DERL2 (NM_016041) Human Tagged ORF Clone

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SKU
RC203046
DERL2 (Myc-DDK-tagged)-Human Der1-like domain family, member 2 (DERL2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DERL2
Synonyms CGI-101; derlin-2; DERtrin-2; F-LAN-1; F-LANa; FLANa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203046 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTACCAGAGCTTGCGGCTGGAGTACCTGCAGATCCCACCGGTCAGCCGCGCCTACACCACTGCCT
GCGTCCTCACCACCGCCGCCGTGCAGTTGGAATTGATCACACCTTTTCAGTTGTACTTCAATCCTGAATT
AATCTTTAAACACTTTCAAATATGGAGATTAATCACCAACTTCTTATTTTTTGGGCCAGTTGGATTCAAT
TTTTTATTTAACATGATTTTTCTATATCGTTACTGTCGAATGCTAGAAGAAGGCTCTTTCCGAGGTCGGA
CAGCAGACTTTGTATTTATGTTCCTTTTTGGTGGATTCTTAATGACCCTTTTTGGTCTGTTTGTGAGCTT
AGTTTTCTTGGGCCAGGCCTTTACAATAATGCTCGTCTATGTGTGGAGCCGAAGGAACCCCTATGTCCGC
ATGAACTTCTTCGGCCTTCTCAACTTCCAGGCCCCCTTTCTGCCCTGGGTGCTCATGGGATTTTCCTTGT
TGTTGGGGAACTCAATCATTGTGGACCTTTTGGGTATTGCAGTTGGACACATATATTTTTTCTTGGAAGA
TGTATTTCCCAATCAACCTGGTGGAATAAGAATTCTGAAAACACCATCTATTTTGAAAGCTATTTTTGAT
ACACCAGATGAGGATCCAAATTACAATCCACTACCTGAGGAACGGCCAGGAGGCTTCGCCTGGGGTGAGG
GCCAGCGGCTTGGAGGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203046 protein sequence
Red=Cloning site Green=Tags(s)

MAYQSLRLEYLQIPPVSRAYTTACVLTTAAVQLELITPFQLYFNPELIFKHFQIWRLITNFLFFGPVGFN
FLFNMIFLYRYCRMLEEGSFRGRTADFVFMFLFGGFLMTLFGLFVSLVFLGQAFTIMLVYVWSRRNPYVR
MNFFGLLNFQAPFLPWVLMGFSLLLGNSIIVDLLGIAVGHIYFFLEDVFPNQPGGIRILKTPSILKAIFD
TPDEDPNYNPLPEERPGGFAWGEGQRLGG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016041
ORF Size 717 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016041.5
RefSeq Size 1156 bp
RefSeq ORF 720 bp
Locus ID 51009
UniProt ID Q9GZP9
Cytogenetics 17p13.2
Domains DER1
Protein Families Transmembrane
MW 27.6 kDa
Summary Proteins that are unfolded or misfolded in the endoplasmic reticulum (ER) must be refolded or degraded to maintain the homeostasis of the ER. DERL2 is involved in the degradation of misfolded glycoproteins in the ER (Oda et al., 2006 PubMed 16449189).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_016041" in other vectors (6)
SKU Description Size Price
RC203046L1 Lenti ORF clone of Human Der1-like domain family, member 2 (DERL2), Myc-DDK-tagged 10 ug
$600.00
RC203046L2 Lenti ORF clone of Human Der1-like domain family, member 2 (DERL2), mGFP tagged 10 ug
$600.00
RC203046L3 Lenti ORF clone of Human Der1-like domain family, member 2 (DERL2), Myc-DDK-tagged 10 ug
$600.00
RC203046L4 Lenti ORF clone of Human Der1-like domain family, member 2 (DERL2), mGFP tagged 10 ug
$600.00
RG203046 DERL2 (tGFP-tagged) - Human Der1-like domain family, member 2 (DERL2) 10 ug
$489.00 $500.00
SC114512 DERL2 (untagged)-Human Der1-like domain family, member 2 (DERL2) 10 ug
$300.00

Plasmid Map for RC203046

Download sequence for

Cloning scheme for RC203046

Circular map for RC203046

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