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TUSC3 (NM_178234) Human Tagged ORF Clone

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SKU
RC203082
TUSC3 (Myc-DDK-tagged)-Human tumor suppressor candidate 3 (TUSC3), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TUSC3 or NM_178234"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TUSC3
Synonyms D8S1992; M33; MagT2; MRT7; MRT22; N33; OST3A; SLC58A2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203082 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCCCGGGGCGCTCCTTCACGCCGTAGGCAAGCGGGGCGGCGGCTGCGGTACCTGCCCACCGGGA
GCTTTCCCTTCCTTCTCCTGCTGCTGCTGCTCTGCATCCAGCTCGGGGGAGGACAGAAGAAAAAGGAGAA
TCTTTTAGCTGAAAAAGTAGAGCAGCTGATGGAATGGAGTTCCAGACGCTCAATCTTCCGAATGAATGGT
GATAAATTCCGAAAATTTATAAAGGCACCACCTCGAAACTATTCCATGATTGTTATGTTCACTGCTCTTC
AGCCTCAGCGGCAGTGTTCTGTGTGCAGGCAAGCTAATGAAGAATATCAAATACTGGCGAACTCCTGGCG
CTATTCATCTGCTTTTTGTAACAAGCTCTTCTTCAGTATGGTGGACTATGATGAGGGGACAGACGTTTTT
CAGCAGCTCAACATGAACTCTGCTCCTACATTCATGCATTTTCCTCCAAAAGGCAGACCTAAGAGAGCTG
ATACTTTTGACCTCCAAAGAATTGGATTTGCAGCTGAGCAACTAGCAAAGTGGATTGCTGACAGAACGGA
TGTTCATATTCGGGTTTTCAGACCACCCAACTACTCTGGTACCATTGCTTTGGCCCTGTTAGTGTCGCTT
GTTGGAGGTTTGCTTTATTTGAGAAGGAACAACTTGGAGTTCATCTATAACAAGACTGGTTGGGCCATGG
TGTCTCTGTGTATAGTCTTTGCTATGACTTCTGGCCAGATGTGGAACCATATCCGTGGACCTCCATATGC
TCATAAGAACCCACACAATGGACAAGTGAGCTACATTCATGGGAGCAGCCAGGCTCAGTTTGTGGCAGAA
TCACACATTATTCTGGTACTGAATGCCGCTATCACCATGGGGATGGTTCTTCTAAATGAAGCAGCAACTT
CGAAAGGCGATGTTGGAAAAAGACGGATAATTTGCCTAGTGGGATTGGGCCTGGTGGTCTTCTTCTTCAG
TTTTCTACTTTCAATATTTCGTTCCAAGTACCACGGCTATCCTTATAGCTTTTTAATTAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203082 protein sequence
Red=Cloning site Green=Tags(s)

MGARGAPSRRRQAGRRLRYLPTGSFPFLLLLLLLCIQLGGGQKKKENLLAEKVEQLMEWSSRRSIFRMNG
DKFRKFIKAPPRNYSMIVMFTALQPQRQCSVCRQANEEYQILANSWRYSSAFCNKLFFSMVDYDEGTDVF
QQLNMNSAPTFMHFPPKGRPKRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRPPNYSGTIALALLVSL
VGGLLYLRRNNLEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGPPYAHKNPHNGQVSYIHGSSQAQFVAE
SHIILVLNAAITMGMVLLNEAATSKGDVGKRRIICLVGLGLVVFFFSFLLSIFRSKYHGYPYSFLIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178234
ORF Size 1041 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178234.2
RefSeq Size 3823 bp
RefSeq ORF 1044 bp
Locus ID 7991
UniProt ID Q13454
Cytogenetics 8p22
Protein Families Druggable Genome, Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
MW 39.6 kDa
Summary This gene encodes a protein that has been associated with several biological functions including cellular magnesium uptake, protein glycosylation and embryonic development. This protein localizes to the endoplasmic reticulum and acts as a component of the oligosaccharyl transferase complex which is responsible for N-linked protein glycosylation. This gene is a candidate tumor suppressor gene. Homozygous mutations in this gene are associated with autosomal recessive nonsyndromic mental retardation-7 and in the proliferation and invasiveness of several cancers including metastatic pancreatic cancer, ovarian cancer and glioblastoma multiform. provided by RefSeq, Oct 2017
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"NM_178234" in other vectors (6)
SKU Description Size Price
RC203082L1 Lenti ORF clone of Human tumor suppressor candidate 3 (TUSC3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC203082L2 Lenti ORF clone of Human tumor suppressor candidate 3 (TUSC3), transcript variant 2, mGFP tagged 10 ug
$757.00
RC203082L3 Lenti ORF clone of Human tumor suppressor candidate 3 (TUSC3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC203082L4 Lenti ORF clone of Human tumor suppressor candidate 3 (TUSC3), transcript variant 2, mGFP tagged 10 ug
$757.00
RG203082 TUSC3 (tGFP-tagged) - Human tumor suppressor candidate 3 (TUSC3), transcript variant 2 10 ug
$489.00 $657.00
SC319771 TUSC3 (untagged)-Human tumor suppressor candidate 3 (TUSC3), transcript variant 2 10 ug
$457.00

Plasmid Map for RC203082

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203082
Circular map for RC203082

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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