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SF3A3 (NM_006802) Human Tagged ORF Clone

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SKU
RC203895
SF3A3 (Myc-DDK-tagged)-Human splicing factor 3a, subunit 3, 60kDa (SF3A3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $466.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SF3A3
Synonyms PRP9; PRPF9; SAP61; SF3a60
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203895 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACAATACTGGAGCAGCAGCGGCGCTATCATGAGGAGAAGGAACGGCTCATGGACGTCATGGCTA
AAGAGATGCTCACCAAGAAGTCCACGCTCCGGGACCAGATCAATTCTGATCACCGCACTCGGGCCATGCA
AGATAGGTATATGGAGGTCAGTGGGAACCTGAGGGATTTGTATGATGATAAGGATGGATTACGAAAGGAG
GAGCTCAATGCCATTTCAGGACCCAATGAGTTTGCTGAATTCTATAATAGACTCAAGCAAATAAAGGAAT
TCCACCGGAAGCACCCAAATGAGATCTGTGTGCCAATGTCAGTGGAATTTGAGGAACTCCTGAAGGCTCG
AGAGAATCCAAGTGAAGAGGCACAAAACTTGGTGGAGTTCACAGATGAGGAGGGATATGGTCGTTATCTC
GATCTCCATGACTGTTACCTCAAGTACATTAACCTGAAGGCATCTGAGAAGCTGGATTATATCACATACC
TGTCCATCTTTGACCAATTATTTGACATTCCTAAAGAAAGGAAGAATGCAGAGTATAAGAGATACCTAGA
GATGCTGCTTGAGTACCTTCAGGATTACACAGATAGAGTGAAGCCTCTCCAAGATCAGAATGAACTTTTT
GGGAAGATTCAGGCTGAGTTTGAGAAGAAATGGGAGAATGGGACCTTTCCTGGATGGCCGAAAGAGACAA
GCAGTGCCCTGACCCATGCTGGAGCCCATCTTGACCTCTCTGCATTCTCCTCCTGGGAGGAGTTGGCTTC
TCTGGGTTTGGACAGATTGAAATCTGCTCTCTTAGCTTTAGGCTTGAAATGTGGCGGGACCCTAGAAGAG
CGAGCCCAGAGACTATTCAGTACCAAAGGAAAGTCCCTGGAGTCACTTGATACCTCTTTGTTTGCCAAAA
ATCCCAAGTCAAAGGGCACCAAGCGAGACACTGAAAGGAACAAAGACATTGCTTTTCTAGAAGCCCAGAT
CTATGAATATGTAGAGATTCTCGGGGAACAGCGACATCTCACTCATGAAAATGTACAGCGCAAGCAAGCC
AGGACAGGAGAAGAGCGAGAAGAAGAGGAAGAAGAGCAGATCAGTGAGAGTGAGAGTGAAGATGAAGAGA
ACGAGATCATTTACAACCCCAAAAACCTGCCACTTGGCTGGGATGGCAAACCTATTCCCTACTGGCTGTA
TAAGCTTCATGGCCTAAATATCAACTACAACTGTGAGATTTGTGGAAACTACACCTACCGAGGGCCCAAA
GCCTTCCAGCGACACTTTGCTGAATGGCGTCATGCTCATGGCATGAGGTGTTTGGGCATCCCAAACACTG
CTCACTTTGCTAATGTGACACAGATTGAAGATGCTGTCTCCTTGTGGGCCAAACTGAAATTGCAGAAGGC
TTCAGAACGATGGCAGCCTGACACTGAGGAAGAATATGAAGACTCAAGTGGGAATGTTGTGAATAAGAAG
ACATACGAGGATCTGAAAAGACAAGGACTGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203895 protein sequence
Red=Cloning site Green=Tags(s)

METILEQQRRYHEEKERLMDVMAKEMLTKKSTLRDQINSDHRTRAMQDRYMEVSGNLRDLYDDKDGLRKE
ELNAISGPNEFAEFYNRLKQIKEFHRKHPNEICVPMSVEFEELLKARENPSEEAQNLVEFTDEEGYGRYL
DLHDCYLKYINLKASEKLDYITYLSIFDQLFDIPKERKNAEYKRYLEMLLEYLQDYTDRVKPLQDQNELF
GKIQAEFEKKWENGTFPGWPKETSSALTHAGAHLDLSAFSSWEELASLGLDRLKSALLALGLKCGGTLEE
RAQRLFSTKGKSLESLDTSLFAKNPKSKGTKRDTERNKDIAFLEAQIYEYVEILGEQRHLTHENVQRKQA
RTGEEREEEEEEQISESESEDEENEIIYNPKNLPLGWDGKPIPYWLYKLHGLNINYNCEICGNYTYRGPK
AFQRHFAEWRHAHGMRCLGIPNTAHFANVTQIEDAVSLWAKLKLQKASERWQPDTEEEYEDSSGNVVNKK
TYEDLKRQGLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006802
ORF Size 1503 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006802.4
RefSeq Size 2855 bp
RefSeq ORF 1506 bp
Locus ID 10946
UniProt ID Q12874
Cytogenetics 1p34.3
Protein Pathways Spliceosome
MW 58.8 kDa
Summary This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2016
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Documents
Resources
"NM_006802" in other vectors (7)
SKU Description Size Price
RC203895L1 Lenti ORF clone of Human splicing factor 3a, subunit 3, 60kDa (SF3A3), Myc-DDK-tagged 10 ug
$766.00
RC203895L2 Lenti ORF clone of Human splicing factor 3a, subunit 3, 60kDa (SF3A3), mGFP tagged 10 ug
$766.00
RC203895L3 Lenti ORF clone of Human splicing factor 3a, subunit 3, 60kDa (SF3A3), Myc-DDK-tagged 10 ug
$766.00
RC203895L4 Lenti ORF clone of Human splicing factor 3a, subunit 3, 60kDa (SF3A3), mGFP tagged 10 ug
$766.00
RG203895 SF3A3 (tGFP-tagged) - Human splicing factor 3a, subunit 3, 60kDa (SF3A3) 10 ug
$489.00 $665.00
SC115857 SF3A3 (untagged)-Human splicing factor 3a, subunit 3, 60kDa (SF3A3) 10 ug
$467.00
SC324580 SF3A3 (untagged)-Human splicing factor 3a, subunit 3, 60kDa (SF3A3) 10 ug
$467.00

Plasmid Map for RC203895

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203895
Circular map for RC203895

file-alt Citations

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