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PHLPP (PHLPP1) (NM_194449) Human Tagged ORF Clone

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SKU
RC203930
PHLPP1 (Myc-DDK-tagged)-Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,049.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "PHLPP or NM_194449"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHLPP
Synonyms PHLPP; PLEKHE1; PPM3A; SCOP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203930 representing NM_194449
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCGGAGATTGGCTGCCTCATCCGCTTCTATGCAGGAAAACCTCACAGCACGGGTAGCTCTGAAC
GGATTCAGCTCTCAGGAATGTATAATGTCCGTAAAGGCAAGATGCAGTTGCCAGTGAACCGATGGACAAG
ACGCCAAGTCATCCTATGTGGGACCTGCCTGATAGTATCATCTGTGAAAGACAGCTTGACCGGAAAGATG
CATGTTCTGCCACTAATTGGTGGAAAAGTAGAAGAAGTGAAAAAGCACCAACACTGTTTAGCATTTAGCT
CCTCTGGACCCCAAAGCCAGACTTACTACATTTGCTTTGATACTTTCACAGAATACTTAAGGTGGCTGCG
ACAAGTCTCCAAGGTTGCATCCCAGCGCATTAGCTCAGTGGACCTCTCGTGTTGTAGCCTGGAACATCTG
CCTGCCAACCTCTTCTACAGCCAAGACCTCACTCATCTCAATTTAAAACAAAACTTCCTAAGGCAGAACC
CTAGCCTTCCAGCTGCCAGGGGGCTTAATGAACTGCAAAGGTTCACCAAGTTGAAGAGTCTTAACCTTTC
CAATAATCATTTAGGGGACTTCCCTCTGGCAGTCTGCAGTATTCCAACCCTGGCAGAGCTGAACGTGTCC
TGCAATGCCCTGCGATCAGTCCCGGCAGCCGTTGGAGTGATGCACAACTTACAGACATTTTTGTTGGATG
GAAACTTTCTCCAATCCCTTCCTGCTGAGTTGGAGAACATGAAGCAGCTTAGTTATCTGGGTCTTTCTTT
CAATGAATTTACTGACATTCCCGAAGTATTGGAGAAATTGACTGCTGTGGATAAACTTTGTATGTCTGGA
AACTGTGTGGAGACCCTTAGGCTACAGGCTTTAAGAAAAATGCCTCACATTAAACATGTGGATCTAAGGT
TGAACGTAATTAGGAAGCTGATAGCAGATGAAGTGGACTTTCTACAGCATGTTACTCAGCTTGACCTACG
AGACAATAAGCTTGGTGATCTAGATGCTATGATTTTCAACAACATTGAAGTTTTACACTGTGAAAGGAAT
CAACTGGTCACATTAGACATCTGTGGCTATTTCCTAAAAGCGCTCTATGCCTCTTCTAATGAACTTGTTC
AACTTGATGTTTACCCAGTTCCAAATTATCTGTCCTACATGGATGTTTCAAGGAACCGCTTAGAAAATGT
GCCTGAGTGGGTATGTGAAAGCCGAAAGCTAGAAGTTTTGGATATTGGCCATAATCAAATATGTGAACTT
CCTGCCCGCTTATTTTGTAATAGCAGTCTCCGGAAACTACTGGCAGGACACAACCAGTTGGCAAGGCTGC
CTGAAAGGCTAGAAAGAACCTCGGTGGAGGTCTTGGATGTGCAACACAACCAGCTCCTTGAGCTCCCACC
TAACCTTCTGATGAAGGCTGACAGCCTGAGATTCCTGAACGCCTCTGCGAACAAACTGGAAAGCCTTCCT
CCAGCCACGCTTTCCGAAGAGACAAACAGTATCTTACAAGAGTTGTATTTGACAAATAACAGCCTCACAG
ACAAATGTGTGCCCTTGTTAACGGGACACCCCCATTTGAAGATCCTTCACATGGCCTATAACCGACTTCA
GAGTTTTCCAGCAAGTAAAATGGCGAAACTGGAGGAACTTGAAGAAATTGATCTCAGTGGGAATAAGCTG
AAAGCCATCCCAACAACGATCATGAATTGCAGGCGCATGCACACCGTGATTGCTCACTCCAACTGCATCG
AGGTCTTTCCCGAAGTTATGCAGCTCCCAGAGATCAAGTGTGTGGACCTGAGCTGTAATGAGCTAAGTGA
AGTCACATTACCAGAAAACCTGCCTCCCAAACTGCAGGAGCTAGACCTGACTGGAAACCCGCGCCTTGTC
CTTGATCACAAAACCCTGGAACTACTGAATAATATCCGCTGTTTCAAGATTGATCAGCCTTCTACAGGAG
ACGCTTCCGGAGCCCCAGCTGTATGGAGTCATGGTTACACTGAAGCTTCGGGGGTAAAAAACAAGTTGTG
TGTCGCAGCCCTGTCGGTGAATAACTTCTGTGACAACCGCGAAGCCCTGTATGGTGTGTTTGACGGAGAC
CGGAATGTGGAGGTGCCCTACCTTCTCCAGTGCACTATGAGTGACATTTTGGCTGAAGAGCTGCAAAAAA
CAAAAAACGAAGAAGAATACATGGTCAATACATTCATTGTCATGCAAAGGAAACTTGGAACTGCTGGGCA
GAAGCTTGGTGGTGCCGCTGTCCTTTGTCATATCAAGCATGACCCTGTGGATCCAGGAGGATCCTTCACC
TTGACCTCTGCTAATGTGGGCAAGTGCCAAACAGTTCTCTGTCGAAATGGAAAGCCGCTGCCTCTGTCCA
GATCTTACATCATGAGCTGTGAAGAAGAGCTGAAGAGGATTAAACAGCACAAGGCCATTATCACTGAGGA
TGGCAAGGTGAACGGAGTGACTGAGTCCACGCGCATCCTGGGCTACACCTTCCTCCATCCCAGTGTGGTG
CCTCGCCCCCACGTGCAGTCCGTGCTCCTGACTCCCCAGGATGAGTTCTTCATCCTAGGCAGTAAGGGGT
TGTGGGACAGCCTGTCCGTCGAGGAGGCCGTGGAAGCCGTGCGCAACGTGCCCGATGCCCTGGCTGCTGC
CAAGAAGCTGTGTACCCTGGCCCAGAGCTACGGCTGCCACGACAGCATCAGCGCTGTGGTGGTGCAGCTC
AGTGTCACTGAGGACAGCTTCTGCTGCTGCGAGCTCAGCGCCGGTGGGGCTGTGCCACCACCCAGTCCTG
GCATCTTTCCTCCCTCAGTGAACATGGTGATCAAGGATCGGCCCTCAGATGGGCTGGGCGTGCCGTCCTC
CAGCAGCGGCATGGCTTCCGAGATTAGCAGTGAGCTCTCCACTTCTGAGATGAGCAGCGAGGTGGGGTCA
ACAGCCTCCGATGAGCCCCCGCCCGGAGCCCTAAGCGAGAACAGCCCTGCCTACCCCAGTGAGCAGCGCT
GCATGCTCCACCCCATCTGTCTGTCCAACTCCTTCCAGCGCCAGCTATCCAGCGCCACGTTCTCTAGCGC
CTTCTCCGACAACGGCCTTGACAGTGACGATGAGGAGCCCATCGAGGGCGTCTTCACCAACGGCAGCCGG
GTGGAGGTGGAGGTGGACATCCACTGCAGCCGGGCCAAGGAGAAGGAGAAACAGCAGCACCTGCTTCAGG
TGCCAGCAGAGGCCAGTGATGAGGGCATTGTCATCAGCGCCAACGAGGATGAGCCAGGTCTGCCCAGGAA
GGCAGACTTCTCTGCCGTTGGGACCATTGGGCGCCGGAGGGCCAATGGCTCTGTTGCGCCCCAGGAAAGG
AGCCACAATGTGATAGAGGTGGCTACAGACGCACCTCTTCGAAAGCCTGGAGGCTATTTTGCTGCCCCGG
CTCAGCCGGATCCTGATGATCAGTTTATCATACCCCCGGAGCTGGAAGAGGAGGTCAAAGAAATCATGAA
GCATCACCAGGAGCAACAGCAGCAGCAGCAGCCGCCACCACCCCCTCAGCTCCAGCCGCAGCTGCCGCGG
CACTACCAGCTGGACCAGCTGCCAGATTATTACGACACGCCACTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203930 representing NM_194449
Red=Cloning site Green=Tags(s)

MDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKM
HVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHL
PANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVS
CNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSG
NCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERN
QLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICEL
PARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLP
PATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKL
KAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLV
LDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGD
RNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFT
LTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVV
PRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQL
SVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGS
TASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSR
VEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQER
SHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPR
HYQLDQLPDYYDTPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_194449
ORF Size 3615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_194449.1, NP_919431.1
RefSeq Size 4798 bp
RefSeq ORF 5154 bp
Locus ID 23239
UniProt ID O60346
Cytogenetics 18q21.33
MW 184.5 kDa
Summary This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. provided by RefSeq, Dec 2011
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"NM_194449" in other vectors (6)
SKU Description Size Price
RC203930L1 Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), Myc-DDK-tagged 10 ug
$1,349.00
RC203930L2 Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mGFP tagged 10 ug
$1,349.00
RC203930L3 Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), Myc-DDK-tagged 10 ug
$1,349.00
RC203930L4 Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mGFP tagged 10 ug
$1,349.00
RG203930 PHLPP1 (tGFP-tagged) - Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1) 10 ug
$789.00 $1,249.00
SC108817 PHLPP1 (untagged)-Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1) 10 ug
$1,050.00

Plasmid Map for RC203930

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203930
Circular map for RC203930

file-alt Citations

PHLPP1 splice variants differentially regulate AKT and PKCα signaling in hippocampal neurons: characterization of PHLPP proteins in the adult hippocampus
Jackson, Verrier, Semple-Rowland et al
J Neurochem (2010) 115 (4), 941-55 DOI: 10.1111/j.1471-4159.2010.06984.x
Cited by 35
AI Summary: OriGene products, including anti-PHLPP1 antibody (Cat# TA324945) and PHLPP1 expression plasmids, were used in this study to detect and manipulate PHLPP1 protein levels, enabling the authors to characterize the distinct roles of PHLPP1 splice variants in regulating AKT and PKCα signaling in hippocampal neurons.
FigureFigure
Showing 1-1 of 1 papers.
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