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Spindly (SPDL1) (NM_017785) Human Untagged Clone

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SKU
SC113945
SPDL1 (untagged)-Human coiled-coil domain containing 99 (CCDC99)
  $564.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Spindly
Synonyms CCDC99
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113945 sequence for NM_017785 edited (data generated by NextGen Sequencing)
ATGGAGGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAAGAGCGA
CTAAAAGCTGCACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAA
TTGGATAAATGTCGTAATGAAATGATGACCATGACTGAGAGTTATGAACAAGAAAAATAT
ACCCTTCAAAGAGAAGTTGAACTCAAGAGTCGAATGTTAGAAAGTTTGAGCTGCGAATGT
GAAGCTATTAAACAACAACAAAAAATGCACCTGGAGAAATTGGAAGAACAACTAAGCAGA
AGCCATGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAAAGTGGAATTA
GATGAAGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTC
CTCTCTTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCT
TCAGAGATGCTGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTC
AAAGAAGAAGTGAATGAACTACAATACAGACAAGAACAGCTAGAACTTCTTATTACTAAC
CTAATGCGCCAGGTAGACCGGCTTAAAGAGGAAAAAGAGGAGCGAGAGAAAGAAGCAGTT
TCTTACTATAATGCCCTAGAGAAAGCTCGTGTAGCAAATCAAGATCTTCAGGTACAGTTG
GACCAGGCACTCCAGCAAGCCTTGGATCCCAATAGTAAAGGCAACTCTTTGTTTGCAGAG
GTGGAAGATCGAAGGGCAGCAATGGAACGTCAGCTTATCAGTATGAAAGTCAAGTATCAG
TCACTAAAGAAGCAAAATGTATTTAACAGAGAACAGATGCAGAGAATGAAGTTACAAATT
GCCACGTTGCTACAGATGAAAGGGTCTCAAACTGAATTTGAGCAGCAGGAACGGTTGCTT
GCCATGTTGGAGCAGAAGAATGGTGAAATAAAACATCTTTTAGGTGAAATTAGAAATCTG
GAGAAATTTAAGAATTTATATGACAGTATGGAATCTAAGCCTTCAGTCGACTCTGGTACT
CTGGAAGATAACACCTATTATACAGATTTACTTCAGATGAAGCTGGATAACTTAAACAAA
GAAATTGAAAGCACTAAAGGTGAATTGTCCATACAGCGAATGAAAGCATTATTTGAGAGC
CAGCGGGCTCTAGATATTGAGCGAAAACTTTTTGCAAATGAAAGATGCCTCCAGCTTTCA
GAAAGTGAAAATATGAAACTGAGAGCTAAACTAGATGAATTGAAACTAAAATATGAACCT
GAAGAGACAGTTGAAGTGCCTGTACTGAAAAAGAGGCGTGAGGTGCTCCCTGTGGATATA
ACCACCGCTAAAGATGCATGTGTCAACAACAGTGCTCTCGGGGGAGAAGTTTATCGATTA
CCGCCTCAGAAAGAGGAGACACAGTCCTGCCCTAACAGTTTAGAAGATAACAACTTGCAA
TTAGAAAAATCAGTTTCTATACACACACCAGTAGTCAGTCTCTCTCCTCACAAAAATCTG
CCCGTGGATATGCAGCTGAAGAAGGAAAAGAAATGTGTGAAACTCATAGGAGTTCCCGCT
GACGCTGAGGCCTTAAGTGAAAGAAGTGGAAACACCCCTAACTCTCCCAGGTTAGCTGCT
GAATCAAAGCTTCAAACAGAAGTTAAAGAAGGAAAAGAAACTTCAAGCAAATTGGAAAAA
GAAACTTGTAAGAAATCACACCCTATTCTATATGTGTCTTCTAAATCTACTCCAGAGACC
CAGTGCCCTCAACAGTAA

Clone variation with respect to NM_017785.4
816 c=>t;1522 t=>c;1757 t=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_017785 unedited
GGTTACCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGCGAATG
GGCCGGCGACTGTGGAGTTAGCGTCCTCATGTGGACGCCCTGAGCTCCCATTAGGAGCCG
CTGGCTGCGGCAGCAGGGGACTAGCGTGAGAGTTGGCTAAAAAAAAGAAAAGAACATGGA
GGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAAGAGCGACTAAA
AGCTGCACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAATTGGA
TAAATGTCGTAATGAAATGATGACCATGACTGAGAGTTATGAACAAGAAAAATATACCCT
