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MCM7 (NM_182776) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC203630
MCM7 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $659.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MCM7
Synonyms CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203630 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACTGAAGGACTACGCGCTAGAGAAGGAAAAGGTTAAGAAGTTCTTACAAGAGTTCTACCAGGATG
ATGAACTCGGGAAGAAGCAGTTCAAGTATGGGAACCAGTTGGTTCGGCTGGCTCATCGGGAACAGGTGGC
TCTGTATGTGGACCTGGACGACGTAGCCGAGGATGACCCCGAGTTGGTGGACTCAATTTGTGAGAATGCC
AGGCGCTACGCGAAGCTCTTTGCTGATGCCGTACAAGAGCTGCTGCCTCAGTACAAGGAGAGGGAAGTGG
TAAATAAAGATGTCCTGGACGTTTACATTGAGCATCGGCTAATGATGGAGCAGCGGAGTCGGGACCCTGG
GATGGTCCGAAGCCCCCAGAACCAGTACCCTGCTGAACTCATGCGCAGATTTGAGCTGTATTTTCAAGGC
CCTAGCAGCAACAAGCCTCGTGTGATCCGGGAAGTGCGGGCTGACTCTGTGGGGAAGTTGGTAACTGTGC
GTGGAATCGTCACTCGTGTCTCTGAAGTCAAACCCAAGATGGTGGTGGCCACTTACACTTGTGACCAGTG
TGGGGCAGAGACCTACCAGCCGATCCAGTCTCCCACTTTCATGCCTCTGATCATGTGCCCAAGCCAGGAG
TGCCAAACCAACCGCTCAGGAGGGCGGCTGTATCTGCAGACACGGGGCTCCAGATTCATCAAATTCCAGG
AGATGAAGATGCAAGAACATAGTGATCAGGTGCCTGTGGGAAATATCCCTCGTAGTATCACGGTGCTGGT
AGAAGGAGAGAACACAAGGATTGCCCAGCCTGGAGACCACGTCAGCGTCACTGGTATTTTCTTGCCAATC
CTGCGCACTGGGTTCCGACAGGTGGTACAGGGTTTACTCTCAGAAACCTACCTGGAAGCCCATCGGATTG
TGAAGATGAACAAGAGTGAGGATGATGAGTCTGGGGCTGGAGAGCTCACCAGGGAGGAGCTGAGGCAAAT
TGCAGAGGAGGATTTCTACGAAAAGCTGGCAGCTTCAATCGCCCCAGAAATATACGGGCATGAAGATGTG
AAGAAGGCACTGCTGCTCCTGCTAGTCGGGGGTGTGGACCAGTCTCCTCGAGGCATGAAAATCCGGGGCA
ACATCAACATCTGTCTGATGGGGGATCCTGGTGTGGCCAAGTCTCAGCTCCTGTCATACATTGATCGACT
GGCGCCTCGCAGCCAGTACACAACAGGCCGGGGCTCCTCAGGAGTGGGGCTTACGGCAGCTGTGCTGAGA
GACTCCGTGAGTGGAGAACTGACCTTAGAGGGTGGGGCCCTGGTGCTGGCTGACCAGGGTGTGTGCTGCA
TTGATGAGTTCGACAAGATGGCTGAGGCCGACCGCACAGCCATCCACGAGGTCATGGAGCAGCAGACCAT
CTCCATTGCCAAGGCCGGCATTCTCACCACACTCAATGCCCGCTGCTCCATCCTGGCTGCCGCCAACCCT
GCCTACGGGCGCTACAACCCTCGCCGCAGCCTGGAGCAGAACATACAGCTACCTGCTGCACTGCTCTCCC
GGTTTGACCTCCTCTGGCTGATTCAGGACCGGCCCGACCGAGACAATGACCTACGGTTGGCCCAGCACAT
TACCTATGTGCACCAGCACAGCCGGCAGCCCCCCTCCCAGTTTGAACCTCTGGACATGAAGCTCATGAGG
CGTTACATAGCCATGTGCCGCGAGAAGCAGCCCATGGTGCCAGAGTCTCTGGCTGACTACATCACAGCAG
CATACGTGGAGATGAGGCGAGAGGCTTGGGCTAGTAAGGATGCCACCTATACTTCTGCCCGGACCCTGCT
GGCTATCCTGCGCCTTTCCACTGCTCTGGCACGTCTGAGAATGGTGGATGTGGTGGAGAAAGAAGATGTG
AATGAAGCCATCAGGCTAATGGAGATGTCAAAGGACTCTCTTCTAGGAGACAAGGGGCAGACAGCTAGGA
CTCAGAGACCAGCAGATGTGATATTTGCCACCGTCCGTGAACTGGTCTCAGGGGGCCGAAGTGTCCGGTT
CTCTGAGGCAGAGCAGCGCTGTGTATCTCGTGGCTTCACACCCGCCCAGTTCCAGGCGGCTCTGGATGAA
TATGAGGAGCTCAATGTCTGGCAGGTCAATGCTTCCCGGACACGGATCACTTTTGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203630 protein sequence
Red=Cloning site Green=Tags(s)

MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENA
RRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQG
PSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQE
CQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPI
LRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDV
KKALLLLLVGGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR
DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANP
AYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPSQFEPLDMKLMR
RYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAILRLSTALARLRMVDVVEKEDV
NEAIRLMEMSKDSLLGDKGQTARTQRPADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDE
YEELNVWQVNASRTRITFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182776
ORF Size 2157 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 2996 bp
RefSeq ORF 1632 bp
Locus ID 4176
UniProt ID P33993
Cytogenetics 7q22.1
Protein Families Transcription Factors
Protein Pathways Cell cycle, DNA replication
MW 81.3 kDa
Summary The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_182776" in other vectors (4)
SKU Description Size Price
RC203630L3 Lenti ORF clone of Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2, Myc-DDK-tagged 10 ug
$959.00
RC203630L4 Lenti ORF clone of Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2, mGFP tagged 10 ug
$959.00
RG203630 MCM7 (tGFP-tagged) - Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2 10 ug
$859.00
SC109412 MCM7 (untagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2 10 ug
$506.00

Plasmid Map for RC203630

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203630
Circular map for RC203630

file-alt Citations

Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion
Lau, Chan, Pang et al
Oncogene (2010) 29 (40), 5475-89 DOI: 10.1038/onc.2010.287
Cited by 89
AI Summary: OriGene products, specifically the MCM2, MCM3, and MCM7 cDNA clones and siRNAs, were used in this study to manipulate the expression of these genes in medulloblastoma cell lines, enabling the authors to investigate their roles in tumor cell growth, migration, and invasion.
Showing 1-1 of 1 papers.
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