PAGE4 (NM_007003) Human Tagged ORF Clone

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SKU
RC203059
PAGE4 (Myc-DDK-tagged)-Human P antigen family, member 4 (prostate associated) (PAGE4)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAGE4
Synonyms CT16.7; GAGE-9; GAGEC1; JM-27; JM27; PAGE-1; PAGE-4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203059 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCACGAGTGAGATCAAGATCCAGAGGAAGAGGAGATGGTCAGGAGGCTCCCGATGTGGTTGCAT
TCGTGGCTCCCGGTGAATCTCAGCAAGAGGAACCACCAACTGACAATCAGGATATTGAACCTGGACAAGA
GAGAGAAGGAACACCTCCGATCGAAGAACGTAAAGTAGAAGGTGATTGCCAGGAAATGGATCTGGAAAAG
ACTCGGAGTGAGCGTGGAGATGGCTCTGATGTAAAAGAGAAGACTCCACCTAATCCTAAGCATGCTAAGA
CTAAAGAAGCAGGAGATGGGCAGCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203059 protein sequence
Red=Cloning site Green=Tags(s)

MSARVRSRSRGRGDGQEAPDVVAFVAPGESQQEEPPTDNQDIEPGQEREGTPPIEERKVEGDCQEMDLEK
TRSERGDGSDVKEKTPPNPKHAKTKEAGDGQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007003
ORF Size 306 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007003.4
RefSeq Size 563 bp
RefSeq ORF 309 bp
Locus ID 9506
UniProt ID O60829
Cytogenetics Xp11.23
MW 11.2 kDa
Summary This gene is a member of the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in prostate and prostate cancer. It is also expressed in other male and female reproductive tissues including testis, fallopian tube, uterus, and placenta, as well as in testicular cancer and uterine cancer. The protein encoded by this gene shares sequence similarity with other GAGE/PAGE proteins, and also belongs to a family of CT (cancer-testis) antigens. The protein may play a role in benign and malignant prostate diseases. A related pseudogene is located on chromosome 7. Alternate splicing results in multiple transcript variants. provided by RefSeq, Jan 2016
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Documents
Resources
"NM_007003" in other vectors (6)
SKU Description Size Price
RC203059L1 Lenti ORF clone of Human P antigen family, member 4 (prostate associated) (PAGE4), Myc-DDK-tagged 10 ug
$450.00
RC203059L2 Lenti ORF clone of Human P antigen family, member 4 (prostate associated) (PAGE4), mGFP tagged 10 ug
$450.00
RC203059L3 Lenti ORF clone of Human P antigen family, member 4 (prostate associated) (PAGE4), Myc-DDK-tagged 10 ug
$450.00
RC203059L4 Lenti ORF clone of Human P antigen family, member 4 (prostate associated) (PAGE4), mGFP tagged 10 ug
$450.00
RG203059 PAGE4 (tGFP-tagged) - Human P antigen family, member 4 (prostate associated) (PAGE4) 10 ug
$489.00
SC128094 PAGE4 (untagged)-Human P antigen family, member 4 (prostate associated) (PAGE4) 10 ug
$150.00

Plasmid Map for RC203059

Download sequence for

Cloning scheme for RC203059

Circular map for RC203059

file-alt Citations

The Cancer/Testis Antigen PAGE4, a Regulator of c-Jun Transactivation, is Phosphorylated by Homeodomain-interacting Protein Kinase 1, a Component of the Stress-response Pathway
Mooney, Qiu, Kim et al
Biochemistry (2014)
Cited by 47
AI Summary: In this study, the OriGene product "PAGE4-Myc-FLAG" was used as a source of tagged PAGE4 protein to investigate its phosphorylation and role in regulating c-Jun transactivation in prostate cancer research.
Snippet: according to the manufacturer's instructions. 6Myc-PAGE4 was constructed by cloning PAGE4 WT and T51A into the pCS3-6Myc vector and PAGE4-Myc-FLAG was purchased (Origene, Rockville, MD). Recombinant Proteins ... Cite SaveSaving
Showing 1-1 of 1 papers.
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