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NDUFB6 (NM_002493) Human Untagged Clone

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SKU
SC118621
NDUFB6 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NDUFB6
Synonyms B17; CI
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_002493 edited
ATGACGGGGTACACTCCGGATGAGAAACTGCGGCTGCAGCAGCTGCGAGAGCTGAGAAGG
CGATGGCTGAAGGACCAGGAGCTGAGCCCTCGGGAGCCGGTGCTGCCCCCACAGAAGATG
GGGCCTATGGAGAAATTCTGGAATAAATTTTTGGAGAATAAATCCCCTTGGAGGAAAATG
GTCCATGGGGTATACAAAAAGAGTATCTTTGTTTTCACTCATGTACTTGTACCTGTCTGG
ATTATTCATTATTACATGAAGTATCATGTTTCTGAAAAACCATATGGCATAGTTGAAAAG
AAGTCCAGAATATTCCCTGGTGATACAATTCTGGAGACTGGAGAAGTAATTCCACCAATG
AAAGAATTTCCTGATCAACATCATTAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002493 unedited
GAGTCGGATTTGTAACCGACTTTACTATAGGGCGGCACGCGCAATTCGGCACGAGGCTTT
GCAGAGCGGGAGCGCGCTTAAGTAACTAGTCCGTAGTTCGAGGGTGCGCCGTGTCCTTTT
GCGTTGGTACCAGCGGCGACATGACGGGGTACACTCCGGATGAGAAACTGCGGCTGCAGC
AGCTGCGAGAGCTGAGAAGGCGATGGCTGAAGGACCAGGAGCTGAGCCCTCGGGAGCCGG
TGCTGCCCCCACAGAAGATGGGGCCTATGGAGAAATTCTGGAATAAATTTTTGGAGAATA
AATCCCCTTGGAGGAAAATGGTCCATGGGGTATACAAAAAGAGTATCTTTGTTTTCACTC
ATGTACTTGTACCTGTCTGGATTATTCATTATTACATGAAGTATCATGTTTCTGAAAAAC
CATATGGCATAGTTGAAAAGAAGTCCAGAATATTCCCTGGTGATACAATTCTGGAGACTG
GAGAAGTAATTCCACCAATGAAAGAATTTCCTGATCAACATCATTAAAGATTATGTAAAA
AGTTAAAAGGCTTATGAGCCTAAGTTTGTTCCTATATTACCATATTTACTGAATTTTCTG
GAAAAGTAACTTTAATAAAGTTTAATCTCAGAAAANAAAANNNNAANNNAAANNNANNNN
NNNNANAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCGCCTCTAAATTGCGGCCG
GGGACTTAGTTGTTCCTGAACCAGATCCGGGTTGAATCCTTGTACCCCTTCCCCAGGGCC
TTTCCGGGCCTGGAAATTTGCCCTTCCGTGCCCCCCAGCCTTGCCCTAAAAAAATAAGTG
CATAATTTTGCCGCACTGGTGCCCTTCTATAAATAAGGGGTGGAGGGGGTGTGAATGGAA
CCAAGGGCAATTTTGAAAACACACC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002493 unedited
GGGGTGGGTGTGTATTAAATAAAGCTAACTTATCTAANCCNNGGCNTCCGTTATTANAAC
TTTCTATCAAGATTTTCTTTTNCCANNAAATTNCAGTNAATTATTGGTAATATAGGGAAC
AAACTTAGGCTCATAAGCCTTTTAACTTTTTACATAATCTTTAATGATGTTGATCAGGAA
ATTCTTTCATTGGTGGAATTACTTCTCCAGTCTCCAGAATTGTATCACCAGGGAATATTC
TGGACTTCTTTTCAACTATGCCATATGGTTTTTCAGAAACATGATACTTCATGTAATAAT
GAATAATCCAGACAGGTACAAGTACATGAGTGAAAACAAAGATACTCTTTTTGTATACCC
CATGGACCATTTTCCTCCAAGGGGATTTATTCTCCAAAAATTTATTCCAGAATTTCTCCA
TAGGCCCCATCTTCTGTGGGGGCAGCACCGGCTCCCGAGGGCTCAGCTCCTGGTCCTTCA
GCCATCGCCTTCTCAGCTCTCGCAGCTGCTGCAGCCGCAGTTTCTCATCCGGAGTGTACC
CCGTCATGTCGCCGCTGGTACCAACGCAAAAGGACACGGCGCACCCTCGAACTACGGACT
AGTTACTTAAGCGCGCTCCCGCTCTGCAAAGCCTCGTGCCGAATTCGCGGCCGCCCTATA
GTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCC
CACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGGCGGGTTATTACGACATTTTGGA
AAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGGTGGAGAC
TTGGAAATCCCCGTGAATCAAACCGCTATCCACCCCCATTGGTGTACTGGCCAAAACCCC
ATTACCATGGTTAATAGCGAATGACTAATACCTTTCATGTTCCTGCCCAATAAGTAAAGT
CCCGCTAAGTN
Restriction Sites NotI-NotI |
ACCN NM_002493
Insert Size 650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002493.3, NP_002484.1
RefSeq Size 862 bp
RefSeq ORF 387 bp
Locus ID 4712
UniProt ID O95139
Cytogenetics 9p21.1
Protein Families Transmembrane
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Summary The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. provided by RefSeq, Jan 2011
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_002493" in other vectors (6)
SKU Description Size Price
RC203155 NDUFB6 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$150.00
RC203155L1 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC203155L2 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$450.00
RC203155L3 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC203155L4 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$450.00
RG203155 NDUFB6 (tGFP-tagged) - Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa (NDUFB6), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00

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