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EIF4H (NM_031992) Human Tagged ORF Clone

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SKU
RC204028
EIF4H (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF4H
Synonyms eIF-4H; WBSCR1; WSCR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204028 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACTTCGACACCTACGACGATCGGGCCTACAGCAGCTTCGGCGGCGGCAGAGGGTCCCGCGGCA
GTGCTGGTGGCCATGGTTCCCGTAGCCAGAAGGAGTTGCCCACAGAGCCCCCCTACACAGCATACGTAGG
AAATCTACCTTTCAATACGGTTCAGGGCGACATAGATGCTATCTTTAAGGATCTCAGCATAAGGAGTGTA
CGGCTAGTCAGAGACAAAGACACAGATAAATTTAAAGGATTCTGCTATGTAGAATTCGATGAAGTGGATT
CCCTTAAGGAAGCCTTGACATACGATGGTGCACTGTTGGGCGATCGGTCACTTCGTGTGGACATTGCAGA
AGGCAGAAAACAAGATAAAGGTGGCTTTGGATTCAGAAAAGGTGGACCAGATGACAGAGGCTTCAGGGAT
GACTTCTTAGGGGGCAGGGGAGGTAGTCGCCCAGGCGACCGGCGAACAGGCCCCCCCATGGGCAGCCGCT
TCAGAGATGGCCCTCCCCTCCGTGGATCCAACATGGATTTCAGAGAACCCACAGAAGAGGAAAGAGCACA
GAGACCACGACTCCAGCTTAAACCTCGAACAGTCGCGACGCCCCTCAATCAAGTAGCCAATCCCAACTCT
GCTATCTTCGGGGGTGCCAGGCCTAGAGAGGAAGTCGTTCAAAAGGAGCAAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204028 protein sequence
Red=Cloning site Green=Tags(s)

MADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKDLSIRSV
RLVRDKDTDKFKGFCYVEFDEVDSLKEALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKGGPDDRGFRD
DFLGGRGGSRPGDRRTGPPMGSRFRDGPPLRGSNMDFREPTEEERAQRPRLQLKPRTVATPLNQVANPNS
AIFGGARPREEVVQKEQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031992
ORF Size 684 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031992.2
RefSeq Size 2486 bp
RefSeq ORF 687 bp
Locus ID 7458
UniProt ID Q15056
Cytogenetics 7q11.23
Domains RRM
MW 25.2 kDa
Summary This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_031992" in other vectors (4)
SKU Description Size Price
RC204028L3 Lenti ORF clone of Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC204028L4 Lenti ORF clone of Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 2, mGFP tagged 10 ug
$600.00
RG204028 EIF4H (tGFP-tagged) - Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 2 10 ug
$489.00 $500.00
SC109884 EIF4H (untagged)-Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 2 10 ug
$300.00

Plasmid Map for RC204028

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204028
Circular map for RC204028

file-alt Citations

Targetome analysis of chaperone-mediated autophagy in cancer cells
Hao, Kacal, Ouchida et al
Autophagy (2019) 15 (9), 1558-1571 DOI: 10.1080/15548627.2019.1586255
AI Summary: OriGene plasmids pCMV6-DDX3 (RC204171), pCMV6-EIF4A1 (RC203298), and pCMV6-EIF4H (RC204028) were used to overexpress target proteins for functional analysis of chaperone-mediated autophagy substrates in cancer cells.
Snippet: Plasmids were purchased from OriGene: pCMV6-DDX3 (RC204171), pCMV6-EIF4A1 (RC203298), pCMV6-EIF4H (RC204028). Transfections were performed using Viafect (Promega, E4982) according to the manufacturer’s instructions
Showing 1-1 of 1 papers.
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