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SEPSECS (NM_016955) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC203211
SEPSECS (Myc-DDK-tagged)-Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "SEPSECS or NM_016955"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SEPSECS
Synonyms LP; PCH2D; SLA; SLA/LP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203211 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCACCTCATACGGCTGCTTCTGGAGAAGGTTCATTCATGGCATTGGACGATCCGGTGATATTTCTG
CTGTGCAACCAAAAGCTGCAGGCTCTAGCCTTTTGAACAAAATTACCAATTCTTTGGTCCTGGACATTAT
AAAGCTGGCTGGTGTCCATACAGTAGCCAACTGCTTTGTAGTTCCTATGGCAACTGGTATGAGTCTAACT
CTGTGTTTCTTAACATTACGACACAAAAGACCAAAGGCAAAGTATATTATATGGCCACGAATAGACCAGA
AGTCCTGCTTTAAATCCATGATCACTGCAGGTTTTGAGCCTGTGGTGATAGAAAATGTTTTGGAAGGTGA
CGAGCTGCGTACAGACCTGAAAGCAGTGGAGGCTAAAGTCCAGGAACTTGGGCCTGATTGCATTCTGTGT
ATTCATTCTACTACATCCTGTTTTGCTCCAAGGGTGCCTGATAGATTAGAAGAACTGGCTGTGATTTGTG
CTAATTATGACATTCCACATATAGTTAATAATGCTTATGGAGTGCAGTCTTCAAAGTGTATGCATCTCAT
TCAGCAGGGGGCTCGAGTTGGTAGAATAGATGCTTTTGTTCAGAGCTTGGACAAAAATTTTATGGTTCCA
GTAGGTGGTGCTATAATTGCTGGCTTTAATGATTCATTCATTCAGGAAATCAGCAAGATGTATCCAGGAA
GAGCTTCAGCTTCACCTTCTTTAGATGTCCTTATTACTTTATTGTCACTTGGATCAAATGGCTATAAGAA
GCTACTAAAAGAAAGAAAGGAAATGTTTTCATATTTGTCCAACCAAATAAAGAAGTTGTCAGAAGCCTAC
AATGAAAGACTGTTGCATACACCTCACAATCCCATATCTTTAGCTATGACACTTAAAACACTAGATGAAC
ACCGTGACAAAGCTGTCACTCAGCTTGGCTCGATGCTTTTTACCAGACAGGTTTCTGGAGCCAGGGTTGT
GCCTCTTGGGTCCATGCAAACTGTGAGTGGCTATACTTTCAGAGGCTTTATGTCACATACAAATAATTAC
CCTTGTGCTTACCTCAATGCTGCATCAGCCATCGGAATGAAGATGCAGGATGTGGACCTGTTCATAAAGA
GACTTGACAGGTGTTTAAAGGCAGTAAGAAAAGAACGAAGTAAAGAGAGTGATGACAATTATGACAAAAC
TGAAGATGTGGATATTGAAGAAATGGCTTTAAAACTAGATAATGTACTTCTTGACACATACCAGGATGCT
TCTTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203211 protein sequence
Red=Cloning site Green=Tags(s)

MSTSYGCFWRRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVANCFVVPMATGMSLT
LCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCILC
IHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVP
VGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAY
NERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNY
PCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRKERSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQDA
SS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016955
ORF Size 1266 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016955.2, NP_058651.2
RefSeq Size 5645 bp
RefSeq ORF 1506 bp
Locus ID 51091
UniProt ID Q9HD40
Cytogenetics 4p15.2
Protein Pathways Aminoacyl-tRNA biosynthesis
MW 46.9 kDa
Summary The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).provided by RefSeq, Mar 2011
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"NM_016955" in other vectors (4)
SKU Description Size Price
RC203211L3 Lenti ORF clone of Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), Myc-DDK-tagged 10 ug
$757.00
RC203211L4 Lenti ORF clone of Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), mGFP tagged 10 ug
$757.00
RG203211 SEPSECS (tGFP-tagged) - Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS) 10 ug
$489.00 $657.00
SC114127 SEPSECS (untagged)-Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS) 10 ug
$457.00

Plasmid Map for RC203211

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203211
Circular map for RC203211

file-alt Citations

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