TM2D3 (NM_025141) Human Tagged ORF Clone

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SKU
RC203360
TM2D3 (Myc-DDK-tagged)-Human TM2 domain containing 3 (TM2D3), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TM2D3
Synonyms BLP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203360 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGAGGGGTGCGCCCGCTGCGGGGCCTCCGCGCCTTGTGTCGCGTGCTGCTCTTCCTCTCGCAGT
TCTGCATTCTGTCGGGCGGTGAAAGTACTGAAATCCCACCTTATGTGATGAAGTGTCCGAGCAATGGTTT
GTGTAGCAGGCTTCCTGCAGACTGTATAGACTGCACAACAAATTTCTCCTGTACCTATGGGAAGCCTGTC
ACTTTTGACTGTGCAGTGAAACCATCTGTTACCTGTGTTGATCAAGACTTCAAATCCCAAAAGAACTTCA
TCATTAACATGACTTGCAGATTTTGCTGGCAGCTTCCTGAAACAGATTACGAGTGTACCAACTCCACCAG
CTGCATGACGGTGTCCTGTCCTCGGCAGCGCTACCCTGCCAACTGCACGGTGCGGGACCACGTCCACTGC
TTGGGTAACCGTACTTTTCCCAAAATGCTATATTGCAATTGGACTGGAGGCTATAAGTGGTCTACGGCTC
TGGCTCTAAGCATCACCCTCGGTGGGTTTGGAGCAGACCGTTTCTACCTGGGCCAGTGGCGGGAAGGCCT
CGGCAAGCTCTTCAGCTTCGGTGGCCTGGGAATATGGACGCTGATAGACGTCCTGCTCATTGGAGTTGGC
TATGTTGGACCAGCAGATGGCTCTTTGTACATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203360 protein sequence
Red=Cloning site Green=Tags(s)

MAGGVRPLRGLRALCRVLLFLSQFCILSGGESTEIPPYVMKCPSNGLCSRLPADCIDCTTNFSCTYGKPV
TFDCAVKPSVTCVDQDFKSQKNFIINMTCRFCWQLPETDYECTNSTSCMTVSCPRQRYPANCTVRDHVHC
LGNRTFPKMLYCNWTGGYKWSTALALSITLGGFGADRFYLGQWREGLGKLFSFGGLGIWTLIDVLLIGVG
YVGPADGSLYI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025141
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025141.3
RefSeq Size 1343 bp
RefSeq ORF 666 bp
Locus ID 80213
UniProt ID Q9BRN9
Cytogenetics 15q26.3
Domains TM2
Protein Families Druggable Genome, Transmembrane
MW 24.4 kDa
Summary The protein encoded by this gene contains a structural module related to that of the seven transmembrane domain G protein-coupled receptor superfamily. This protein has sequence and structural similarities to the beta-amyloid binding protein (BBP), but, unlike BBP, it does not regulate a response to beta-amyloid peptide. This protein may have regulatory roles in cell death or proliferation signal cascades. Several alternatively spliced transcript variants of this gene are described but the full length nature of some variants has not been determined. Multiple polyadenylation sites have been found in this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_025141" in other vectors (4)
SKU Description Size Price
RC203360L3 Lenti ORF clone of Human TM2 domain containing 3 (TM2D3), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC203360L4 Lenti ORF clone of Human TM2 domain containing 3 (TM2D3), transcript variant 2, mGFP tagged 10 ug
$600.00
RG203360 TM2D3 (tGFP-tagged) - Human TM2 domain containing 3 (TM2D3), transcript variant 2 10 ug
$489.00 $500.00
SC110640 TM2D3 (untagged)-Human TM2 domain containing 3 (TM2D3), transcript variant 2 10 ug
$300.00

Plasmid Map for RC203360

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Cloning scheme for RC203360

Circular map for RC203360

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