PANK3 (NM_024594) Human Tagged ORF Clone

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SKU
RC203696
PANK3 (Myc-DDK-tagged)-Human pantothenate kinase 3 (PANK3)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PANK3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203696 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATCAAAGATGCCAAGAAACCCTCTTTCCCATGGTTTGGCATGGACATTGGGGGAACTCTAGTAA
AGCTCTCGTACTTTGAACCTATTGATATCACAGCAGAGGAAGAGCAAGAAGAAGTTGAGAGTTTAAAAAG
TATTCGGAAATATTTGACTTCTAACGTGGCATATGGATCCACCGGCATTCGGGATGTACACCTTGAACTG
AAAGATTTAACACTTTTTGGCCGAAGAGGGAACTTGCACTTTATCAGGTTTCCAACCCAGGACCTGCCTA
CTTTTATCCAAATGGGAAGAGATAAAAACTTCTCAACATTGCAGACGGTGCTATGTGCTACAGGAGGTGG
TGCTTACAAGTTTGAAAAAGATTTTCGCACAATTGGAAACCTCCACCTGCACAAACTGGATGAACTTGAC
TGCCTTGTAAAGGGCTTGCTGTATATAGACTCTGTCAGTTTCAATGGACAAGCCGAGTGCTATTATTTTG
CTAATGCCTCAGAACCTGAGCGATGCCAAAAGATGCCTTTTAACCTGGATGATCCCTATCCACTGCTTGT
AGTGAACATTGGCTCAGGAGTCAGTATTTTAGCAGTCCATTCCAAAGACAACTATAAACGAGTGACTGGG
ACAAGCCTTGGAGGGGGTACCTTTCTGGGTTTATGCAGTTTATTGACTGGCTGTGAAAGTTTTGAAGAGG
CTCTTGAAATGGCATCCAAAGGTGATAGCACACAAGCTGACAAGCTGGTCCGTGATATTTATGGAGGAGA
TTATGAAAGATTTGGTTTGCCAGGTTGGGCTGTAGCATCTAGTTTTGGGAATATGATTTATAAGGAGAAG
CGAGAATCTGTTAGTAAAGAAGATCTGGCAAGAGCTACTTTAGTTACTATCACCAATAACATTGGTTCTG
TGGCACGAATGTGTGCTGTTAATGAGAAAATAAACAGAGTTGTCTTTGTTGGAAACTTTTTACGTGTCAA
TACCCTCTCAATGAAACTTTTGGCATATGCACTGGATTACTGGTCAAAAGGTCAACTAAAAGCATTGTTT
CTAGAACATGAGGGTTACTTTGGAGCAGTTGGTGCACTTCTTGGGCTGCCAAATTTCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203696 protein sequence
Red=Cloning site Green=Tags(s)

MKIKDAKKPSFPWFGMDIGGTLVKLSYFEPIDITAEEEQEEVESLKSIRKYLTSNVAYGSTGIRDVHLEL
KDLTLFGRRGNLHFIRFPTQDLPTFIQMGRDKNFSTLQTVLCATGGGAYKFEKDFRTIGNLHLHKLDELD
CLVKGLLYIDSVSFNGQAECYYFANASEPERCQKMPFNLDDPYPLLVVNIGSGVSILAVHSKDNYKRVTG
TSLGGGTFLGLCSLLTGCESFEEALEMASKGDSTQADKLVRDIYGGDYERFGLPGWAVASSFGNMIYKEK
RESVSKEDLARATLVTITNNIGSVARMCAVNEKINRVVFVGNFLRVNTLSMKLLAYALDYWSKGQLKALF
LEHEGYFGAVGALLGLPNFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024594
ORF Size 1110 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024594.4
RefSeq Size 3404 bp
RefSeq ORF 1113 bp
Locus ID 79646
UniProt ID Q9H999
Cytogenetics 5q34
Domains Fumble
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Pantothenate and CoA biosynthesis
MW 41.1 kDa
Summary This gene encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is expressed most abundantly in the liver. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_024594" in other vectors (4)
SKU Description Size Price
RC203696L3 Lenti ORF clone of Human pantothenate kinase 3 (PANK3), Myc-DDK-tagged 10 ug
$757.00
RC203696L4 Lenti ORF clone of Human pantothenate kinase 3 (PANK3), mGFP tagged 10 ug
$757.00
RG203696 PANK3 (tGFP-tagged) - Human pantothenate kinase 3 (PANK3) 10 ug
$489.00 $657.00
SC322472 PANK3 (untagged)-Human pantothenate kinase 3 (PANK3) 10 ug
$457.00

Plasmid Map for RC203696

Download sequence for

Cloning scheme for RC203696

Circular map for RC203696

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