MAL2 (NM_052886) Human Tagged ORF Clone
SKU
RC203862
MAL2 (Myc-DDK-tagged)-Human mal, T-cell differentiation protein 2 (gene/pseudogene) (MAL2)
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MAL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_052886 |
| ORF Size | 528 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_052886.3 |
| RefSeq Size | 2831 bp |
| RefSeq ORF | 531 bp |
| Locus ID | 114569 |
| UniProt ID | Q969L2, A0A024R9E4 |
| Cytogenetics | 8q24.12 |
| Domains | MARVEL |
| Protein Families | Transmembrane |
| MW | 19.1 kDa |
| Summary | This gene encodes a multispan transmembrane protein belonging to the MAL proteolipid family. The protein is a component of lipid rafts and, in polarized cells, it primarily localizes to endosomal structures beneath the apical membrane. It is required for transcytosis, an intracellular transport pathway used to deliver membrane-bound proteins and exogenous cargos from the basolateral to the apical surface. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_052886" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203862L1 | Lenti ORF clone of Human mal, T-cell differentiation protein 2 (gene/pseudogene) (MAL2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC203862L2 | Lenti ORF clone of Human mal, T-cell differentiation protein 2 (gene/pseudogene) (MAL2), mGFP tagged | 10 ug |
$750.00
|
|
| RC203862L3 | Lenti ORF clone of Human mal, T-cell differentiation protein 2 (gene/pseudogene) (MAL2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC203862L4 | Lenti ORF clone of Human mal, T-cell differentiation protein 2 (gene/pseudogene) (MAL2), mGFP tagged | 10 ug |
$750.00
|
|
| RG203862 | MAL2 (tGFP-tagged) - Human mal, T-cell differentiation protein 2 (gene/pseudogene) (MAL2) | 10 ug |
$650.00
|
|
| SC120162 | MAL2 (untagged)-Human mal, T-cell differentiation protein 2 (gene/pseudogene) (MAL2) | 10 ug |
$300.00
|
|
Citations
MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells
Jeong, Shin, Li et al
Cell Rep (2021) 37 (13), 110160 DOI: 10.1016/j.celrep.2021.110160
Jeong, Shin, Li et al
Cell Rep (2021) 37 (13), 110160 DOI: 10.1016/j.celrep.2021.110160
Cited by 36
AI Summary: In this study, OriGene’s MAL2 shRNA (cat# TL313896) and MAL2 cDNA clone (cat# RC206307) were used to modulate MAL2 expression, enabling the authors to investigate MAL2’s role in HER2 signaling complex formation and membrane retention in breast cancer cells.
Snippet: The lipid raft-resident protein, MAL2, has been implicated as contributing to the pathogenesis of several malignancies, including breast cancer, but the underlying mechanism for its effects on tumorigenesis is unknown. Here, we show that MAL2-mediated lipid raft formation leads to HER2 plasma membrane retention and enhanced HER2 signaling in breast cancer cells. We demonstrate physical interactions between HER2 and MAL2 in lipid rafts using proximity ligation assays. …HER2/MAL2 protein interactions in lipid rafts are enhanced in trastuzumab-resistant breast cancer cells. Our findings suggest that MAL2 is crucial for lipid raft formation, HER2 signaling, and HER2 membrane stability in breast cancer cells, suggesting MAL2 as a potential therapeutic target.Copyright 2021 The Authors. …
Showing 1-1 of 1 papers.
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