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Spindly (SPDL1) (NM_017785) Human Tagged ORF Clone

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SKU
RC203078
SPDL1 (Myc-DDK-tagged)-Human coiled-coil domain containing 99 (CCDC99)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $563.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Spindly or NM_017785"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Spindly
Synonyms CCDC99
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203078 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAAGAGCGACTAAAAGCTG
CACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAATTGGATAAATGTCGTAATGA
AATGATGACCATGACTGAGAGTTATGAACAAGAAAAATATACCCTTCAAAGAGAAGTTGAACTCAAGAGT
CGAATGTTAGAAAGTTTGAGCTGCGAATGTGAAGCTATTAAACAACAACAAAAAATGCACCTGGAGAAAT
TGGAAGAACAACTAAGCAGAAGCCATGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAA
AGTGGAATTAGATGAAGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTC
CTCTCTTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCTTCAGAGATGC
TGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTCAAAGAAGAAGTGAATGAACT
ACAATACAGACAAGAACAGCTAGAACTTCTTATTACTAACCTAATGCGCCAGGTAGACCGGCTTAAAGAG
GAAAAAGAGGAGCGAGAGAAAGAAGCAGTTTCTTACTATAATGCCCTAGAGAAAGCTCGTGTAGCAAATC
AAGATCTTCAGGTACAGTTGGACCAGGCACTCCAGCAAGCCTTGGATCCCAATAGTAAAGGCAACTCTTT
GTTTGCAGAGGTGGAAGATCGAAGGGCAGCAATGGAACGTCAGCTTATCAGTATGAAAGTCAAGTATCAG
TCACTAAAGAAGCAAAATGTATTTAACAGAGAACAGATGCAGAGAATGAAGTTACAAATTGCCACGTTGC
TACAGATGAAAGGGTCTCAAACTGAATTTGAGCAGCAGGAACGGTTGCTTGCCATGTTGGAGCAGAAGAA
TGGTGAAATAAAACATCTTTTAGGTGAAATTAGAAATCTGGAGAAATTTAAGAATTTATATGACAGTATG
GAATCTAAGCCTTCAGTCGACTCTGGTACTCTGGAAGATAACACCTATTATACAGATTTACTTCAGATGA
AGCTGGATAACTTAAACAAAGAAATTGAAAGCACTAAAGGTGAATTGTCCATACAGCGAATGAAAGCATT
ATTTGAGAGCCAGCGGGCTCTAGATATTGAGCGAAAACTTTTTGCAAATGAAAGATGCCTCCAGCTTTCA
GAAAGTGAAAATATGAAACTGAGAGCTAAACTAGATGAATTGAAACTAAAATATGAACCTGAAGAGACAG
TTGAAGTGCCTGTACTGAAAAAGAGGCGTGAGGTGCTCCCTGTGGATATAACCACCGCTAAAGATGCATG
TGTCAACAACAGTGCTCTCGGGGGAGAAGTTTATCGATTACCGCCTCAGAAAGAGGAGACACAGTCCTGC
CCTAACAGTTTAGAAGATAACAACTTGCAATTAGAAAAATCAGTTTCTATACACACACCAGTAGTCAGTC
TCTCTCCTCACAAAAATCTGCCCGTGGATATGCAGCTGAAGAAGGAAAAGAAATGTGTGAAACTCATAGG
AGTTCCCGCTGACGCTGAGGCCTTAAGTGAAAGAAGTGGAAACACCCCTAACTCTCCCAGGTTAGCTGCT
GAATCAAAGCTTCAAACAGAAGTTAAAGAAGGAAAAGAAACTTCAAGCAAATTGGAAAAAGAAACTTGTA
AGAAATCACACCCTATTCTATATGTGTCTTCTAAATCTACTCCAGAGACCCAGTGCCCTCAACAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203078 protein sequence
Red=Cloning site Green=Tags(s)

MEADIITNLRCRLKEAEEERLKAAQYGLQLVESQNELQNQLDKCRNEMMTMTESYEQEKYTLQREVELKS
RMLESLSCECEAIKQQQKMHLEKLEEQLSRSHGQEVNELKTKIEKLKVELDEARLSEKQLKHQVDHQKEL
LSCKSEELRVMSERVQESMSSEMLALQIELTEMESMKTTLKEEVNELQYRQEQLELLITNLMRQVDRLKE
EKEEREKEAVSYYNALEKARVANQDLQVQLDQALQQALDPNSKGNSLFAEVEDRRAAMERQLISMKVKYQ
SLKKQNVFNREQMQRMKLQIATLLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEIRNLEKFKNLYDSM
ESKPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKGELSIQRMKALFESQRALDIERKLFANERCLQLS
ESENMKLRAKLDELKLKYEPEETVEVPVLKKRREVLPVDITTAKDACVNNSALGGEVYRLPPQKEETQSC
PNSLEDNNLQLEKSVSIHTPVVSLSPHKNLPVDMQLKKEKKCVKLIGVPADAEALSERSGNTPNSPRLAA
ESKLQTEVKEGKETSSKLEKETCKKSHPILYVSSKSTPETQCPQQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017785
ORF Size 1815 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017785.3
RefSeq Size 2683 bp
RefSeq ORF 1818 bp
Locus ID 54908
UniProt ID Q96EA4
Cytogenetics 5q35.1
MW 70.1 kDa
Summary This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. provided by RefSeq, Jul 2016
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Documents
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"NM_017785" in other vectors (7)
SKU Description Size Price
RC203078L1 Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), Myc-DDK-tagged 10 ug
$863.00
RC203078L2 Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), mGFP tagged 10 ug
$863.00
RC203078L3 Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), Myc-DDK-tagged 10 ug
$863.00
RC203078L4 Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), mGFP tagged 10 ug
$863.00
RG203078 SPDL1 (tGFP-tagged) - Human coiled-coil domain containing 99 (CCDC99) 10 ug
$489.00 $763.00
SC113945 SPDL1 (untagged)-Human coiled-coil domain containing 99 (CCDC99) 10 ug
$564.00
SC321078 SPDL1 (untagged)-Human coiled-coil domain containing 99 (CCDC99) 10 ug
$564.00

Plasmid Map for RC203078

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203078
Circular map for RC203078

file-alt Citations

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