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RCC1 (NM_001048199) Human Tagged ORF Clone

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SKU
RC203556
RCC1 (Myc-DDK-tagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RCC1
Synonyms CHC1; RCC1-I; SNHG3-RCC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203556 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCACCCAAGCGCATAGCTAAAAGAAGGTCCCCCCCAGCAGATGCCATCCCCAAAAGCAAGAAGGTGA
AGGTCTCACACAGGTCCCACAGCACAGAACCCGGCTTGGTGCTGACACTAGGCCAGGGCGACGTGGGCCA
GCTGGGGCTGGGTGAGAATGTGATGGAGAGGAAGAAGCCGGCCCTGGTATCCATTCCGGAGGATGTTGTG
CAGGCTGAGGCTGGGGGCATGCACACCGTGTGTCTAAGCAAAAGTGGCCAGGTCTATTCCTTCGGCTGCA
ATGATGAGGGTGCCCTGGGAAGGGACACATCAGTGGAGGGCTCGGAGATGGTCCCTGGGAAAGTGGAGCT
GCAAGAGAAGGTGGTACAGGTGTCAGCAGGAGACAGTCACACAGCAGCCCTCACCGATGATGGCCGTGTC
TTCCTCTGGGGCTCCTTCCGGGACAATAACGGTGTGATTGGACTGTTGGAGCCCATGAAGAAGAGCATGG
TGCCTGTGCAGGTGCAGCTGGATGTGCCTGTGGTAAAGGTGGCCTCAGGAAACGACCACTTGGTGATGCT
GACAGCTGATGGTGACCTCTACACCTTGGGCTGCGGGGAACAGGGCCAGCTAGGCCGTGTGCCTGAGTTA
TTTGCCAACCGTGGTGGCCGGCAAGGCCTCGAACGACTCCTGGTCCCCAAGTGTGTGATGCTGAAATCCA
GGGGAAGCCGGGGCCACGTGAGATTCCAGGATGCCTTTTGTGGTGCCTATTTCACCTTTGCCATCTCCCA
TGAGGGCCACGTGTACGGCTTCGGCCTCTCCAACTACCATCAGCTTGGAACTCCGGGCACAGAATCTTGC
TTCATACCCCAGAACCTAACATCCTTCAAGAATTCCACCAAGTCCTGGGTGGGCTTCTCTGGTGGCCAGC
ACCATACAGTCTGCATGGATTCGGAAGGAAAAGCATACAGCCTGGGCCGGGCTGAGTATGGGCGGCTGGG
CCTTGGAGAGGGTGCTGAGGAGAAGAGCATACCCACCCTCATCTCCAGGCTGCCTGCTGTCTCCTCGGTG
GCTTGTGGGGCCTCTGTGGGGTATGCTGTGACCAAGGATGGTCGTGTTTTCGCCTGGGGCATGGGCACCA
ACTACCAGCTGGGCACAGGGCAGGATGAGGACGCCTGGAGCCCTGTGGAGATGATGGGCAAACAGCTGGA
GAACCGTGTGGTCTTATCTGTGTCCAGCGGGGGCCAGCATACAGTCTTATTAGTCAAGGACAAAGAACAG
AGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203556 protein sequence
Red=Cloning site Green=Tags(s)

MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVV
QAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRV
FLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPEL
FANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESC
FIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSV
ACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQ
S

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001048199
ORF Size 1263 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001048199.3
RefSeq Size 2715 bp
RefSeq ORF 1266 bp
Locus ID 1104
UniProt ID P18754
Cytogenetics 1p35.3
MW 45 kDa
Summary Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001048199" in other vectors (4)
SKU Description Size Price
RC203556L3 Lenti ORF clone of Human regulator of chromosome condensation 1 (RCC1), transcript variant 4, Myc-DDK-tagged 10 ug
$757.00
RC203556L4 Lenti ORF clone of Human regulator of chromosome condensation 1 (RCC1), transcript variant 4, mGFP tagged 10 ug
$757.00
RG203556 RCC1 (tGFP-tagged) - Human regulator of chromosome condensation 1 (RCC1), transcript variant 4 10 ug
$657.00
SC321255 RCC1 (untagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 4 10 ug
$457.00

Plasmid Map for RC203556

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203556
Circular map for RC203556

file-alt Citations

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