAGGATGTCGCCATCGACATGATGGACTCTCGGACCAGCCAGCAGCTGCA
GCTCATTGACGAGCAGGATTCCTACATCCAGAGTCGGGCAGACACCATGCAGAACATTGAGTCGACAATT
GTTGAGTTGGGCTCCATCTTTCAGCAGTTGGCACACATGGTTAAGGAACAGGAGGAAACCATTCAGAGGA
TCGACGAGAACGTGCTAGGAGCCCAGCTGGACGTTGAGGCCGCCCATTCAGAGATCCTCAAGTACTTCCA
GTCTGTCACCTCCAACCGGTGGCTCATGGTCAAAATCTTCCTCATCCTCATTGTCTTCTTCATCATCTTT
GTGGTCTTCCTTGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204213 protein sequence
Red=Cloning site Green=Tags(s)

MIPRKRYGSKNTDQGVYLGLSKTQVLSPATAGSSSSDIAPLPPPVTLVPPPPDTMSCRDRTQEFLSACKS
LQTRQNGIQTNKPALRAVRQRSEFTLMAKRIGKDLSNTFAKLEKLTILAKRKSLFDDKAVEIEELTYIIK
QDINSLNKQIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRSRREQ
FSRAPVSALPLAPNHLGGGAVVLGAESHASKDVAIDMMDSRTSQQLQLIDEQDSYIQSRADTMQNIESTI
VELGSIFQQLAHMVKEQEETIQRIDENVLGAQLDVEAAHSEILKYFQSVTSNRWLMVKIFLILIVFFIIF
VVFLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003164
ORF Size 1065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003164.5
RefSeq Size 1834 bp
RefSeq ORF 1068 bp
Locus ID 6811
UniProt ID Q13190
Cytogenetics 11q12.3
Domains SynN, t_SNARE
Protein Families Druggable Genome, Transmembrane
Protein Pathways SNARE interactions in vesicular transport
MW 39.7 kDa
Summary This gene encodes a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for v-SNAREs (vesicle-SNAP receptors), permitting specific synaptic vesicle docking and fusion. The encoded protein regulates endoplasmic reticulum to Golgi transport and plays a critical role in autophagy. Autoantibodies targeting the encoded protein may be a diagnostic marker for endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Sep 2011
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Documents
Resources
"NM_003164" in other vectors (5)
SKU Description Size Price
RC204213L3 Lenti ORF clone of Human syntaxin 5 (STX5), Myc-DDK-tagged 10 ug
$757.00
RC204213L4 Lenti ORF clone of Human syntaxin 5 (STX5), mGFP tagged 10 ug
$757.00
RG204213 STX5 (tGFP-tagged) - Human syntaxin 5 (STX5) 10 ug
$489.00 $657.00
SC118144 STX5 (untagged)-Human syntaxin 5 (STX5) 10 ug
$300.00
SC317563 STX5 (untagged)-Human syntaxin 5 (STX5) 10 ug
$503.00

Plasmid Map for RC204213

Download sequence for

Cloning scheme for RC204213

Circular map for RC204213

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