DECR2 (NM_020664) Human Tagged ORF Clone

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SKU
RC203715
DECR2 (Myc-DDK-tagged)-Human 2,4-dienoyl CoA reductase 2, peroxisomal (DECR2)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DECR2
Synonyms PDCR; SDR17C1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203715 representing NM_020664
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGCCGCCGCCCGACGTGGAGGGGGACGACTGTCTCCCCGCGTACCGCCACCTCTTCTGCCCGG
ACCTGCTGCGGGACAAAGTGGCCTTCATCACAGGAGGCGGCTCTGGGATTGGGTTCCGGATTGCTGAGAT
TTTCATGCGGCACGGCTGCCATACGGTGATTGCCAGTAGGAGCCTGCCGCGAGTGCTGACGGCCGCCAGG
AAGCTGGCTGGGGCCACCGGCCGGCGCTGCCTCCCTCTCTCTATGGACGTCCGAGCGCCCCCAGCTGTCA
TGGCCGCCGTGGACCAGGCTCTGAAGGAGTTTGGCAGAATCGACATTCTCATTAACTGTGCGGCCGGGAA
CTTCCTGTGCCCCGCTGGCGCCTTGTCCTTCAACGCCTTCAAGACCGTGATGGACATCGATACCAGCGGC
ACCTTCAATGTGTCTCGTGTGCTCTATGAGAAGTTCTTCCGGGACCACGGAGGGGTGATCGTGAACATCA
CTGCCACCCTGGGGAACCGGGGGCAGGCGCTCCAGGTGCATGCAGGCTCCGCCAAGGCCGCTGTGGACGC
GATGACGCGGCACTTGGCTGTGGAGTGGGGTCCCCAAAACATCCGCGTCAACAGCCTCGCCCCTGGCCCC
ATCAGTGGCACAGAGGGGCTCCGGCGACTGGGTGGCCCTCAAGCCAGCCTGAGCACCAAGGTCACTGCCA
GCCCGCTGCAGAGGCTGGGGAACAAGACCGAGATCGCCCACAGCGTGCTCTACCTGGCCAGCCCTCTGGC
TTCCTACGTGACGGGGGCCGTGCTGGTGGCCGATGGCGGGGCATGGTTGACGTTCCCAAACGGTGTCAAA
GGGCTGCCGGATTTCGCATCCTTCTCTGCTAAGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203715 representing NM_020664
Red=Cloning site Green=Tags(s)

MAQPPPDVEGDDCLPAYRHLFCPDLLRDKVAFITGGGSGIGFRIAEIFMRHGCHTVIASRSLPRVLTAAR
KLAGATGRRCLPLSMDVRAPPAVMAAVDQALKEFGRIDILINCAAGNFLCPAGALSFNAFKTVMDIDTSG
TFNVSRVLYEKFFRDHGGVIVNITATLGNRGQALQVHAGSAKAAVDAMTRHLAVEWGPQNIRVNSLAPGP
ISGTEGLRRLGGPQASLSTKVTASPLQRLGNKTEIAHSVLYLASPLASYVTGAVLVADGGAWLTFPNGVK
GLPDFASFSAKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020664
ORF Size 876 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020664.2
RefSeq Size 1599 bp
RefSeq ORF 879 bp
Locus ID 26063
UniProt ID Q9NUI1
Cytogenetics 16p13.3
Domains adh_short
Protein Families Druggable Genome
MW 30.6 kDa
Summary Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid.UniProtKB/Swiss-Prot Function
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Documents
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"NM_020664" in other vectors (5)
SKU Description Size Price
RC203715L3 Lenti ORF clone of Human 2,4-dienoyl CoA reductase 2, peroxisomal (DECR2), Myc-DDK-tagged 10 ug
$750.00
RC203715L4 Lenti ORF clone of Human 2,4-dienoyl CoA reductase 2, peroxisomal (DECR2), mGFP tagged 10 ug
$750.00
RG203715 DECR2 (tGFP-tagged) - Human 2,4-dienoyl CoA reductase 2, peroxisomal (DECR2) 10 ug
$650.00
SC113040 DECR2 (untagged)-Human 2,4-dienoyl CoA reductase 2, peroxisomal (DECR2) 10 ug
$450.00
SC322397 DECR2 (untagged)-Human 2,4-dienoyl CoA reductase 2, peroxisomal (DECR2) 10 ug
$450.00

Plasmid Map for RC203715

Download sequence for

Cloning scheme for RC203715

Circular map for RC203715

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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