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GLOD4 (NM_016080) Human Tagged ORF Clone

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SKU
RC203760
GLOD4 (Myc-DDK-tagged)-Human glyoxalase domain containing 4 (GLOD4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GLOD4
Synonyms C17orf25; CGI-150; HC71
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203760 representing NM_016080
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTCGCAGAGCTCTGCACTTCGTATTCAAAGTGGGAAACCGCTTCCAGACGGCGCGTTTCTATC
GGGACGTCCTGGGGATGAAGGTTCTGCGGCATGAGGAATTTGAAGAAGGCTGCAAAGCTGCCTGTAATGG
GCCTTATGATGGGAAATGGAGTAAAACAATGGTGGGATTTGGGCCTGAGGATGATCATTTTGTCGCAGAA
CTGACTTACAATTATGGCGTCGGAGACTACAAGCTTGGCAATGACTTTATGGGAATCACGCTCGCTTCTA
GCCAGGCTGTCAGCAACGCCAGGAAGCTGGAGTGGCCACTGACGGAAGTTGCAGAAGGTGTTTTTGAAAC
CGAGGCCCCGGGAGGATATAAGTTCTATTTGCAGAATCGCAGTCTGCCTCAGTCAGATCCTGTATTAAAA
GTAACTCTAGCAGTGTCTGATCTTCAAAAGTCCTTGAACTACTGGTGTAATCTACTGGGAATGAAAATTT
ATGAAAAAGATGAAGAAAAGCAAAGGGCTTTGCTGGGCTATGCTGATAACCAGTGTAAGCTGGAGCTACA
GGGCGTCAAGGGTGGGGTGGACCATGCAGCAGCTTTTGGAAGAATTGCCTTCTCTTGCCCCCAGAAAGAG
TTGCCAGACTTAGAAGACTTGATGAAAAGGGAGAACCAGAAGATTCTGACTCCCCTGGTGAGCCTGGACA
CCCCAGGGAAAGCAACAGTACAGGTGGTCATTCTGGCCGACCCTGACGGACATGAAATTTGCTTTGTCGG
GGATGAAGCATTTCGAGAACTTTCTAAGATGGATCCAGAGGGAAGCAAATTGTTGGATGATGCAATGGCA
GCAGATAAAAGTGACGAGTGGTTTGCCAAACACAATAAACCCAAAGCTTCAGGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203760 representing NM_016080
Red=Cloning site Green=Tags(s)

MAARRALHFVFKVGNRFQTARFYRDVLGMKVLRHEEFEEGCKAACNGPYDGKWSKTMVGFGPEDDHFVAE
LTYNYGVGDYKLGNDFMGITLASSQAVSNARKLEWPLTEVAEGVFETEAPGGYKFYLQNRSLPQSDPVLK
VTLAVSDLQKSLNYWCNLLGMKIYEKDEEKQRALLGYADNQCKLELQGVKGGVDHAAAFGRIAFSCPQKE
LPDLEDLMKRENQKILTPLVSLDTPGKATVQVVILADPDGHEICFVGDEAFRELSKMDPEGSKLLDDAMA
ADKSDEWFAKHNKPKASG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016080
ORF Size 894 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016080.3
RefSeq Size 1797 bp
RefSeq ORF 897 bp
Locus ID 51031
UniProt ID Q9HC38
Cytogenetics 17p13.3
MW 33.1 kDa
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Documents
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"NM_016080" in other vectors (5)
SKU Description Size Price
RC203760L3 Lenti ORF clone of Human glyoxalase domain containing 4 (GLOD4), Myc-DDK-tagged 10 ug
$600.00
RC203760L4 Lenti ORF clone of Human glyoxalase domain containing 4 (GLOD4), mGFP tagged 10 ug
$600.00
RG203760 GLOD4 (tGFP-tagged) - Human glyoxalase domain containing 4 (GLOD4) 10 ug
$500.00
SC114439 GLOD4 (untagged)-Human glyoxalase domain containing 4 (GLOD4) 10 ug
$300.00
SC324130 GLOD4 (untagged)-Human glyoxalase domain containing 4 (GLOD4) 10 ug
$300.00

Plasmid Map for RC203760

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203760
Circular map for RC203760

file-alt Citations

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