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RWDD2A (NM_033411) Human Tagged ORF Clone

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SKU
RC203103
RWDD2A (Myc-DDK-tagged)-Human RWD domain containing 2A (RWDD2A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RWDD2A
Synonyms dJ747H23.2; RWDD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203103 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCTTCGGTGAAAGAAAGCCTTCAGCTTCAGCTACTGGAGATGGAAATGCTGTTTTCTATGTTTC
CTAACCAAGGAGAAGTAAAACTTGAAGATGTAAATGCCCTGACGAATATAAAGAGGTATTTGGAAGGCAC
AAGGGAGGCGCTGCCACCAAAAATCGAATTTGTAATTACACTCCAGATTGAAGAGCCCAAGGTGAAAATT
GATTTGCAAGTGACCATGCCTCACAGCTACCCCTATTTAGCATTGCAGCTGTTTGGACGGTCATCTGAAC
TTGACAGACATCAGCAGCTACTTCTCAACAAAGGTCTCACTTCTTACATAGGGACTTTTGATCCAGGTGA
GCTCTGTGTATGTGCAGCAATCCAGTGGCTACAGGACAACAGTGCATCTTATTTCCTGAACAGAAAGCTT
GTATATGAACCATCTACACAAGCAAAGCCAGTCAAGAACACATTCCTCCGAATGTGGATCTACAGTCACC
ATATATATCAGCAGGACCTAAGGAAAAAGATTTTGGATGTTGGGAAAAGGTTAGATGTGACTGGATTTTG
CATGACAGGAAAGCCTGGTATAATCTGTGTGGAGGGTTTCAAAGAGCACTGTGAGGAGTTCTGGCACACA
ATTAGGTACCCCAATTGGAAGCACATTTCCTGTAAGCATGCTGAAAGCGTGGAGACAGAAGGAAATGGTG
AGGACCTGCGCCTTTTCCATTCTTTTGAAGAGTTACTCCTTGAGGCTCATGGTGACTATGGATTAAGGAA
TGACTATCACATGAATCTGGGCCAATTCTTAGAATTTCTCAAGAAGCACAAAAGTGAGCATGTTTTTCAG
ATACTATTTGGTATTGAAAGCAAAAGTTCAGACTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203103 protein sequence
Red=Cloning site Green=Tags(s)

MSASVKESLQLQLLEMEMLFSMFPNQGEVKLEDVNALTNIKRYLEGTREALPPKIEFVITLQIEEPKVKI
DLQVTMPHSYPYLALQLFGRSSELDRHQQLLLNKGLTSYIGTFDPGELCVCAAIQWLQDNSASYFLNRKL
VYEPSTQAKPVKNTFLRMWIYSHHIYQQDLRKKILDVGKRLDVTGFCMTGKPGIICVEGFKEHCEEFWHT
IRYPNWKHISCKHAESVETEGNGEDLRLFHSFEELLLEAHGDYGLRNDYHMNLGQFLEFLKKHKSEHVFQ
ILFGIESKSSDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033411
ORF Size 876 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033411.2, NP_219479.1
RefSeq Size 1432 bp
RefSeq ORF 879 bp
Locus ID 112611
UniProt ID Q9UIY3, B2R7R2
Cytogenetics 6q14.1
Domains RWD
MW 33.9 kDa
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Documents
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"NM_033411" in other vectors (4)
SKU Description Size Price
RC203103L3 Lenti ORF clone of Human RWD domain containing 2A (RWDD2A), Myc-DDK-tagged 10 ug
$600.00
RC203103L4 Lenti ORF clone of Human RWD domain containing 2A (RWDD2A), mGFP tagged 10 ug
$600.00
RG203103 RWDD2A (tGFP-tagged) - Human RWD domain containing 2A (RWDD2A) 10 ug
$489.00 $500.00
SC319251 RWDD2A (untagged)-Human RWD domain containing 2A (RWDD2A) 10 ug
$300.00

Plasmid Map for RC203103

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203103
Circular map for RC203103

file-alt Citations

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