CD99 (NM_002414) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CD99 |
| Synonyms | HBA71; MIC2; MIC2X; MIC2Y; MSK5X |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002414 |
| ORF Size | 555 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002414.5 |
| RefSeq Size | 1255 bp |
| RefSeq ORF | 558 bp |
| Locus ID | 4267 |
| UniProt ID | P14209 |
| Cytogenetics | X;Y |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration |
| MW | 18.8 kDa |
| Summary | The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. provided by RefSeq, Mar 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002414" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204056L1 | Lenti ORF clone of Human CD99 molecule (CD99), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC204056L2 | Lenti ORF clone of Human CD99 molecule (CD99), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RC204056L3 | Lenti ORF clone of Human CD99 molecule (CD99), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC204056L4 | Lenti ORF clone of Human CD99 molecule (CD99), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG204056 | CD99 (tGFP-tagged) - Human CD99 molecule (CD99), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC118640 | CD99 (untagged)-Human CD99 molecule (CD99), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity
Hammerschmidt, Ostkotte, Nolte et al
Cell (2019) 177 (6), 1536-1552.e23 DOI: 10.1016/j.cell.2019.05.008
Hammerschmidt, Ostkotte, Nolte et al
Cell (2019) 177 (6), 1536-1552.e23 DOI: 10.1016/j.cell.2019.05.008
Cancer-associated mutations in the canonical cleavage site do not influence CD99 shedding by the metalloprotease meprin β but alter cell migration in vitro
Bedau, Schumacher, Peters et al
Oncotarget (2017) 8 (33), 54873-54888 DOI: 10.18632/oncotarget.18966
Bedau, Schumacher, Peters et al
Oncotarget (2017) 8 (33), 54873-54888 DOI: 10.18632/oncotarget.18966
Snippet: Cells were transiently transfected at 60% confluency using polyethylenimine (PEI) and the following expression constructs: empty vector control (pcDNA3.1), human meprin β (both pcDNA3.1, kindly provided by Erwin Sterchi), human CD99 WT (pCMV6 myc-/FLAG-tagged, Origene, #RC204056), and D92H and D92Y (self-mutated from hCD99 WT construct)
Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration
Bedau, Peters, Prox et al
FASEB J. (2016)
Bedau, Peters, Prox et al
FASEB J. (2016)
Showing 1-3 of 3 papers.
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