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Syntaxin 5A (STX5) (NM_003164) Human Untagged Clone

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SKU
SC118144
STX5 (untagged)-Human syntaxin 5 (STX5)
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Syntaxin 5A
Synonyms SED5; STX5A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_003164 edited
GAATTCGGCACGAGGGTTACGTGTAGGTGACGTGGCCGCTTCCAGTCTTTGGTCGGGTTT
CGGCGGCTTCAGTGCTCGGGGAGGAGGCAGTGACGGCCGGTGAGATTGGAAGTGGCGGCG
GCGGCAGGCGGCAGAGGGGAGTCCGAGGTTATGCGTCTCAATGATCCCGCGGAAACGCTA
CGGGTCTAAGAACACGGATCAGGGTGTCTACCTGGGTCTCTCAAAGACACAGGTCCTGTC
CCCTGCAACTGCTGGCAGTAGCAGCAGCGACATCGCCCCTCTGCCCCCCCCAGTGACCCT
CGTCCCTCCCCCTCCCGACACCATGTCCTGCCGGGATCGGACCCAGGAGTTTCTGTCTGC
CTGCAAGTCGCTGCAGACCCGTCAGAATGGAATCCAGACAAATAAGCCAGCTTTGCGTGC
TGTCCGACAACGCAGTGAATTCACCCTCATGGCCAAGCGCATTGGGAAAGACCTTAGCAA
CACATTTGCCAAGCTGGAGAAGCTGACAATCTTGGCAAAGCGCAAGTCCCTCTTTGATGA
TAAAGCAGTGGAAATTGAAGAGCTAACATATATCATCAAACAGGACATCAATAGCCTCAA
CAAACAAATTGCTCAGCTCCAGGATTTCGTGAGAGCCAAGGGCAGCCAGAGTGGCCGGCA
CCTGCAGACCCACTCCAACACCATTGTGGTCTCCTTGCAGTCGAAACTGGCTTCTATGTC
CAATGACTTCAAATCGGTTTTAGAAGTGAGGACAGAGAACCTGAAGCAGCAGAGGAGCCG
GAGAGAGCAGTTCTCCCGGGCACCTGTGTCAGCCCTGCCCCTTGCCCCTAACCACCTGGG
CGGTGGTGCTGTGGTTCTGGGGGCAGAGTCCCATGCCTCCAAGGATGTCGCCATCGACAT
GATGGACTCTCGGACCAGCCAGCAGCTGCAGCTCATTGACGAGCAGGATTCCTACATCCA
GAGTCGGGCAGACACCATGCAGAACATTGAGTCGACAATTGTTGAGTTGGGCTCCATCTT
TCAGCAGTTGGCACACATGGTTAAGGAACAGGAGGAAACCATTCAGAGGATCGACGAGAA
CGTGCTAGGAGCCCAGCTGGACGTTGAGGCCGCCCATTCAGAGATCCTCAAGTACTTCCA
GTCTGTCACCTCCAACCGGTGGCTCATGGTCAAAATCTTCCTCATCCTCATTGTCTTCTT
CATCATCTTTGTGGTCTTCCTTGCTTGAACCCTCTCTACTCTGAGGCACTCTGTTGGGGT
TTGGGACCCTCCTGGGAAGGCAAGTGGCCAGTGCTGCCACTGAGCCTGTGCAGGGTACTT
GGGAGAAAGGCCCTGTTTCCCTGGAACTGCTAAGAATGACCACTGCCCCTGATCCCCCAC
CCCTTGCCTCTGGCCACCCTGTCCTCCCCCCACCACCCTCAGGCCTATGAAACACACAGG
GTTCTAGATTTGAACTCTGCTGTGAAGTGACTGGAAGGGAGCAGAGGCCAGCTGGGGGCC
AGTGGGGGAGGTTGTTTCCACTAGGAGATTTTTATAAACCCTCTCCAGCCTCTCCCAAAG
GAAGCGTTGGCAGCAAAGGGAGATGATGCCCTTACCCACCTTCCTGTGAGTGAAGAGAGG
AAGCAGCCCCAGGGACCAATTTTCCCAATTGACCTCTTTCTTCCTCTTTCACCATGTGAG
GCAGGGAGCCCTGAGCCCTTCAGCTGCCTGCACAACCCCTGACATTGGCTGCTGGTGACT
CAATCTGCCAAATGTGCTGCAGCTCGTTTTCTCCCAATTACAGCAAGACTGTCAGCCTCA
AAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003164 unedited
NAATACGTTCGGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGTTA
CGTGTAGGTGACGTGGCCGCTTCCAGTCTTTGGTCGGGTTTCGGCGGCTTCAGTGCTCGG
GGAGGAGGCAGTGACGGCCGGTGAGATTGGAAGTGGCGGCGGCGGCAGGCGGCAGAGGGG
AGTCCGAGGTTATGCGTCTCAATGATCCCGCGGAAACGCTACGGGTCTAAGAACACGGAT
CAGGGTGTCTACCTGGGTCTCTCAAAGACACAGGTCCTGTCCCCTGCAACTGCTGGCAGT
AGCAGCAGCGACATCGCCCCTCTGCCCCCCCCAGTGACCCTCGTCCCTCCCCCTCCCGAC
ACCATGTCCTGCCGGGATCGGACCCAGGAGTTTCTGTCTGCCTGCAAGTCGCTGCAGACC
CGTCAGAATGGAATCCAGACAAATAAGCCAGCTTTGCGTGCTGTCCGACAACGCAGTGAA
TTCACCCTCATGGCCAAGCGCATTGGGAAAGACCTTAGCAACACATTTGCCAAGCTGGAG
AAGCTGACAATCTTGGCAAAGCGCAAGTCCCTCTTTGATGATAAAGCAGTGGAAATTGAA
GAGCTAACATATATCATCAAACAGGACATCAATAGCCTCAACAAACAAATTGCTCAGCTC
CAGGATTTCGTGAGAGCCAAGGGCAGCCAGAGTGGCCGGCACCTGCAGACCCACTCCAAC
ACCATTGTGGTCTCCTTGCAGTCGAAACTGGCTTCTATGTCCAATGACTTCAAATCGGTT
TTAGAAGTGAGGACAGAGAACCTGAAGCAGCAGAAGAGCCGGAGAGAGCAGTTCTCCCGG
