MAGEE1 (MAGEC2) (NM_016249) Human Tagged ORF Clone

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SKU
RC203064
MAGEC2 (Myc-DDK-tagged)-Human melanoma antigen family C, 2 (MAGEC2)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAGEE1
Synonyms CT10; HCA587; MAGEE1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203064 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCCCGTTCCAGGCGTTCCATTCCGCAACGCTGACAACGACTCCCCGACCTCAGTTGAGTTAGAAG
ACTGGGTAGATGCACAGCATCCCACAGATGAGGAAGAGGAGGAAGCCTCCTCCGCCTCTTCCACTTTGTA
CTTAGTATTTTCCCCCTCTTCTTTCTCCACATCCTCTTCTCTGATTCTTGGTGGTCCTGAGGAGGAGGAG
GTGCCCTCTGGTGTGATACCAAATCTTACCGAGAGCATTCCCAGTAGTCCTCCACAGGGTCCTCCACAGG
GTCCTTCCCAGAGTCCTCTGAGCTCCTGCTGCTCCTCTTTTTCATGGAGCTCATTCAGTGAGGAGTCCAG
CAGCCAGAAAGGGGAGGATACAGGCACCTGTCAGGGCCTGCCAGACAGTGAGTCCTCTTTCACATATACA
CTAGATGAAAAGGTGGCCGAGTTAGTGGAGTTCCTGCTCCTCAAATACGAAGCAGAGGAGCCTGTAACAG
AGGCAGAGATGCTGATGATTGTCATCAAGTACAAAGATTACTTTCCTGTGATACTCAAGAGAGCCCGTGA
GTTCATGGAGCTTCTTTTTGGCCTTGCCCTGATAGAAGTGGGCCCTGACCACTTCTGTGTGTTTGCAAAC
ACAGTAGGCCTCACCGATGAGGGTAGTGATGATGAGGGCATGCCCGAGAACAGCCTCCTGATTATTATTC
TGAGTGTGATCTTCATAAAGGGCAACTGTGCCTCTGAGGAGGTCATCTGGGAAGTGCTGAATGCAGTAGG
GGTATATGCTGGGAGGGAGCACTTCGTCTATGGGGAGCCTAGGGAGCTCCTCACTAAAGTTTGGGTGCAG
GGACATTACCTGGAGTATCGGGAGGTGCCCCACAGTTCTCCTCCATATTATGAATTCCTGTGGGGTCCAA
GAGCCCATTCAGAAAGCATCAAGAAGAAAGTACTAGAGTTTTTAGCCAAGCTGAACAACACTGTTCCTAG
TTCCTTTCCATCCTGGTACAAGGATGCTTTGAAAGATGTGGAAGAGAGAGTCCAGGCCACAATTGATACC
GCAGATGATGCCACTGTCATGGCCAGTGAAAGCCTCAGTGTCATGTCCAGCAACGTCTCCTTTTCTGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203064 protein sequence
Red=Cloning site Green=Tags(s)

MPPVPGVPFRNADNDSPTSVELEDWVDAQHPTDEEEEEASSASSTLYLVFSPSSFSTSSSLILGGPEEEE
VPSGVIPNLTESIPSSPPQGPPQGPSQSPLSSCCSSFSWSSFSEESSSQKGEDTGTCQGLPDSESSFTYT
LDEKVAELVEFLLLKYEAEEPVTEAEMLMIVIKYKDYFPVILKRAREFMELLFGLALIEVGPDHFCVFAN
TVGLTDEGSDDEGMPENSLLIIILSVIFIKGNCASEEVIWEVLNAVGVYAGREHFVYGEPRELLTKVWVQ
GHYLEYREVPHSSPPYYEFLWGPRAHSESIKKKVLEFLAKLNNTVPSSFPSWYKDALKDVEERVQATIDT
ADDATVMASESLSVMSSNVSFSE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016249
ORF Size 1119 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016249.4
RefSeq Size 2008 bp
RefSeq ORF 1122 bp
Locus ID 51438
UniProt ID Q9UBF1
Cytogenetics Xq27.2
MW 41.1 kDa
Summary This gene is a member of the MAGEC gene family. It is not expressed in normal tissues, except for testis, and is expressed in tumors of various histological types. This gene and the other MAGEC genes are clustered on chromosome Xq26-q27. provided by RefSeq, Oct 2009
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Documents
Resources
"NM_016249" in other vectors (6)
SKU Description Size Price
RC203064L1 Lenti ORF clone of Human melanoma antigen family C, 2 (MAGEC2), Myc-DDK-tagged 10 ug
$986.00
RC203064L2 Lenti ORF clone of Human melanoma antigen family C, 2 (MAGEC2), mGFP tagged 10 ug
$986.00
RC203064L3 Lenti ORF clone of Human melanoma antigen family C, 2 (MAGEC2), Myc-DDK-tagged 10 ug
$986.00
RC203064L4 Lenti ORF clone of Human melanoma antigen family C, 2 (MAGEC2), mGFP tagged 10 ug
$986.00
RG203064 MAGEC2 (tGFP-tagged) - Human melanoma antigen family C, 2 (MAGEC2) 10 ug
$886.00
SC304398 MAGEC2 (untagged)-Human melanoma antigen family C, 2 (MAGEC2) 10 ug
$457.00

Plasmid Map for RC203064

Download sequence for

Cloning scheme for RC203064

Circular map for RC203064

file-alt Citations

Increased Melanoma-Associated Antigen C2 Expression Affords Resistance to Apoptotic Deathin Suspension-Cultured Tumor Cells
Park, Han, Joo et al
J Breast Cancer (2021) 24 (2), 138-152 DOI: 10.4048/jbc.2021.24.e6
Cited by 1
AI Summary: In this study, OriGene products including MAGEC2 siRNA (SR320492) and MAGEC2 antibody (TA326156) were used to deplete and detect MAGEC2 expression, respectively, enabling the authors to investigate the role of MAGEC2 in promoting resistance to apoptosis in suspension-cultured tumor cells.
FigureFigureFigure
Showing 1-1 of 1 papers.
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