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PTCD3 (NM_017952) Human Tagged ORF Clone

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SKU
RC204119
PTCD3 (Myc-DDK-tagged)-Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $631.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PTCD3
Synonyms MRP-S39
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204119 representing NM_017952
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTTGTATCTGCTGTTCGCTGGCTGGGCCTCCGCAGCAGGCTTGGCCAGCCGCTGACGGGTCGGC
GGGCGGGTTTGTGTGAACAGGCACGCAGCTGCAGATTTTATTCTGGTAGTGCAACCCTCTCAAAGGTTGA
AGGAACTGATGTAACAGGGATTGAAGAAGTAGTAATTCCAAAAAAGAAAACTTGGGATAAAGTAGCCGTT
CTTCAGGCACTTGCATCCACAGTAAACAGGGATACCACAGCTGTGCCTTATGTGTTTCAAGATGATCCTT
ACCTTATGCCAGCATCATCTTTGGAATCTCGTTCATTTTTACTGGCAAAGAAATCCGGGGAGAATGTGGC
CAAGTTTATTATTAATTCATACCCCAAATATTTTCAGAAGGACATAGCTGAACCTCATATACCGTGTTTA
ATGCCTGAGTACTTTGAACCTCAGATCAAAGACATAAGTGAAGCCGCCCTGAAGGAACGAATTGAGCTCA
GAAAAGTCAAAGCCTCTGTGGACATGTTTGATCAGCTTTTGCAAGCAGGAACCACTGTGTCTCTTGAAAC
AACAAATAGTCTCTTGGATTTATTGTGTTACTATGGTGACCAGGAGCCCTCAACTGATTACCATTTTCAA
CAAACTGGACAGTCAGAAGCATTGGAAGAGGAAAATGATGAGACATCTAGGAGGAAAGCTGGTCATCAGT
TTGGAGTTACATGGCGAGCAAAAAACAACGCTGAGAGAATCTTTTCTCTAATGCCAGAGAAAAATGAACA
TTCCTATTGCACAATGATCCGAGGAATGGTGAAGCACCGAGCTTATGAGCAGGCATTAAACTTGTACACT
GAGTTACTAAACAACAGACTCCATGCTGATGTATACACATTTAATGCATTGATTGAAGCAACAGTATGTG
CGATAAATGAGAAATTTGAGGAAAAATGGAGTAAAATACTGGAGCTGCTAAGACACATGGTTGCACAGAA
GGTGAAACCAAATCTTCAGACTTTTAATACCATTCTGAAATGTCTCCGAAGATTTCATGTGTTTGCAAGA
TCGCCAGCCTTACAGGTTTTACGTGAAATGAAAGCCATTGGAATAGAACCCTCGCTTGCAACATATCACC
ATATTATTCGCCTGTTTGATCAACCTGGAGACCCTTTAAAGAGATCATCCTTCATCATTTATGATATAAT
GAATGAATTAATGGGAAAGAGATTTTCTCCAAAGGACCCGGATGATGATAAGTTTTTTCAGTCAGCCATG
AGCATATGCTCATCTCTCAGAGATCTAGAACTTGCCTACCAAGTACATGGCCTTTTAAAAACCGGAGACA
ACTGGAAATTCATTGGACCTGATCAACATCGTAATTTCTATTATTCCAAGTTCTTCGATTTGATTTGTCT
AATGGAACAAATTGATGTTACCTTGAAGTGGTATGAGGACCTGATACCTTCAGCCTACTTTCCCCACTCC
CAAACAATGATACATCTTCTCCAAGCATTGGATGTGGCCAATCGGCTAGAAGTGATTCCTAAAATTTGGA
AAGATAGTAAAGAATATGGTCATACTTTCCGCAGTGACCTGAGAGAAGAGATCCTGATGCTCATGGCAAG
GGACAAGCACCCACCAGAGCTTCAGGTGGCATTTGCTGACTGTGCTGCTGATATCAAATCTGCGTATGAA
AGCCAACCCATCAGACAGACTGCTCAGGATTGGCCAGCCACCTCTCTCAACTGTATAGCTATCCTCTTTT
TAAGGGCTGGGAGAACTCAGGAAGCCTGGAAAATGTTGGGGCTTTTCAGGAAGCATAATAAGATTCCTAG
AAGTGAGTTGCTGAATGAGCTTATGGACAGTGCAAAAGTGTCTAACAGCCCTTCCCAGGCCATTGAAGTA
GTAGAGCTGGCAAGTGCCTTCAGCTTACCTATTTGTGAGGGCCTCACCCAGAGAGTAATGAGTGATTTTG
CAATCAACCAGGAACAAAAGGAAGCCCTAAGTAATCTAACTGCATTGACCAGTGACAGTGATACTGACAG
CAGCAGTGACAGCGACAGTGACACCAGTGAAGGCAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204119 representing NM_017952
Red=Cloning site Green=Tags(s)