GCACCTGTGTCAGCCCTGCCCCTTGCCCCTAACCACCTGGGGCGGTGGTGCTGTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003164 unedited
GCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTGAGGCTGACAGTCTTGCTGTAA
TTGGGAGAAAACGAGCTGCAGCACATTTGGCAGATTGAGTCACCAGCAGCCAATGTCAGG
GGTTGTGCAAGCAGCTGAAGGGCTCAAGGCTCCCTGCCTCACATGGTGAAAGAGGAAGAA
AGAGGTCAATTGGGAAAATTGGTCCCTGGGGCTGCTTCCTCTCTTCACTCACAGGAAGGT
GGGTAAGGGCATCATCTCCCTTTGCTGCCAACGCTTCCTTTGGGAGAGGCTGGAGAGGGT
TTATAAAAATCTCCTAGTGGAAACAACCTCCCCCACTGGCCCCCAGCTGGCCTCTGCTCC
CTTCCAGTCACTTCACAGCAGAGTTCAAATCTAGAACCCTGTGTGTTTCATATGCCTGAG
GGTGGTGGGGGGAGGACAGGGTGGCCATAGGCAAGGGGTGGGGGATCAGGGGCATTGGTC
ATTTCTTACCATTCCAGGGAAACAGGCCTTTCTCCAATTCCCTGCCAGGCTCAGGGGATC
ACTGGCCCTTGCCTTCCAGATGGTCCCAACCCCACATACGCCTAAATCTCACAGGTTCAC
GCAGGAAACCTCTTGAGCTGATAATACTTGAGATGAGAACATTTGACCTGACCCCCCGGT
GGAGGCGCTCCGGATCACTTAGGTCTCTGACGGCGGCCACCCTTCACCGGACTTCTCTCC
CTTCCCCCTACCTTCTTGCCCTCACTAAACTTACCTGCTCACCTTCTCTTCATTCTCTTC
ACCCCTATTTATATTCCTTCCTCTCTAACCCTCACAACAATCCCCACCTCACATATCATC
TAGAATTTCCTTCCGGCTCCACCACCTCCACCTCTCCTCACTACATATTCTTATTTACTC
TCCCCCCCCT
Restriction Sites NotI-NotI |
ACCN NM_003164
Insert Size 1830 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003164.2, NP_003155.1
RefSeq Size 1792 bp
RefSeq ORF 906 bp
Locus ID 6811
UniProt ID Q13190
Cytogenetics 11q12.3
Domains SynN, t_SNARE
Protein Families Druggable Genome, Transmembrane
Protein Pathways SNARE interactions in vesicular transport
Summary This gene encodes a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for v-SNAREs (vesicle-SNAP receptors), permitting specific synaptic vesicle docking and fusion. The encoded protein regulates endoplasmic reticulum to Golgi transport and plays a critical role in autophagy. Autoantibodies targeting the encoded protein may be a diagnostic marker for endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Sep 2011
Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_003164" in other vectors (5)
SKU Description Size Price
RC204213 STX5 (Myc-DDK-tagged)-Human syntaxin 5 (STX5) 10 ug
$457.00
RC204213L3 Lenti ORF clone of Human syntaxin 5 (STX5), Myc-DDK-tagged 10 ug
$757.00
RC204213L4 Lenti ORF clone of Human syntaxin 5 (STX5), mGFP tagged 10 ug
$757.00
RG204213 STX5 (tGFP-tagged) - Human syntaxin 5 (STX5) 10 ug
$489.00 $657.00
SC317563 STX5 (untagged)-Human syntaxin 5 (STX5) 10 ug
$503.00

file-alt Citations

COPII vesicles can affect the activity of antisense oligonucleotides by facilitating the release of oligonucleotides from endocytic pathways
Liang, Sun, Nichols et al
Nucleic Acids Res. (2018)
AI Summary: OriGene products, including the Sar1a (RC201450), SEC13 (SC111014) plasmids, and the STX5 (SC118144) cDNA clone, were used as templates for PCR amplification to study the roles of these proteins in COPII vesicle-mediated release of antisense oligonucleotides from endocytic pathways.
Snippet: Promega). Briefly, Sar1a and SEC13 were amplified from the plasmids RC201450 and SC111014 (Origene) using PCR BioLabs). STX5 (NM 003164) was amplified by PCR from the full-length complementary DNA clone (Origene SC118144)
Showing 1-1 of 1 papers.
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