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XAB2 (NM_020196) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC203353
XAB2 (Myc-DDK-tagged)-Human XPA binding protein 2 (XAB2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$589.00 $783.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol XAB2
Synonyms HCNP; HCRN; NTC90; SYF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203353 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGTGATGGCGCGACTCTCGCGGCCCGAGCGGCCGGACCTTGTCTTCGAGGAAGAGGACCTCCCCT
ATGAGGAGGAAATCATGCGGAACCAATTCTCTGTCAAATGCTGGCTTCGCTACATCGAGTTCAAACAGGG
CGCCCCGAAGCCCAGGCTCAATCAGCTATACGAGCGGGCACTCAAGCTGCTGCCCTGCAGCTACAAACTC
TGGTACCGATACCTGAAGGCGCGTCGGGCACAGGTGAAGCATCGCTGTGTGACCGACCCTGCCTATGAAG
ATGTCAACAACTGTCATGAGAGGGCCTTTGTGTTCATGCACAAGATGCCTCGTCTGTGGCTAGATTACTG
CCAGTTCCTCATGGACCAGGGGCGCGTCACACACACCCGCCGCACCTTCGACCGTGCCCTCCGGGCACTG
CCCATCACGCAGCACTCTCGAATTTGGCCCCTGTATCTGCGCTTCCTGCGCTCACACCCACTGCCTGAGA
CAGCTGTGCGAGGCTATCGGCGCTTCCTCAAGCTGAGTCCTGAGAGTGCAGAGGAGTACATTGAGTACCT
CAAGTCAAGTGACCGGCTGGATGAGGCCGCCCAGCGCCTGGCCACCGTGGTGAACGACGAGCGTTTCGTG
TCTAAGGCCGGCAAGTCCAACTACCAGCTGTGGCACGAGCTGTGCGACCTCATCTCCCAGAATCCGGACA
AGGTACAGTCCCTCAATGTGGACGCCATCATCCGCGGGGGCCTCACCCGCTTCACCGACCAGCTGGGCAA
GCTCTGGTGTTCTCTCGCCGACTACTACATCCGCAGCGGCCATTTCGAGAAGGCTCGGGACGTGTACGAG
GAGGCCATCCGGACAGTGATGACCGTGCGGGACTTCACACAGGTGTTTGACAGCTACGCCCAGTTCGAGG
AGAGCATGATCGCTGCAAAGATGGAGACCGCCTCGGAGCTGGGGCGCGAGGAGGAGGATGATGTGGACCT
GGAGCTGCGCCTGGCCCGCTTCGAGCAGCTCATCAGCCGGCGGCCCCTGCTCCTCAACAGCGTCTTGCTG
CGCCAAAACCCACACCACGTGCACGAGTGGCACAAGCGTGTCGCCCTGCACCAGGGCCGCCCCCGGGAGA
TCATCAACACCTACACAGAGGCTGTGCAGACGGTGGACCCCTTCAAGGCCACAGGCAAGCCCCACACTCT
GTGGGTGGCGTTTGCCAAGTTTTATGAGGACAACGGACAGCTGGACGATGCCCGTGTCATCCTGGAGAAG
GCCACCAAGGTGAACTTCAAGCAGGTGGATGACCTGGCAAGCGTGTGGTGTCAGTGCGGAGAGCTGGAGC
TCCGACACGAGAACTACGATGAGGCCTTGCGGCTGCTGCGAAAGGCCACGGCGCTGCCTGCCCGCCGGGC
CGAGTACTTTGATGGTTCAGAGCCCGTGCAGAACCGCGTGTACAAGTCACTGAAGGTCTGGTCCATGCTC
GCCGACCTGGAGGAGAGCCTCGGCACCTTCCAGTCCACCAAGGCCGTGTACGACCGCATCCTGGACCTGC
GTATCGCAACACCCCAGATCGTCATCAACTATGCCATGTTCCTGGAGGAGCACAAGTACTTCGAGGAGAG
CTTCAAGGCGTACGAGCGCGGCATCTCGCTGTTCAAGTGGCCCAACGTGTCCGACATCTGGAGCACCTAC
CTGACCAAATTCATTGCCCGCTATGGGGGCCGCAAGCTGGAGCGGGCACGGGACCTGTTTGAACAGGCTC
TGGACGGCTGCCCCCCAAAATATGCCAAGACCTTGTACCTGCTGTACGCACAGCTGGAGGAGGAGTGGGG
CCTGGCCCGGCATGCCATGGCCGTGTACGAGCGTGCCACCAGGGCCGTGGAGCCCGCCCAGCAGTATGAC
ATGTTCAACATCTACATCAAGCGGGCGGCCGAGATCTATGGGGTCACCCACACCCGCGGCATCTACCAGA
AGGCCATTGAGGTGCTGTCGGACGAGCACGCGCGTGAGATGTGCCTGCGGTTTGCAGACATGGAGTGCAA
GCTCGGGGAGATTGACCGCGCCCGGGCCATCTACAGCTTCTGCTCCCAGATCTGTGACCCCCGGACGACC
GGCGCGTTCTGGCAGACGTGGAAGGACTTTGAGGTCCGGCATGGCAATGAGGACACCATCAAGGAAATGC
TGCGTATCCGGCGCAGCGTGCAGGCCACGTACAACACGCAGGTCAACTTCATGGCCTCGCAGATGCTCAA
GGTCTCGGGCAGTGCCACGGGCACCGTGTCTGACCTGGCCCCTGGGCAGAGTGGCATGGACGACATGAAG
CTGCTGGAACAGCGGGCAGAGCAGCTGGCGGCTGAGGCGGAGCGTGACCAGCCCTTGCGCGCCCAGAGCA
AGATCCTGTTCGTGAGGAGTGACGCCTCCCGGGAGGAGCTGGCAGAGCTGGCACAGCAGGTCAACCCCGA
GGAGATCCAGCTGGGCGAGGACGAGGACGAGGACGAGATGGACCTGGAGCCCAACGAGGTTCGGCTGGAG
CAGCAGAGCGTGCCAGCCGCAGTGTTTGGGAGCCTGAAGGAAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203353 protein sequence
Red=Cloning site Green=Tags(s)

MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERALKLLPCSYKL
WYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFLMDQGRVTHTRRTFDRALRAL
PITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESAEEYIEYLKSSDRLDEAAQRLATVVNDERFV
SKAGKSNYQLWHELCDLISQNPDKVQSLNVDAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYE
EAIRTVMTVRDFTQVFDSYAQFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLL
RQNPHHVHEWHKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEK
ATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRVYKSLKVWSML
ADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKAYERGISLFKWPNVSDIWSTY
LTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYAQLEEEWGLARHAMAVYERATRAVEPAQQYD
MFNIYIKRAAEIYGVTHTRGIYQKAIEVLSDEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTT
GAFWQTWKDFEVRHGNEDTIKEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMK
LLEQRAEQLAAEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLE
QQSVPAAVFGSLKED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020196
ORF Size 2565 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020196.3
RefSeq Size 2682 bp
RefSeq ORF 2568 bp
Locus ID 56949
UniProt ID Q9HCS7
Cytogenetics 19p13.2
Domains HAT
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Spliceosome
MW 100 kDa
Summary Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28502770, PubMed:28076346). Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing (PubMed:10944529, PubMed:17981804).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_020196" in other vectors (6)
SKU Description Size Price
RC203353L1 Lenti ORF clone of Human XPA binding protein 2 (XAB2), Myc-DDK-tagged 10 ug
$1,083.00
RC203353L2 Lenti ORF clone of Human XPA binding protein 2 (XAB2), mGFP tagged 10 ug
$1,083.00
RC203353L3 Lenti ORF clone of Human XPA binding protein 2 (XAB2), Myc-DDK-tagged 10 ug
$1,083.00
RC203353L4 Lenti ORF clone of Human XPA binding protein 2 (XAB2), mGFP tagged 10 ug
$1,083.00
RG203353 XAB2 (tGFP-tagged) - Human XPA binding protein 2 (XAB2) 10 ug
$983.00
SC319216 XAB2 (untagged)-Human XPA binding protein 2 (XAB2) 10 ug
$784.00

Plasmid Map for RC203353

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203353
Circular map for RC203353

file-alt Citations

XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE
Hou, Li, Zhang et al
Cell Death Dis (2016) 7 (10), e2409 DOI: 10.1038/cddis.2016.313
AI Summary: OriGene’s XAB2 construct was used in this study to investigate the role of XAB2 in mitotic cell cycle progression and its transcriptional regulation of CENPE.
Snippet: cancer therapy. Top of page Materials and Methods. Constructs and antibodies.XAB2 construct was purchased from Origene and re-cloned into modified pcDNA3.1 vector(Promega, USA) containing HA tag at the 5 end. A 1355 ... Cite SaveSaving
Showing 1-1 of 1 papers.
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