JKAMP (NM_016475) Human Tagged ORF Clone

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SKU
RC203067
JKAMP (Myc-DDK-tagged)-Human JNK1/MAPK8-associated membrane protein (JKAMP), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol JKAMP
Synonyms C14orf100; C24orf100; CDA06; HSPC213; HSPC327; JAMP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203067 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTCGATATTCAACCAGCATGCCTTGGACTTTATTGTGGGAAGACCCTATTATTTAAAAATGGCT
CAACTGAAATATATGGAGAATGTGGGGTATGCCCAAGAGGACAGAGAACGAATGCACAGAAATATTGTCA
GCCTTGCACAGAATCTCCTGAACTTTATGATTGGCTCTATCTTGGATTTATGGCAATGCTTCCTCTGGTT
TTACATTGGTTCTTCATTGAATGGTACTCGGGGAAAAAGAGTTCCAGCGCACTTTTCCAACACATCACTG
CATTATTTGAATGCAGCATGGCAGCTATTATCACCTTACTTGTGAGTGATCCAGTTGGTGTTCTTTATAT
TCGTTCATGTCGAGTATTGATGCTTTCTGACTGGTACACGATGCTTTACAACCCAAGTCCAGATTACGTT
ACCACAGTACACTGTACTCATGAAGCCGTCTACCCACTATATACCATTGTATTTATCTATTACGCATTCT
GCTTGGTATTAATGATGCTGCTCCGACCTCTTCTGGTGAAGAAGATTGCATGTGGGTTAGGGAAATCTGA
TCGATTTAAAAGTATTTATGCTGCACTTTACTTCTTCCCAATTTTAACCGTGCTTCAGGCAGTTGGTGGA
GGCCTTTTATATTACGCCTTCCCATACATTATATTAGTGTTATCTTTGGTTACTCTGGCTGTGTACATGT
CTGCTTCTGAAATAGAGAACTGCTATGATCTTCTGGTCAGAAAGAAAAGACTTATTGTTCTCTTCAGCCA
CTGGTTACTTCATGCCTATGGAATAATCTCCATTTCCAGAGTGGATAAACTTGAGCAAGATTTGCCCCTT
TTGGCTTTGGTACCTACACCAGCCCTTTTTTACTTGTTCACTGCAAAATTTACCGAACCTTCAAGGATAC
TCTCAGAAGGAGCCAATGGACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203067 protein sequence
Red=Cloning site Green=Tags(s)

MAVDIQPACLGLYCGKTLLFKNGSTEIYGECGVCPRGQRTNAQKYCQPCTESPELYDWLYLGFMAMLPLV
LHWFFIEWYSGKKSSSALFQHITALFECSMAAIITLLVSDPVGVLYIRSCRVLMLSDWYTMLYNPSPDYV
TTVHCTHEAVYPLYTIVFIYYAFCLVLMMLLRPLLVKKIACGLGKSDRFKSIYAALYFFPILTVLQAVGG
GLLYYAFPYIILVLSLVTLAVYMSASEIENCYDLLVRKKRLIVLFSHWLLHAYGIISISRVDKLEQDLPL
LALVPTPALFYLFTAKFTEPSRILSEGANGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016475
ORF Size 933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016475.5
RefSeq Size 2429 bp
RefSeq ORF 936 bp
Locus ID 51528
UniProt ID Q9P055
Cytogenetics 14q23.1
Protein Families Transmembrane
MW 35.2 kDa
Summary May be a regulator of the duration of MAPK8 activity in response to various stress stimuli. Facilitates degradation of misfolded endoplasmic reticulum (ER) luminal proteins through the recruitment of components of the proteasome and endoplasmic reticulum-associated degradation (ERAD) system (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016475" in other vectors (5)
SKU Description Size Price
RC203067L3 Lenti ORF clone of Human JNK1/MAPK8-associated membrane protein (JKAMP), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203067L4 Lenti ORF clone of Human JNK1/MAPK8-associated membrane protein (JKAMP), transcript variant 1, mGFP tagged 10 ug
$600.00
RG203067 JKAMP (tGFP-tagged) - Human JNK1/MAPK8-associated membrane protein (JKAMP), transcript variant 1 10 ug
$489.00 $500.00
SC114240 JKAMP (untagged)-Human JNK1/MAPK8-associated membrane protein (JKAMP), transcript variant 1 10 ug
$300.00
SC320884 JKAMP (untagged)-Human JNK1/MAPK8-associated membrane protein (JKAMP), transcript variant 1 10 ug
$300.00

Plasmid Map for RC203067

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Cloning scheme for RC203067

Circular map for RC203067

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