PDE6D (NM_002601) Human Tagged ORF Clone
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PDE6D |
| Synonyms | JBTS22; PDED |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002601 |
| ORF Size | 450 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002601.4 |
| RefSeq Size | 1214 bp |
| RefSeq ORF | 453 bp |
| Locus ID | 5147 |
| UniProt ID | O43924, Q6IB24 |
| Cytogenetics | 2q37.1 |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 17.4 kDa |
| Summary | This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. provided by RefSeq, Mar 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002601" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203172L1 | Lenti ORF clone of Human phosphodiesterase 6D, cGMP-specific, rod, delta (PDE6D), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC203172L2 | Lenti ORF clone of Human phosphodiesterase 6D, cGMP-specific, rod, delta (PDE6D), mGFP tagged | 10 ug |
$525.00
|
|
| RC203172L3 | Lenti ORF clone of Human phosphodiesterase 6D, cGMP-specific, rod, delta (PDE6D), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC203172L4 | Lenti ORF clone of Human phosphodiesterase 6D, cGMP-specific, rod, delta (PDE6D), mGFP tagged | 10 ug |
$525.00
|
|
| RG203172 | PDE6D (tGFP-tagged) - Human phosphodiesterase 6D, cGMP-specific, rod, delta (PDE6D) | 10 ug |
$425.00
|
|
| SC118535 | PDE6D (untagged)-Human phosphodiesterase 6D, cGMP-specific, rod, delta (PDE6D) | 10 ug |
$225.00
|
|
Citations
The Delta Subunit of Rod-Specific Photoreceptor cGMP Phosphodiesterase (PDE6D) Contributes to Hepatocellular Carcinoma Progression
Dietrich, Hellerbrand, Bosserhoff
Cancers (Basel) (2019) 11 (3) DOI: 10.3390/cancers11030398
Dietrich, Hellerbrand, Bosserhoff
Cancers (Basel) (2019) 11 (3) DOI: 10.3390/cancers11030398
A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium
Thomas, Wright, Le Corre et al
Hum. Mutat. (2013)
Thomas, Wright, Le Corre et al
Hum. Mutat. (2013)
Cited by 129
Identification of PDE6D as a molecular target of anecortave acetate via a methotrexate-anchored yeast three-hybrid screen
Shepard, Conrow, Pang et al
ACS Chem. Biol. (2013) 8 (3), 549-58 DOI: 10.1021/cb300296m
Shepard, Conrow, Pang et al
ACS Chem. Biol. (2013) 8 (3), 549-58 DOI: 10.1021/cb300296m
Cited by 15
AI Summary: In this study, OriGene’s His-tagged DHFR protein was used in coimmunoprecipitation experiments to confirm the binding interaction between anecortave acetate derivatives and their molecular target, PDE6D.
Snippet: Incubation in the presence of A1-Mtx revealed a band corresponding to the size of the His-tagged DHFR ( 20 kD), which could be competed off in elutions incubated with excess AdesA (500 μM) (Figure 5). His-DHFR detected in the elution fractions of AdesA competed with A1
Showing 1-3 of 3 papers.
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