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PIGP (NM_153682) Human Tagged ORF Clone

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SKU
RC203040
PIGP (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PIGP or NM_153682"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PIGP
Synonyms DCRC; DCRC-S; DEE55; DSCR5; DSRC; EIEE55; PIG-P
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203040 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGAAAATTCACCGTCGCCATTGCCAGAAAGAGCGATTTATGGCTTTGTTCTTTTCTTAAGCTCCC
AATTTGGCTTCATACTTTACCTCGTGTGGGCCTTTATTCCTGAATCTTGGCTAAACTCTTTAGGTTTAAC
CTATTGGCCTCAAAAATATTGGGCAGTTGCATTACCTGTCTACCTCCTTATTGCTATAGTAATTGGCTAC
GTGCTCTTGTTTGGGATTAACATGATGAGTACCTCTCCACTCGACTCCATCCATACAATCACAGATAACT
ATGCAAAAAATCAACAGCAGAAGAAATACCAAGAGGAGGCCATTCCAGCCTTAAGAGATATTTCTATTAG
TGAAGTAAACCAAATGTTCTTTCTTGCAGCCAAAGAACTTTACACCAAAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203040 protein sequence
Red=Cloning site Green=Tags(s)

MVENSPSPLPERAIYGFVLFLSSQFGFILYLVWAFIPESWLNSLGLTYWPQKYWAVALPVYLLIAIVIGY
VLLFGINMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFFLAAKELYTKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153682
ORF Size 402 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153682.3
RefSeq Size 804 bp
RefSeq ORF 405 bp
Locus ID 51227
UniProt ID P57054
Cytogenetics 21q22.13
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
MW 15.4 kDa
Summary This gene encodes an enzyme involved in the first step of glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. The encoded protein is a component of the GPI-N-acetylglucosaminyltransferase complex that catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). This gene is located in the Down Syndrome critical region on chromosome 21 and is a candidate for the pathogenesis of Down syndrome. This gene has multiple pseudogenes and is a member of the phosphatidylinositol glycan anchor biosynthesis gene family. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Feb 2016
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"NM_153682" in other vectors (5)
SKU Description Size Price
RC203040L3 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC203040L4 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2, mGFP tagged 10 ug
$450.00
RG203040 PIGP (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2 10 ug
$489.00
SC110438 PIGP (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2 10 ug
$150.00
SC320893 PIGP (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class P (PIGP), transcript variant 2 10 ug
$150.00

Plasmid Map for RC203040

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203040
Circular map for RC203040

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