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EFS (NM_032459) Human Tagged ORF Clone

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SKU
RC203800
EFS (Myc-DDK-tagged)-Human embryonal Fyn-associated substrate (EFS), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EFS
Synonyms CAS3; CASS3; EFS1; EFS2; HEFS; SIN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203800 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCATTGCCACGTCGGTGTATGTGGTGCCGCCCCCAGCTCGGCCCTGTCCAACCTCAGGACCTCCAG
CTGGACCTTGCCCACCCTCTCCTGACCTCATCTACAAAATCCCCAGAGCTAGTGGGACCCAGCTGGCTGC
TCCCAGAGATGCCTTGGAGGTCTACGATGTGCCCCCCACCGCCCTCCGGGTGCCCTCCAGTGGCCCCTAT
GACTGCCCTGCCTCCTTTTCCCACCCTCTGACCCGGGTTGCCCCGCAGCCCCCTGGAGAGGATGATGCTC
CCTATGATGTGCCTCTGACCCCAAAGCCACCTGCAGAGCTGGAACCAGATCTGGAGTGGGAAGGAGGCCG
GGAGCCGGGGCCCCCCATCTATGCTGCCCCCTCCAACCTGAAACGAGCGTCAGCCTTACTCAATTTGTAT
GAAGCACCCGAGGAACTGCTGGCAGACGGGGAGGGCGGGGGCACTGATGAGGGGATCTACGATGTGCCTC
TGCTGGGGCCAGAGGCTCCCCCTTCTCCAGAGCCCCCTGGAGCCTTGGCCTCCCATGACCAGGACACCCT
GGCCCAGCTTCTGGCCAGAAGCCCCCCACCCCCACACAGGCCCCGGCTCCCCTCAGCTGAGAGCCTGTCC
CGCCGCCCTCTGCCTGCCCTGCCTGTCCCTGAGGCCCCCAGCCCCTCCCCAGTGCCCTCTCCTGCCCCAG
GCCGGAAGGGCAGCATCCAGGACCGGCCTCTGCCCCCACCCCCACCCCGCCTGCCTGGTTATGGAGGCCC
CAAGGTCGAGGGGGATCCAGAGGGCAGGGAGATGGAGGATGACCCAGCAGGACACCACAATGAGTACGAG
GGCATTCCGATGGCCGAGGAGTATGACTATGTCCACCTGAAGGGCATGGACAAAGCTCAGGGATCTAGGC
CCCCGGATCAGGCCTGCACAGGGGATCCTGAACTGCCCGAGAGGGGGATGCCGGCGCCGCAGGAGGCCCT
GTCCCCAGGGGAGCCACTGGTTGTGTCCACCGGAGATCTGCAGCTCCTGTACTTCTATGCTGGGCAATGC
CAGAGCCACTACTCAGCCCTGCAGGCAGCCGTGGCAGCCCTGATGTCCAGTACCCAGGCTAATCAGCCCC
CGCGCCTTTTCGTGCCCCACAGCAAGAGGGTGGTGGTGGCTGCTCATCGCCTGGTGTTTGTTGGGGACAC
CCTGGGCCGGCTGGCAGCCTCTGCCCCTCTGAGAGCACAGGTCAGGGCTGCAGGTACAGCACTGGGCCAG
GCATTGCGGGCCACTGTGCTGGCTGTCAAGGGAGCTGCCCTGGGCTACCCATCCAGCCCTGCCATCCAAG
AGATGGTGCAGTGTGTAACAGAACTGGCAGGGCAGGCCCTGCAATTCACTACCCTGCTCACTAGCCTGGC
TCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203800 protein sequence
Red=Cloning site Green=Tags(s)

MAIATSVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPY
DCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLY
EAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLS
RRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYE
GIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEALSPGEPLVVSTGDLQLLYFYAGQC
QSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQ
ALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032459
ORF Size 1404 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032459.3
RefSeq Size 2851 bp
RefSeq ORF 1407 bp
Locus ID 10278
UniProt ID O43281
Cytogenetics 14q11.2
MW 49 kDa
Summary The protein encoded by this gene is a member of the CAS (CRK-associated substrate) family of adaptor proteins which typically serve as scaffolds for the assembly of larger signaling complexes. These complexes form at the cell surface where integrin binding leads to the subsequent phosphorylation of a CAS protein. Additional binding of SRC family kinases leads to CAS hyperphosphorylation and the creation of binding sites for CRK and other proteins that cause actin cytoskeleton reorganization. This gene plays a role in integrin-mediated cell attachment, spreading, and migration and also plays a role in both normal and malignant cellular transformation. This broadly expressed gene has been shown to play a role in neurite outgrowth and its expression in the thymus and lymphocytes is important for T cell maturation and the development of immunological self-tolerance. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2020
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Documents
Resources
"NM_032459" in other vectors (5)
SKU Description Size Price
RC203800L3 Lenti ORF clone of Human embryonal Fyn-associated substrate (EFS), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC203800L4 Lenti ORF clone of Human embryonal Fyn-associated substrate (EFS), transcript variant 2, mGFP tagged 10 ug
$757.00
RG203800 EFS (tGFP-tagged) - Human embryonal Fyn-associated substrate (EFS), transcript variant 2 10 ug
$489.00 $657.00
SC110138 EFS (untagged)-Human embryonal Fyn-associated substrate (EFS), transcript variant 2 10 ug
$457.00
SC323937 EFS (untagged)-Human embryonal Fyn-associated substrate (EFS), transcript variant 2 10 ug
$457.00

Plasmid Map for RC203800

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203800
Circular map for RC203800

file-alt Citations

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