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SEPSECS (NM_016955) Human Untagged Clone

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SKU
SC114127
SEPSECS (untagged)-Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS)
  $457.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SEPSECS
Synonyms LP; PCH2D; SLA; SLA/LP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_016955, the custom clone sequence may differ by one or more nucleotides


ATGAACCGCGAGAGCTTCGCGGCGGGAGAGCGGCTGGTGTCGCCGGCTTACGTGCGGCAGGGCTGTGAGG
CCCGCCGCTCGCATGAGCACCTCATACGGCTGCTTCTGGAGAAGGGCAAGTGTCCAGAGAATGGCTGGGA
TGAAAGTACACTTGAACTCTTTTTACATGAACTTGCAATCATGGACAGCAACAATTTCTTAGGCAATTGT
GGTGTGGGAGAAAGGGAAGGGAGAGTGGCATCCGCACTGGTTGCTCGTCGTCATTACAGGTTCATTCATG
GCATTGGACGATCCGGTGATATTTCTGCTGTGCAACCAAAAGCTGCAGGCTCTAGCCTTTTGAACAAAAT
TACCAATTCTTTGGTCCTGGACATTATAAAGCTGGCTGGTGTCCATACAGTAGCCAACTGCTTTGTAGTT
CCTATGGCAACTGGTATGAGTCTAACTCTGTGTTTCTTAACATTACGACACAAAAGACCAAAGGCAAAGT
ATATTATATGGCCACGAATAGACCAGAAGTCCTGCTTTAAATCCATGATCACTGCAGGTTTTGAGCCTGT
GGTGATAGAAAATGTTTTGGAAGGTGACGAGCTGCGTACAGACCTGAAAGCAGTGGAGGCTAAAGTCCAG
GAACTTGGGCCTGATTGCATTCTGTGTATTCATTCTACTACATCCTGTTTTGCTCCAAGGGTGCCTGATA
GATTAGAAGAACTGGCTGTGATTTGTGCTAATTATGACATTCCACATATAGTTAATAATGCTTATGGAGT
GCAGTCTTCAAAGTGTATGCATCTCATTCAGCAGGGGGCTCGAGTTGGTAGAATAGATGCTTTTGTTCAG
AGCTTGGACAAAAATTTTATGGTTCCAGTAGGTGGTGCTATAATTGCTGGCTTTAATGATTCATTCATTC
AGGAAATCAGCAAGATGTATCCAGGAAGAGCTTCAGCTTCACCTTCTTTAGATGTCCTTATTACTTTATT
GTCACTTGGATCAAATGGCTATAAGAAGCTACTAAAAGAAAGAAAGGAAATGTTTTCATATTTGTCCAAC
CAAATAAAGAAGTTGTCAGAAGCCTACAATGAAAGACTGTTGCATACACCTCACAATCCCATATCTTTAG
CTATGACACTTAAAACACTAGATGAACACCGTGACAAAGCTGTCACTCAGCTTGGCTCGATGCTTTTTAC
CAGACAGGTTTCTGGAGCCAGGGTTGTGCCTCTTGGGTCCATGCAAACTGTGAGTGGCTATACTTTCAGA
GGCTTTATGTCACATACAAATAATTACCCTTGTGCTTACCTCAATGCTGCATCAGCCATCGGAATGAAGA
TGCAGGATGTGGACCTGTTCATAAAGAGACTTGACAGGTGTTTAAAGGCAGTAAGAAAAGAACGAAGTAA
AGAGAGTGATGACAATTATGACAAAACTGAAGATGTGGATATTGAAGAAATGGCTTTAAAACTAGATAAT
GTACTTCTTGACACATACCAGGATGCTTCTTCATGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_016955 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGTGTGTCTGGCGTG
TGGAGCTAGTGCTCCCGCTTATTCGCCACTGTCGCCACCGCTGTCATGAACCGCGAGAGC
TTCGCGGCGGGAGAGCGGCTGGTGTCGCCGGCTTACGTGCGGCAGGGCTGTGAGGCCCGC
CGCTCGCATGAGCACCTCATACGGCTGCTTCTGGAGAAGGGCAAGTGTCCAGAGAATGGC
TGGGATGAAAGTACACTTGAACTCTTTTTACATGAACTTGCAATCATGGACAGCAACAAT
TTCTTAGGCAATTGTGGTGTGGGAGAAAGGGAAGGGAGAGTGGCATCCGCACTGGTTGCT
CGTCGTCATTACAGGTTCATTCATGGCATTGGACGATCCGGTGATATTTCTGCTGTGCAA
CCAAAAGCTGCAGGCTCTAGCCTTTTGAACAAAATTACCAATTCTTTGGTCCTGGACATT
ATAAAGCTGGCTGGTGTCCATACAGTAGCCAACTGCTTTGTAGTTCCTATGGCAACTGGT
ATGAGTCTAACTCTGTGTTTCTTAACATTACGACACAAAAGACCAAAGGCAAAGTATATT
ATATGGCCACGAATAGACCAGAAGTCCTGCTTTAAATCCATGATCACTGCAGGTTTTGAG
CCTGTGGTGATAGAAAATGGTTTGGAAGGTGACGAGCTGCGTACAGACCTGAAAGCAGTG
GAGGCTAAAGTCCAGGAACTTGGGCCTGATTGCATTCTGTGTATTCATTCTACTACATCC
TGTTNTGCTCCAAGGGTGCCTGATAGATANAAAGACTGGCTGTGATTNGTGCTANTATGA
CATTCACATATAGNTAATATGCTTATGGAGTGCAGTCTTCAAGTGTATGCATCTCATCAG
CAGGGGCTCGAGTGGTAGATGATGCTTTGTCAAGCTGNCANNATTTTGGNTCGTAGN
Restriction Sites NotI-NotI |
ACCN NM_016955
Insert Size 1269 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016955.1, NP_058651.1
RefSeq Size 1269 bp
RefSeq ORF 1269 bp
Locus ID 51091
UniProt ID Q9HD40
Cytogenetics 4p15.2
Protein Pathways Aminoacyl-tRNA biosynthesis
Summary The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).provided by RefSeq, Mar 2011
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Documents
Resources
"NM_016955" in other vectors (4)
SKU Description Size Price
RC203211 SEPSECS (Myc-DDK-tagged)-Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS) 10 ug
$289.00 $457.00
RC203211L3 Lenti ORF clone of Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), Myc-DDK-tagged 10 ug
$757.00
RC203211L4 Lenti ORF clone of Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), mGFP tagged 10 ug
$757.00
RG203211 SEPSECS (tGFP-tagged) - Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS) 10 ug
$489.00 $657.00

file-alt Citations

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