YIPF5 (NM_030799) Human Tagged ORF Clone

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SKU
RC203192
YIPF5 (Myc-DDK-tagged)-Human Yip1 domain family, member 5 (YIPF5), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol YIPF5
Synonyms FinGER5; SB140; SMAP-5; SMAP5; YIP1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203192 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGGCTTTGAAAACTTAAACACGGATTTCTACCAGACAAGTTACAGCATCGATGATCAGTCACAGC
AGTCCTATGATTATGGAGGAAGTGGAGGACCCTATAGCAAACAGTATGCTGGCTATGACTATTCGCAGCA
AGGCAGATTTGTCCCTCCAGACATGATGCAGCCACAACAGCCATACACCGGGCAGATTTACCAGCCAACT
CAGGCATATACTCCAGCTTCACCTCAGCCTTTCTATGGAAACAACTTTGAGGATGAGCCACCTTTATTAG
AAGAGTTAGGTATCAATTTTGACCACATCTGGCAAAAAACACTAACAGTATTACATCCGTTAAAAGTAGC
AGATGGCAGCATCATGAATGAAACTGATTTGGCAGGTCCAATGGTTTTTTGCCTTGCTTTTGGAGCCACA
TTGCTACTGGCTGGCAAAATCCAGTTTGGCTATGTATACGGGATCAGTGCAATTGGATGTCTAGGAATGT
TTTGTTTATTAAACTTAATGAGTATGACAGGTGTTTCATTTGGTTGTGTGGCAAGTGTCCTTGGATATTG
TCTTCTGCCCATGATCCTACTTTCCAGCTTTGCAGTGATATTTTCTTTGCAAGGAATGGTAGGAATCATT
CTCACTGCTGGGATTATTGGATGGTGTAGTTTTTCTGCTTCCAAAATATTTATTTCTGCATTAGCCATGG
AAGGACAGCAACTTTTAGTAGCATATCCTTGCGCTTTGTTATATGGAGTCTTTGCCCTGATTTCCGTCTT
T


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203192 protein sequence
Red=Cloning site Green=Tags(s)

MSGFENLNTDFYQTSYSIDDQSQQSYDYGGSGGPYSKQYAGYDYSQQGRFVPPDMMQPQQPYTGQIYQPT
QAYTPASPQPFYGNNFEDEPPLLEELGINFDHIWQKTLTVLHPLKVADGSIMNETDLAGPMVFCLAFGAT
LLLAGKIQFGYVYGISAIGCLGMFCLLNLMSMTGVSFGCVASVLGYCLLPMILLSSFAVIFSLQGMVGII
LTAGIIGWCSFSASKIFISALAMEGQQLLVAYPCALLYGVFALISVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030799
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030799.9
RefSeq Size 3212 bp
RefSeq ORF 774 bp
Locus ID 81555
UniProt ID Q969M3
Cytogenetics 5q31.3
Domains Yip1
Protein Families Transmembrane
MW 28 kDa
Summary Plays a role in transport between endoplasmic reticulum and Golgi.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030799" in other vectors (6)
SKU Description Size Price
RC203192L1 Lenti ORF clone of Human Yip1 domain family, member 5 (YIPF5), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC203192L2 Lenti ORF clone of Human Yip1 domain family, member 5 (YIPF5), transcript variant 2, mGFP tagged 10 ug
$600.00
RC203192L3 Lenti ORF clone of Human Yip1 domain family, member 5 (YIPF5), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC203192L4 Lenti ORF clone of Human Yip1 domain family, member 5 (YIPF5), transcript variant 2, mGFP tagged 10 ug
$600.00
RG203192 YIPF5 (tGFP-tagged) - Human Yip1 domain family, member 5 (YIPF5), transcript variant 2 10 ug
$489.00 $500.00
SC321529 YIPF5 (untagged)-Human Yip1 domain family, member 5 (YIPF5), transcript variant 2 10 ug
$300.00

Plasmid Map for RC203192

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Cloning scheme for RC203192

Circular map for RC203192

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