TCAAAGAGAAGTTGAACTCAAGAGTCGAATGTTAGAAAGTTTGAGCTGCGAATGTGAAGC
TATTAAACAACAACAAAAAATGCACCTGGAGAAATTGGAAGAACAACTAAGCAGAAGCCA
TGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAAAGTGGAATTAGATGA
AGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTCCTCTC
TTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCTTCAGA
GATGCTGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTCAAAGA
AGAAAGTGATGNAACTACATACANGACAGAACAGCTAGAACTTCTTATTACTAACCTAAT
GCGCCCAGTAGACCGGCTTNANAGAGGAAAAAGAGAGCGAGAGAAAGAGCAGTTNCTTAC
TATAATGCCCCTAGAGAAAGCTCGTGTAGCAAATCAAGATCTTTCAGTACAGTTGGNACC
AGCACTTCACCAGCCCTGGATC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_017785 unedited
TGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGAAAAAACACGGGGGTTTTTTTT
AAAATTTTAAACAAAATTTAAATCTTGTTTTTAAAACCAAAAAGGAACTTTGGTTTTTTA
AACCCTGACAAAATCCTGAAACAATTTAAGGTTTTTTTTTTAACCCACTTTTCCTTTTTT
TACAAAAAAGTTAACACCCTTCCCTTTTTTACAACATAGTTTAAAACTAAGTTTATAAAA
AAAAGTCCCTGGGAAAATTTGATGGAAGCTTTTTCAAAAACCGGGGTTTGGGGGGAAAAA
ACTTGTTTTTTTATCATTTAACCCATATCCAAGGCGGCAAAAAAAACCCTTAATTATAAC
CTTTCCCTTCAATAAAAAGTTTATTCCCGGGCAAAAAACCCCCATGAAGGGGCATCACTT
AAAAACCTCTCCAGGTTAAAGCAAACCAAATTTTGAACACTTGGTCAAAGGCCCATAAAA
ATGCCCGGCCCTGGCCCAATGAGTAAATACCCCAAAGGGGGATTTTTGTCAAATAAAAAC
AATTTTAACCACCATATAAACTTTTGGGGCAAGGGGCACCGTCCTCTTTTTAAAAAAAAG
TCTTTACTGTGGAGGGCACTGGGTCTCTGGAGTTAATTTAAAAAACCTTTTAGAATGGGG
GGGATTTCTTACAGTTTCTTTTTCCAATTGCTAGAGGNTCCTTTCCTTCTTTACTTCCGG
NGAAGCCTTGATTCACAGCTACCCTGGGAGAGTGGGGGGGGTCCCCTTTNTTTACTTAGG
GCCCAACGTAAGCGGAACCTCTAAGAGTTCACCATTTTTTCCTTCTTAGTGCTTTCCCGG
ACAATTTTGGAGGAAAG
Restriction Sites NotI-NotI |
ACCN NM_017785
Insert Size 2700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017785.2, NP_060255.2
RefSeq Size 2561 bp
RefSeq ORF 1818 bp
Locus ID 54908
UniProt ID Q96EA4
Cytogenetics 5q35.1
Summary This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. provided by RefSeq, Jul 2016
Transcript Variant: Variants 3 and 4 encode the same isoform (b).
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Documents
Resources
"NM_017785" in other vectors (7)
SKU Description Size Price
RC203078 SPDL1 (Myc-DDK-tagged)-Human coiled-coil domain containing 99 (CCDC99) 10 ug
$563.00
RC203078L1 Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), Myc-DDK-tagged 10 ug
$863.00
RC203078L2 Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), mGFP tagged 10 ug
$863.00
RC203078L3 Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), Myc-DDK-tagged 10 ug
$863.00
RC203078L4 Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), mGFP tagged 10 ug
$863.00
RG203078 SPDL1 (tGFP-tagged) - Human coiled-coil domain containing 99 (CCDC99) 10 ug
$489.00 $763.00
SC321078 SPDL1 (untagged)-Human coiled-coil domain containing 99 (CCDC99) 10 ug
$564.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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