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UAP1 (NM_003115) Human Tagged ORF Clone

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SKU
RC203494
UAP1 (Myc-DDK-tagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $470.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "UAP1 or NM_003115"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UAP1
Synonyms AGX; AGX1; AGX2; SPAG2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203494 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACATTAATGACCTCAAACTCACGTTGTCCAAAGCTGGGCAAGAGCACCTACTACGTTTCTGGAATG
AGCTTGAAGAAGCCCAACAGGTAGAACTTTATGCAGAGCTCCAGGCCATGAACTTTGAGGAGCTGAACTT
CTTTTTCCAAAAGGCCATTGAAGGTTTTAACCAGTCTTCTTACCAAAAGAATGTGGATGCACGAATGGAA
CCTGTGCCTCGAGAGGTATTAGGCAGTGCTACAAGGGATCAAGATCAGCTCCAGGCCTGGGAAAGTGAAG
GACTTTTCCAGATTTCTCAGAATAAAGTAGCAGTTCTTCTTCTAGCTGGTGGGCAGGGGACAAGACTCGG
CGTTGCATATCCTAAGGGGATGTATGATGTTGGTTTGCCATCCCGTAAGACACTTTTTCAGATTCAAGCA
GAGCGTATCCTGAAGCTACAGCAGGTTGCTGAAAAATATTATGGCAACAAATGCATTATTCCATGGTATA
TAATGACCAGTGGCAGAACAATGGAATCTACAAAGGAGTTCTTCACCAAGCACAAGTACTTTGGTTTAAA
AAAAGAGAATGTAATCTTTTTTCAGCAAGGAATGCTCCCCGCCATGAGTTTTGATGGGAAAATTATTTTG
GAAGAGAAGAACAAAGTTTCTATGGCTCCAGATGGGAATGGTGGTCTTTATCGGGCACTTGCAGCCCAGA
ATATTGTGGAGGATATGGAGCAAAGAGGCATTTGGAGCATTCATGTCTATTGTGTTGACAACATATTAGT
AAAAGTGGCAGACCCACGGTTCATTGGATTTTGCATTCAGAAAGGAGCAGACTGTGGAGCAAAGGTGGTA
GAGAAAACGAACCCTACAGAACCAGTTGGAGTGGTTTGCCGAGTGGATGGAGTTTACCAGGTGGTAGAAT
ATAGTGAGATTTCCCTGGCAACAGCTCAAAAACGAAGCTCAGACGGACGACTGCTGTTCAATGCGGGGAA
CATTGCCAACCATTTCTTCACTGTACCATTTCTGAGAGATGTTGTCAATGTTTATGAACCTCAGTTGCAG
CACCATGTGGCTCAAAAGAAGATTCCTTATGTGGATACCCAAGGACAGTTAATTAAGCCAGACAAACCCA
ATGGAATAAAGATGGAAAAATTTGTCTTTGACATCTTCCAGTTTGCAAAGAAGTTTGTGGTATATGAAGT
ATTGCGAGAAGATGAGTTTTCCCCACTAAAGAATGCTGATAGTCAGAATGGGAAAGACAACCCTACTACT
GCAAGGCATGCTTTGATGTCCCTTCATCATTGCTGGGTCCTCAATGCAGGGGGCCATTTCATAGATGAAA
ATGGCTCTCGCCTTCCAGCAATTCCCCGCTTGAAGGATGCCAATGATGTACCAATCCAATGTGAAATCTC
TCCTCTTATCTCCTATGCTGGAGAAGGATTAGAAAGTTATGTGGCAGATAAAGAATTCCATGCACCTCTA
ATCATCGATGAGAATGGAGTTCATGAGCTGGTGAAAAATGGTATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203494 protein sequence
Red=Cloning site Green=Tags(s)

MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSYQKNVDARME
PVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQA
ERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIIL
EEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVV
EKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQ
HHVAQKKIPYVDTQGQLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTT
ARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRLKDANDVPIQCEISPLISYAGEGLESYVADKEFHAPL
IIDENGVHELVKNGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003115
ORF Size 1515 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003115.3, NP_003106.2
RefSeq Size 2344 bp
RefSeq ORF 1518 bp
Locus ID 6675
UniProt ID Q16222, A0A140VKC0
Cytogenetics 1q23.3
Domains UDPGP
Protein Pathways Amino sugar and nucleotide sugar metabolism, Metabolic pathways
MW 57.1 kDa
Summary Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_003115" in other vectors (4)
SKU Description Size Price
RC203494L3 Lenti ORF clone of Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1), Myc-DDK-tagged 10 ug
$770.00
RC203494L4 Lenti ORF clone of Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1), mGFP tagged 10 ug
$770.00
RG203494 UAP1 (tGFP-tagged) - Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1) 10 ug
$489.00 $670.00
SC118166 UAP1 (untagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1) 10 ug
$471.00

Plasmid Map for RC203494

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203494
Circular map for RC203494

file-alt Citations

Reciprocal Regulation Between O-GlcNAcylation and β-Catenin Facilitates Cell Viability and Inhibits Apoptosis in Liver Cancer
Gao, Miao, Liu et al
DNA Cell Biol. (2019)
AI Summary: OriGene products, specifically the β-catenin-Flag expressing plasmid, UAP1 expressing plasmid, and shRNA1/2 sequences for β-catenin knockdown, were used to manipulate gene expression and investigate the reciprocal regulation between O-GlcNAcylation and β-catenin in liver cancer cells.
Snippet: Plasmids and preparation of the lentivirus The β-catenin-Flag expressing plasmid, UDP-N- acetylglucosamine pyrophosphorylase 1 (UAP1) expres- sing plasmid, and the shRNA1/2 sequences for knockdown of β-catenin were all purchased from OriGene (Beijing
Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners
Yu, Boyce, Wands et al
Proc Natl Acad Sci U S A (2012) 109 (13), 4834-9 DOI: 10.1073/pnas.1114356109
Cited by 166
AI Summary: OriGene products contributed to this study by providing the cDNA clones used for expressing the mutant form of UDP-GlcNAc pyrophosphorylase (specifically, the human UAP1 cDNA clone), which enabled metabolic labeling and subsequent photocrosslinking experiments.
Showing 1-2 of 2 papers.
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