BIN3 (NM_018688) Human Tagged ORF Clone

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SKU
RC203378
BIN3 (Myc-DDK-tagged)-Human bridging integrator 3 (BIN3)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BIN3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203378 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTGGATTCCTTTTAAGATTGGGCAGCCCAAGAAACAGATTGTGCCCAAAACAGTGGAGAGAGACT
TTGAAAGGGAGTATGGAAAACTTCAGCAGCTGGAAGAGCAGACCCGGAGGCTGCAGAAAGACATGAAGAA
GAGCACCGACGCAGACCTGGCCATGTCAAAATCTGCCGTGAAGATATCCTTGGACTTACTCTCCAATCCC
CTCTGTGAGCAAGACCAGGACCTTCTGAACATGGTGACGGCCCTGGACACGGCCATGAAGCGGATGGATG
CCTTCAATCAGGAAAAGGTGAACCAGATCCAGAAGACTGTGATCGAGCCCTTAAAAAAGTTCGGCAGTGT
CTTCCCGAGCCTCAACATGGCTGTGAAGAGGCGGGAACAGGCCTTGCAGGACTACAGGAGGCTGCAGGCC
AAGGTGGAGAAGTATGAGGAAAAGGAGAAGACGGGGCCAGTGCTGGCCAAGCTCCACCAGGCACGAGAGG
AGCTGCGGCCTGTGCGGGAGGACTTTGAAGCCAAGAACAGGCAGCTGCTGGAGGAGATGCCGCGCTTCTA
CGGCAGCCGCCTCGACTACTTCCAGCCCAGCTTTGAGTCCCTCATCCGAGCTCAGGTTGTGTACTACTCG
GAAATGCACAAGATCTTTGGAGACCTGTCCCATCAGCTTGACCAGCCAGGCCACTCCGATGAGCAGCGGG
AGCGGGAGAACGAGGCCAAACTCAGTGAGCTCCGGGCCCTCTCCATTGTGGCCGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203378 protein sequence
Red=Cloning site Green=Tags(s)

MSWIPFKIGQPKKQIVPKTVERDFEREYGKLQQLEEQTRRLQKDMKKSTDADLAMSKSAVKISLDLLSNP
LCEQDQDLLNMVTALDTAMKRMDAFNQEKVNQIQKTVIEPLKKFGSVFPSLNMAVKRREQALQDYRRLQA
KVEKYEEKEKTGPVLAKLHQAREELRPVREDFEAKNRQLLEEMPRFYGSRLDYFQPSFESLIRAQVVYYS
EMHKIFGDLSHQLDQPGHSDEQRERENEAKLSELRALSIVADD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018688
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018688.3, NP_061158.1
RefSeq Size 1863 bp
RefSeq ORF 762 bp
Locus ID 55909
UniProt ID Q9NQY0
Cytogenetics 8p21.3
Domains BAR
MW 29.7 kDa
Summary The product of this gene is a member of the BAR domain protein family. The encoded protein is comprised solely of a BAR domain which is predicted to form coiled-coil structures and proposed to mediate dimerization, sense and induce membrane curvature, and bind small GTPases. BAR domain proteins have been implicated in endocytosis, intracellular transport, and a diverse set of other processes. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_018688" in other vectors (6)
SKU Description Size Price
RC203378L1 Lenti ORF clone of Human bridging integrator 3 (BIN3), Myc-DDK-tagged 10 ug
$600.00
RC203378L2 Lenti ORF clone of Human bridging integrator 3 (BIN3), mGFP tagged 10 ug
$600.00
RC203378L3 Lenti ORF clone of Human bridging integrator 3 (BIN3), Myc-DDK-tagged 10 ug
$600.00
RC203378L4 Lenti ORF clone of Human bridging integrator 3 (BIN3), mGFP tagged 10 ug
$600.00
RG203378 BIN3 (tGFP-tagged) - Human bridging integrator 3 (BIN3) 10 ug
$489.00 $500.00
SC321244 BIN3 (untagged)-Human bridging integrator 3 (BIN3) 10 ug
$300.00

Plasmid Map for RC203378

Download sequence for

Cloning scheme for RC203378

Circular map for RC203378

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