MAVVSAVRWLGLRSRLGQPLTGRRAGLCEQARSCRFYSGSATLSKVEGTDVTGIEEVVIPKKKTWDKVAV
LQALASTVNRDTTAVPYVFQDDPYLMPASSLESRSFLLAKKSGENVAKFIINSYPKYFQKDIAEPHIPCL
MPEYFEPQIKDISEAALKERIELRKVKASVDMFDQLLQAGTTVSLETTNSLLDLLCYYGDQEPSTDYHFQ
QTGQSEALEEENDETSRRKAGHQFGVTWRAKNNAERIFSLMPEKNEHSYCTMIRGMVKHRAYEQALNLYT
ELLNNRLHADVYTFNALIEATVCAINEKFEEKWSKILELLRHMVAQKVKPNLQTFNTILKCLRRFHVFAR
SPALQVLREMKAIGIEPSLATYHHIIRLFDQPGDPLKRSSFIIYDIMNELMGKRFSPKDPDDDKFFQSAM
SICSSLRDLELAYQVHGLLKTGDNWKFIGPDQHRNFYYSKFFDLICLMEQIDVTLKWYEDLIPSAYFPHS
QTMIHLLQALDVANRLEVIPKIWKDSKEYGHTFRSDLREEILMLMARDKHPPELQVAFADCAADIKSAYE
SQPIRQTAQDWPATSLNCIAILFLRAGRTQEAWKMLGLFRKHNKIPRSELLNELMDSAKVSNSPSQAIEV
VELASAFSLPICEGLTQRVMSDFAINQEQKEALSNLTALTSDSDTDSSSDSDSDTSEGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017952
ORF Size 2067 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017952.6
RefSeq Size 6708 bp
RefSeq ORF 2070 bp
Locus ID 55037
UniProt ID Q96EY7
Cytogenetics 2p11.2
Domains PPR
MW 78.4 kDa
Summary Mitochondrial RNA-binding protein that has a role in mitochondrial translation.UniProtKB/Swiss-Prot Function
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"NM_017952" in other vectors (7)
SKU Description Size Price
RC204119L1 Lenti ORF clone of Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$931.00
RC204119L2 Lenti ORF clone of Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$931.00
RC204119L3 Lenti ORF clone of Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$931.00
RC204119L4 Lenti ORF clone of Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$931.00
RG204119 PTCD3 (tGFP-tagged) - Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein 10 ug
$789.00 $831.00
SC113866 PTCD3 (untagged)-Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein 10 ug
$512.00
SC318164 PTCD3 (untagged)-Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein 10 ug
$695.00

Plasmid Map for RC204119

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204119
Circular map for RC204119

file-alt Citations

Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components
Alabert, Bukowski-Wills, Lee et al
Nat. Cell Biol. (2014) 16 (3), 281-93 DOI: 10.1038/ncb2918
Cited by 372
AI Summary: OriGene products contributed to this study by providing plasmids (FAM111A–tGFP [RG210012], FAM178A–FLAG [RC227146], PTCD3–FLAG [RC204119], RPL29–FLAG [RC203616], KIAA0391–FLAG [RC206805], MPST–FLAG [RC202466]) and siRNAs targeting FAM111A, which were used for protein expression and gene silencing experiments to investigate chromatin dynamics during DNA replication.
Snippet: The FAM111A–tGFP (RG210012), FAM178A–FLAG (RC227146), PTCD3–FLAG (RC204119), RPL29–FLAG (RC203616), KIAA0391–FLAG (RC206805) and MPST–FLAG (RC202466) plasmids were from OriGene. The FAM111A PIP mutant was generated by site-directed mutagenesis of Y24A and F25A, and the FAM178A APIM mutant was generated by site-directed mutagenesis of Y142A. …siRNAs against FAM111A were 5′-GGAAGAUAACCACAUAUUUGGCAGG-3′ (FAM111A siRNA1, OriGene) and 5′-AGAGCUAAAUGCUUGAUUAGAAATG-3′(FAM111A siRNA2, OriGene)
Showing 1-1 of 1 papers.
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