ATIC (NM_004044) Human Tagged ORF Clone
SKU
RC203490
ATIC (Myc-DDK-tagged)-Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC)
$551.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ATIC |
| Synonyms | AICAR; AICARFT; HEL-S-70p; IMPCHASE; PURH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004044 |
| ORF Size | 1776 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004044.7 |
| RefSeq Size | 2094 bp |
| RefSeq ORF | 1779 bp |
| Locus ID | 471 |
| UniProt ID | P31939, V9HWH7 |
| Cytogenetics | 2q35 |
| Domains | AICARFT_IMPCHas, MGS |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Metabolic pathways, One carbon pool by folate, Purine metabolism |
| MW | 64.6 kDa |
| Summary | This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. provided by RefSeq, Sep 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004044" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203490L1 | Lenti ORF clone of Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), Myc-DDK-tagged | 10 ug |
$851.00
|
|
| RC203490L2 | Lenti ORF clone of Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), mGFP tagged | 10 ug |
$851.00
|
|
| RC203490L3 | Lenti ORF clone of Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), Myc-DDK-tagged | 10 ug |
$851.00
|
|
| RC203490L4 | Lenti ORF clone of Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), mGFP tagged | 10 ug |
$851.00
|
|
| RG203490 | ATIC (tGFP-tagged) - Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) | 10 ug |
$489.00
$751.00
|
|
| SC320282 | ATIC (untagged)-Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) | 10 ug |
$552.00
|
|
Citations
ATIC facilitates cell growth and migration by upregulating Myc expression in lung adenocarcinoma
Niu, Zeng, Ke et al
Oncol Lett (2022) 23 (4), 131 DOI: 10.3892/ol.2022.13251
Niu, Zeng, Ke et al
Oncol Lett (2022) 23 (4), 131 DOI: 10.3892/ol.2022.13251
Cited by 26
AI Summary: OriGene’s ATIC overexpression vector (cat. no. RC203490) was used to upregulate ATIC expression in lung adenocarcinoma cells, enabling the study of ATIC’s role in promoting cell growth and migration by increasing Myc expression.
Snippet: ltiplicity of infection (MOI) of 5 with the help of polybrene (6 µg/ml). The 3rd generation system was used in following experiments. The ATIC overexpression vector (vector-ATIC;cat. no. RC203490, OriGene Technologies, Inc.) established in pCMV6-Entry vector was used to upregulate ATIC expression. LUAD cells were transfected with vector-ATIC using Lipofectamine 3000 (Invitrogen;Thermo Fische
Showing 1-1 of 1 papers.
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