NDUFB6 (NM_002493) Human Tagged ORF Clone
SKU
RC203155
NDUFB6 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NDUFB6 |
| Synonyms | B17; CI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002493 |
| ORF Size | 384 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002493.5 |
| RefSeq Size | 873 bp |
| RefSeq ORF | 387 bp |
| Locus ID | 4712 |
| UniProt ID | O95139 |
| Cytogenetics | 9p21.1 |
| Protein Families | Transmembrane |
| Protein Pathways | Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease |
| MW | 15.5 kDa |
| Summary | The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. provided by RefSeq, Jan 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002493" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203155L1 | Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC203155L2 | Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged | 10 ug |
$450.00
|
|
| RC203155L3 | Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC203155L4 | Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged | 10 ug |
$450.00
|
|
| RG203155 | NDUFB6 (tGFP-tagged) - Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1 | 10 ug |
$489.00
|
|
| SC118621 | NDUFB6 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1 | 10 ug |
$150.00
|
|
Citations
YME1L controls the accumulation of respiratory chain subunits and is required for apoptotic resistance, cristae morphogenesis, and cell proliferation
Stiburek, Cesnekova, Kostkova et al
Mol. Biol. Cell (2012) 23 (6), 1010-23 DOI: 10.1091/mbc.E11-08-0674
Stiburek, Cesnekova, Kostkova et al
Mol. Biol. Cell (2012) 23 (6), 1010-23 DOI: 10.1091/mbc.E11-08-0674
Cited by 3
AI Summary: OriGene products, specifically the YME1L shRNA plasmid and YME1L expression constructs, were used to stably knock down and re-express YME1L in human cells, enabling the authors to elucidate YME1L's roles in mitochondrial function, cristae morphology, and respiratory chain subunit regulation.
Snippet: membrane proteins. Here we investigate the cellular activities of YME1L, the human orthologue of the Yme1 subunit of the yeast i‑AAA complex, using stable short hairpin RNA knockdown and expression experiments. Human
Showing 1-1 of 1 papers.
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