SLC25A36 (NM_018155) Human Tagged ORF Clone

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SKU
RC204111
SLC25A36 (Myc-DDK-tagged)-Human solute carrier family 25, member 36 (SLC25A36), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC25A36
Synonyms PNC2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204111 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCAGAGGGACACGCTGGTGCATCTGTTTGCCGGAGGATGTGGTGGTACAGTGGGAGCTATTCTGA
CATGTCCACTGGAAGTTGTAAAAACACGACTGCAGTCATCTTCTGTGACGCTTTATATTTCTGAAGTTCA
GCTGAACACCATGGCTGGAGCCAGTGTCAACCGAGTAGTGTCTCCCGGACCTCTTCATTGCCTAAAGGTG
ATCTTGGAAAAAGAAGGGCCTCGTTCCTTGTTTAGAGGACTAGGCCCCAATTTAGTGGGGGTAGCCCCTT
CCAGAGCAATATACTTTGCTGCTTATTCAAACTGCAAGGAAAAGTTGAATGATGTATTTGATCCTGATTC
TACCCAAGTACATATGATTTCAGCTGCAATGGCAGGTTTTACTGCAATCACAGCAACCAACCCCATTTGG
CTTATAAAGACTCGGTTACAGCTTGATGCAAGGAACCGCGGGGAAAGGCGAATGGGTGCTTTTGAATGTG
TTCGTAAAGTGTATCAGACAGATGGACTAAAAGGATTTTATAGGGGCATGTCTGCTTCATATGCTGGTAT
ATCAGAGACTGTTATCCATTTTGTTATTTATGAAAGTATAAAACAAAAACTACTGGAATATAAGACTGCT
TCTACAATGGAAAATGATGAAGAGTCTGTGAAAGAAGCATCAGATTTTGTGGGAATGATGCTAGCTGCTG
CCACCTCAAAAACTTGTGCCACAACTATAGCATATCCACATGAAGTTGTAAGAACAAGACTACGTGAAGA
GGGAACAAAATACAGATCTTTTTTTCAGACTCTATCTTTGCTTGTTCAAGAAGAAGGTTATGGGTCTCTT
TATCGTGGTCTGACAACTCATCTAGTGAGACAGATTCCAAACACAGCCATTATGATGGCCACCTATGAAT
TGGTGGTTTACCTACTCAATGGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204111 protein sequence
Red=Cloning site Green=Tags(s)

MSQRDTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSSSVTLYISEVQLNTMAGASVNRVVSPGPLHCLKV
ILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAYSNCKEKLNDVFDPDSTQVHMISAAMAGFTAITATNPIW
LIKTRLQLDARNRGERRMGAFECVRKVYQTDGLKGFYRGMSASYAGISETVIHFVIYESIKQKLLEYKTA
STMENDEESVKEASDFVGMMLAAATSKTCATTIAYPHEVVRTRLREEGTKYRSFFQTLSLLVQEEGYGSL
YRGLTTHLVRQIPNTAIMMATYELVVYLLNG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018155
ORF Size 933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018155.3
RefSeq Size 4674 bp
RefSeq ORF 933 bp
Locus ID 55186
UniProt ID Q96CQ1
Cytogenetics 3q23
Domains mito_carr
Protein Families Druggable Genome, Transmembrane
MW 34.3 kDa
Summary Mitochondrial transporter that imports/exports pyrimidine nucleotides into and from mitochondria. Transports preferentially cytosine and uracil (deoxy)nucleoside mono-, di-, and triphosphates by uniport and antiport mechanism. Also transports guanine but not adenine (deoxy)nucleotides. Is inhibited strongly by pyridoxal 5'-phosphate, 4,7-diphenyl-1,10-phenanthroline, tannic acid, and mercurials (mercury dichloride, Mersalyl acid, p-hydroxymercuribenzoate). Participates in mitochondrial genome maintenance, regulation of mitochondrial membrane potential and mitochondrial respiration.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_018155" in other vectors (4)
SKU Description Size Price
RC204111L3 Lenti ORF clone of Human solute carrier family 25, member 36 (SLC25A36), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC204111L4 Lenti ORF clone of Human solute carrier family 25, member 36 (SLC25A36), transcript variant 2, mGFP tagged 10 ug
$600.00
RG204111 SLC25A36 (tGFP-tagged) - Human solute carrier family 25, member 36 (SLC25A36), transcript variant 2 10 ug
$500.00
SC113721 SLC25A36 (untagged)-Human solute carrier family 25, member 36 (SLC25A36), transcript variant 2 10 ug
$300.00

Plasmid Map for RC204111

Download sequence for

Cloning scheme for RC204111

Circular map for RC204111

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