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MCM7 (NM_182776) Human Untagged Clone

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SKU
SC109412
MCM7 (untagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2
  $506.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MCM7
Synonyms CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_182776, the custom clone sequence may differ by one or more nucleotides


ATGGTGGTGGCCACTTACACTTGTGACCAGTGTGGGGCAGAGACCTACCAGCCGATCCAGTCTCCCACTT
TCATGCCTCTGATCATGTGCCCAAGCCAGGAGTGCCAAACCAACCGCTCAGGAGGGCGGCTGTATCTGCA
GACACGGGGCTCCAGATTCATCAAATTCCAGGAGATGAAGATGCAAGAACATAGTGATCAGGTGCCTGTG
GGAAATATCCCTCGTAGTATCACGGTGCTGGTAGAAGGAGAGAACACAAGGATTGCCCAGCCTGGAGACC
ACGTCAGCGTCACTGGTATTTTCTTGCCAATCCTGCGCACTGGGTTCCGACAGGTGGTACAGGGTTTACT
CTCAGAAACCTACCTGGAAGCCCATCGGATTGTGAAGATGAACAAGAGTGAGGATGATGAGTCTGGGGCT
GGAGAGCTCACCAGGGAGGAGCTGAGGCAAATTGCAGAGGAGGATTTCTACGAAAAGCTGGCAGCTTCAA
TCGCCCCAGAAATATACGGGCATGAAGATGTGAAGAAGGCACTGCTGCTCCTGCTAGTCGGGGGTGTGGA
CCAGTCTCCTCGAGGCATGAAAATCCGGGGCAACATCAACATCTGTCTGATGGGGGATCCTGGTGTGGCC
AAGTCTCAGCTCCTGTCATACATTGATCGACTGGCGCCTCGCAGCCAGTACACAACAGGCCGGGGCTCCT
CAGGAGTGGGGCTTACGGCAGCTGTGCTGAGAGACTCCGTGAGTGGAGAACTGACCTTAGAGGGTGGGGC
CCTGGTGCTGGCTGACCAGGGTGTGTGCTGCATTGATGAGTTCGACAAGATGGCTGAGGCCGACCGCACA
GCCATCCACGAGGTCATGGAGCAGCAGACCATCTCCATTGCCAAGGCCGGCATTCTCACCACACTCAATG
CCCGCTGCTCCATCCTGGCTGCCGCCAACCCTGCCTACGGGCGCTACAACCCTCGCCGCAGCCTGGAGCA
GAACATACAGCTACCTGCTGCACTGCTCTCCCGGTTTGACCTCCTCTGGCTGATTCAGGACCGGCCCGAC
CGAGACAATGACCTACGGTTGGCCCAGCACATCACCTATGTGCACCAGCACAGCCGGCAGCCCCCCTCCC
AGTTTGAACCTCTGGACATGAAGCTCATGAGGCGTTACATAGCCATGTGCCGCGAGAAGCAGCCCATGGT
GCCAGAGTCTCTGGCTGACTACATCACAGCAGCATACGTGGAGATGAGGCGAGAGGCTTGGGCTAGTAAG
GATGCCACCTATACTTCTGCCCGGACCCTGCTGGCTATCCTGCGCCTTTCCACTGCTCTGGCACGTCTGA
GAATGGTGGATGTGGTGGAGAAAGAAGATGTGAATGAAGCCATCAGGCTAATGGAGATGTCAAAGGACTC
TCTTCTAGGAGACAAGGGGCAGACAGCTAGGACTCAGAGACCAGCAGATGTGATATTTGCCACCGTCCGT
GAACTGGTCTCAGGGGGCCGAAGTGTCCGGTTCTCTGAGGCAGAGCAGCGCTGTGTATCTCGTGGCTTCA
CACCCGCCCAGTTCCAGGCGGCTCTGGATGAATATGAGGAGCTCAATGTCTGGCAGGTCAATGCTTCCCG
GACACGGATCACTTTTGTCTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_182776 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGAGGTCCCGGTGAAAT
CAGGGGCTAAGGGGACCCAAAGAAGGCGGGGGATCATAGGGGTGGAAAGAAAGCTGAGAA
CCTTGAGACCGGAGTGTGAGGGGCCAACGGGGAAGGGCGCTAGAATTTTAAACTAAAGTA
GGGACCGGAATTCCCCTGGGGAGATGTTGGATGGCCCTGTGCACTGCCACGGGCTCTTTA
TTCTTCGCTGGTTAGAAACAGACTTGTGAAAAAGAGTTATGCCCACTTTGGGGAGACTTC
GAAAAGGTTAAGAAGTTCTTACAAGAGTTCTACCAGGATGATGAACTCGGGAAGAAGCAG
TTCAAGTATGGGAACCAGTTGGTTCGGCTGGCTCATCGGGAACAGGTGGCTCTGTATGTG
GACCTGGACGACGTAGCCGAGGATGACCCCGAGTTGGTGGACTCAATTTGTGAGAATGCC
AGGCGCTACGCGAAGCTCTTTGCTGATGCCGTACAAGAGCTGCTGCCTCAGTACAAGGAG
AGGGAAGTGGTAAATAAAGATGTCCTGGACGTTTACATTGAGCATCGGCTAATGATGGAG
CAGCGGAGTCGGGACCCTGGGATGGTCCGAAGCCCCCAGAACCAGTACCCTGCTGAACTC
ATGCGCAGATTGTGAGTGGTCTCTGTCGGGAAAGATGTANGGATTGGTTCTCCAGGATCT
TGGTTGTGACTGTTNTCTCCCCTTAGTGAGCTGTATTTTCAGGCCCTAGCAGCAGCAAGC
CTCGTGTGATCCGGGAAGTGCGGGCTGACTCTGGGGGGAAGTGGTAACTGTGCGTGAATC
GTACTCGTGCTCTGAGTCAAACCCAGAGGTGGTGGCACTACACTGTGACACGGTGGGAGA
GACTACAGNCGATCAGCTNCCATTTCATGCCTGT
Restriction Sites NotI-NotI |
ACCN NM_182776
Insert Size 2800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182776.1, NP_877577.1
RefSeq Size 2900 bp
RefSeq ORF 1632 bp
Locus ID 4176
UniProt ID P33993
Cytogenetics 7q22.1
Protein Families Transcription Factors
Protein Pathways Cell cycle, DNA replication
Summary The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) differs at the 5' end, contains an additional internal segment, and initiates translation from an in-frame downstream AUG compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform.
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Documents
Resources
"NM_182776" in other vectors (4)
SKU Description Size Price
RC203630 MCM7 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2 10 ug
$589.00 $659.00
RC203630L3 Lenti ORF clone of Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2, Myc-DDK-tagged 10 ug
$959.00
RC203630L4 Lenti ORF clone of Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2, mGFP tagged 10 ug
$959.00
RG203630 MCM7 (tGFP-tagged) - Human minichromosome maintenance complex component 7 (MCM7), transcript variant 2 10 ug
$859.00

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