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SLC25A22 (NM_024698) Human Tagged ORF Clone

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SKU
RC203650
SLC25A22 (Myc-DDK-tagged)-Human solute carrier family 25 (mitochondrial carrier: glutamate), member 22 (SLC25A22), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC25A22
Synonyms DEE3; EIEE3; GC-1; GC1; NET44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203650 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGATAAGCAGATCAGCCTGCCAGCCAAGCTCATCAATGGCGGCATCGCCGGGCTGATCGGTGTCA
CCTGCGTGTTTCCCATCGACCTGGCCAAGACCAGGCTGCAGAACCAGCAGAACGGCCAGCGCGTGTACAC
GAGCATGTCCGACTGCCTCATCAAGACCGTCCGCTCCGAGGGCTACTTCGGCATGTACCGGGGAGCTGCT
GTGAACTTGACCCTCGTCACCCCCGAGAAGGCCATCAAGCTGGCAGCCAACGACTTCTTCCGACATCAGC
TCTCTAAGGACGGGCAGAAGCTGACCCTGCTTAAAGAGATGCTGGCGGGCTGTGGGGCTGGCACCTGCCA
GGTGATCGTGACCACGCCCATGGAGATGCTGAAGATCCAGCTGCAGGATGCAGGGCGCATTGCCGCCCAG
AGGAAGATCCTGGCTGCCCAGGGCCAGCTCTCGGCCCAGGGGGGTGCCCAGCCCTCAGTGGAGGCTCCAG
CTGCCCCTCGGCCCACGGCCACCCAGCTGACCCGCGACCTGCTGCGGAGCCGTGGCATTGCCGGTCTCTA
CAAGGGACTCGGGGCCACGCTGCTCAGGGATGTCCCCTTCTCTGTGGTGTACTTCCCGCTCTTTGCCAAC
CTGAACCAGCTGGGCCGCCCGGCGTCCGAGGAGAAGTCGCCTTTCTACGTGTCCTTCCTGGCCGGCTGTG
TGGCTGGGAGTGCCGCCGCTGTGGCCGTCAACCCCTGTGATGTGGTGAAGACGCGGCTCCAGTCACTTCA
GCGAGGCGTCAACGAGGACACCTACTCTGGGATCCTGGACTGTGCCAGGAAGATCCTGCGGCACGAGGGC
CCCTCGGCCTTCCTGAAGGGCGCCTACTGCCGCGCGCTGGTCATCGCGCCCCTTTTCGGCATCGCACAGG
TGGTCTACTTCCTGGGCATCGCGGAGTCCCTGCTGGGGCTGCTGCAGGACCCCCAGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203650 protein sequence
Red=Cloning site Green=Tags(s)

MADKQISLPAKLINGGIAGLIGVTCVFPIDLAKTRLQNQQNGQRVYTSMSDCLIKTVRSEGYFGMYRGAA
VNLTLVTPEKAIKLAANDFFRHQLSKDGQKLTLLKEMLAGCGAGTCQVIVTTPMEMLKIQLQDAGRIAAQ
RKILAAQGQLSAQGGAQPSVEAPAAPRPTATQLTRDLLRSRGIAGLYKGLGATLLRDVPFSVVYFPLFAN
LNQLGRPASEEKSPFYVSFLAGCVAGSAAAVAVNPCDVVKTRLQSLQRGVNEDTYSGILDCARKILRHEG
PSAFLKGAYCRALVIAPLFGIAQVVYFLGIAESLLGLLQDPQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024698
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024698.6
RefSeq Size 2822 bp
RefSeq ORF 972 bp
Locus ID 79751
UniProt ID Q9H936
Cytogenetics 11p15.5
Domains mito_carr
Protein Families Transmembrane
MW 34.5 kDa
Summary This gene encodes a mitochondrial glutamate carrier. Mutations in this gene are associated with early infantile epileptic encephalopathy. Multiple alternatively spliced variants, encoding the same protein, have been identified.provided by RefSeq, Jul 2010
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"NM_024698" in other vectors (4)
SKU Description Size Price
RC203650L3 Lenti ORF clone of Human solute carrier family 25 (mitochondrial carrier: glutamate), member 22 (SLC25A22), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC203650L4 Lenti ORF clone of Human solute carrier family 25 (mitochondrial carrier: glutamate), member 22 (SLC25A22), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged 10 ug
$600.00
RG203650 SLC25A22 (tGFP-tagged) - Human solute carrier family 25 (mitochondrial carrier: glutamate), member 22 (SLC25A22), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$489.00 $500.00
SC319589 SLC25A22 (untagged)-Human solute carrier family 25 (mitochondrial carrier: glutamate), member 22 (SLC25A22), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$300.00

Plasmid Map for RC203650

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203650
Circular map for RC203650

file-alt Citations

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